Disturbance of the immune responses by periodontopathic bacterial virulence factors and host defense activity of salivary proteins

牙周病细菌毒力因子和唾液蛋白宿主防御活性对免疫反应的干扰

• 批准号: 15591948
• 负责人: KATO Tetsuo
• 金额: $ 2.11万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591948/
• 关键词: periodontopathic bacteria; endotoxin; cytokine; mouse; neonatal; saliva; biofilm; cystatin; 免疫; IgE; 唾液タンパク質

Cytokines produced in response to periodontopathic bacteria clearly play a key role in the periodontal diseases. Recently, it has been suggested that periodontal disease and periodontal pathogens in pregnant women may be a significant risk factor for preterm low birth weight. many animal experiments showed a potential association between periodontopathic bacteria and reduced fetal weight. In this study, the effect of neonatally exposure to periodontopathic bacterial LPSs on cytokine production and humoral immune response in mice was investigated. Newborn BALB/c mice were given subcutaneously injected of 20 ng lipopolysaccharides (LPSs) of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans or Escherichia coli daily for 2 days, starting within 24 h after birth. The treated mice were given intraperitoneally injections of BSA at 180 and 187 days of age. Seventeen hours after the each injection, the mice were bled and sera were separated. Their sera were tested for anti-BSA reactivity in an ELISA system. The levels of IL-4,IL-5,IL-6,IFNγ and IgE in the sera were measured using the ELISA kits. The mean IL-4,IL-5 and IL-6 levels in the sera of neonatally P.gingivalis LPS treated mice were significantly higher than those of the controls. The mean serum IgE level of mice neonatally treated with P.gingivalis LPS were higher than that of control. However, in all cases, no significant difference was noted between neonatally LPS of A.actinomycetemcomitans or E. coli treated mice and control mice. These data suggest that neonatally exposure to P.gingivalis LPS induces changes of immunological responses when the mice reach maturity.Salivary cystatins act for host defense mechanism. We investigated the effects of periodontopathic bacteria and their endotoxin on cystatin production by human cultured cells. The present study exhibited that periodontopathic bacteria did not have influence on the cystatin C production.

牙周致病菌引起的细胞因子在牙周病中起着重要作用。最近，有研究表明，孕妇牙周疾病和牙周病原体可能是早产低出生体重的一个重要危险因素。许多动物实验表明牙周病细菌和胎儿体重减轻之间存在潜在的联系。本研究旨在探讨牙周病细菌脂多糖对小鼠细胞因子产生和体液免疫应答的影响。新生BALB/c小鼠从出生后24 h内开始每天皮下注射20 ng牙龈卟啉单胞菌、伴放线放线杆菌或大肠杆菌的脂多糖（LPS），连续2 d。接受治疗的小鼠在180天和187日龄时腹腔内注射BSA。每次注射后17小时，将小鼠流血的并分离血清。在ELISA系统中测试其血清的抗BSA反应性。用ELISA试剂盒检测血清中IL-4、IL-5、IL-6、IFNγ和IgE水平。牙龈卟啉单胞菌LPS处理组小鼠血清中IL-4、IL-5和IL-6水平均显著高于对照组。经牙龈卟啉单胞菌LPS处理的小鼠血清IgE水平明显高于对照组。然而，在所有的情况下，没有显着差异，注意到放线放线菌或E. coli处理的小鼠和对照小鼠。提示牙龈卟啉单胞菌脂多糖的体外暴露可引起成年小鼠免疫应答的改变，唾液中的半胱氨酸蛋白酶抑制剂参与了机体的防御机制。我们研究了牙周病细菌及其内毒素对人培养细胞产生半胱氨酸蛋白酶抑制剂的影响。本研究表明，牙周致病菌对Cystatin C的产生没有影响。

项目成果

Murine monoclonal antibody which can distinguish cystatins SA1 and SA2.

鼠单克隆抗体，可区分半胱氨酸蛋白酶抑制剂SA1和SA2。

• 发表时间: 2005
• 期刊: Mol. Immunol. 42・10
• 作者: Ito;T.
• 通讯作者: T.

Cystatin SA, a cysteine proteinase inhibitor, induces interferon-γ expression in CD4-positive T cells

Cystatin SA，一种半胱氨酸蛋白酶抑制剂，诱导 CD4 阳性 T 细胞中干扰素-γ 表达

• 发表时间: 2004
• 期刊: Biol.Chem. 385
• 作者: Kato;T.;Ito;T.;Imatani;T.;Minaguchi;K.;Saitoh;E.;Okuda;K.
• 通讯作者: K.

Murine monoclonal antibody which can distinguish cystatins SA1 and SA2

可区分半胱氨酸蛋白酶抑制剂 SA1 和 SA2 的鼠单克隆抗体

• 发表时间: 2005
• 期刊: Mol.Immunol. 42(10)
• 作者: Ito;T.;Komiya-Ito;A.;Okuda;K.;Minaguchi;K.;Saitoh;E.;Yamada;S.;Kato;T.
• 通讯作者: T.

Periodontopathic bacterial endotoxin-induced tumor necrosis factora production was inhibited by exercise in mice.

小鼠运动可抑制牙周病细菌内毒素诱导的肿瘤坏死因子的产生。

• 发表时间: 2006
• 期刊: FEMS Immunol.Med.microbial. (in press)
• 作者: 豊野孝;瀬田祐司;片岡真司;豊島邦昭;Shinji Hiyama;K.Hirota;Y.Hosokawa;D.Viducic;Keiji Murakami;Kato T
• 通讯作者: Kato T

Periodontopathic bacterial endotoxin-induced tumor necrosis factor α production was inhibited by execise in mice

小鼠运动可抑制牙周病细菌内毒素诱导的肿瘤坏死因子α的产生

• 发表时间: 2006
• 期刊: FEMS Immunol.Med.Microbiol. in press
• 作者: Kato;T.;Kaneko;S.;Kimizuka;R.;Okuda;K.
• 通讯作者: K.