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Research for the effect of vaso-activators in neonatal vascular smooth muscle

血管激活剂对新生儿血管平滑肌作用的研究

基本信息

批准号：
15591888
负责人：
IEIRI Satoshi
金额：
$ 1.79万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591888/
关键词：
Persistent pulmonary hypertension of newborn Vascular smooth muscle Vasodilator Pulmonary artery Intracellular Ca concentration Mechanical ventilation Cytokine Stress 細胞内カルシウム濃度

项目摘要

The aim of this study is to elucidate the effect of stress (mechanical ventilation) in neonatal vascular smooth muscle using a rat model. Pregnant Sprague-Dawley rats were treated by experimental protocols on day 21 of gestation. We used the following two groups : The fetal stabilization group (FS-group) which consisted of neonatal rats treated by fetal stabilization. They received morphine hydrochloride (3mg/kg) via the placenta by means of maternal intra-abdominal injection before undergoing a caesarean section. In the control group (C-group), they received no morphine hydrochloride during the perinatal period. Tracheal intubations were performed in neonates after caesarean section in both groups. All neonatal rats were managed under mechanical ventilation for 4 hours. We collected the samples at birth and at 4 hours after birth in both groups and then measured the serum Tumor-necrosis-factor-alpha (TNF-α), Interleukin-6 (IL-6) concentrations and the TNF-α concentration in BALF by ELISA. The specimens obtained from the right lung were immunohistochemically stained with anti-mouse TNF-α polyclonal antibody. The levels of serum TNF-α at birth and IL-6 at 4 hours after birth in the C-group increased, in comparison to that in the FS-group. At birth in the C-group, the TNF-α concentration in BALF tended to be higher than that in the FS-group. TNF-α positive cells were identified in the bronchial muscular layers, arterial media and externa in both groups. The staining intensity in the FS-group was weaker than that in the C-group both at birth and at 4 hours after birth. Our result suggests that the regulation of the production of inflammatory cytokines may regulate the action of vascular smooth muscle.

本研究的目的是阐明应激（机械通气）的影响，在新生血管平滑肌使用大鼠模型。妊娠Sprague-Dawley大鼠在妊娠第21天通过实验方案进行处理。我们使用以下两组：胎儿稳定组（FS组），由经胎儿稳定处理的新生大鼠组成。在进行剖腹产前，通过母体腹腔内注射的方式经胎盘接受盐酸吗啡（3 mg/kg）。对照组（C组）围产期不给予盐酸吗啡。两组新生儿剖宫产后均行气管插管。所有新生大鼠在机械通气下管理4小时。分别于出生时及生后4 h采血，采用ELISA法检测两组患儿血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）浓度及BALF中TNF-α浓度。取右肺标本，用抗小鼠TNF-α多克隆抗体进行免疫组化染色。C组出生时血清TNF-α和出生后4 h血清IL-6水平均高于FS组。出生时C组BALF中TNF-α浓度有高于FS组的趋势。TNF-α阳性细胞分布于两组的支气管肌层、动脉中层和外膜。FS组在出生时和出生后4小时的染色强度均弱于C组。我们的结果表明，调节炎症细胞因子的产生可能调节血管平滑肌的活动。