Immunological Study of Lipopolysaccharide from Periodontopathic Bacteria.

牙周病细菌脂多糖的免疫学研究。

• 批准号: 01570997
• 负责人: KATO Tetsuo
• 金额: $ 1.34万
• 依托单位: Tokyo dental college
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570997/
• 关键词: Periodontopathic Bacteria; Lipopolysaccharide; Lipid A; Mitogenic Activity; Polyclonal B-cell Activation; Monoclonal Antibody; Antiーidiotype Antibody; 歯周病; Fusobacterium nucleatum; マイトジェン活性

The antigenic structure of lipopolysaccharides (LPS) of Fusobacterium nucleatum and Eikenella corrodens was analyzed with prepared murine monoclonal antibodies. Three monoclonal antibodies (isotype IgM) against F. nucleatum LPS were crossーreactive with LPS from E. corrodens. Two out of 3 monoclonal antibodies (isotype IgM) produced by immunization with E. corrodens LPS crossーreacted with LPS from F. nucleatum. Western immunoblotting analysis demonstrated that the 5 antibodies reacted with the common core epitope of LPSs from F. mucleatum and E. corrodens. Another monoclonal antibody (isotype IgG3) against E. corrodens LPS reacted with the O-side chain epitope of E. corrodens LPS and crossーreacted with the core epitopes of LPSs from Fusobacterium necrophorum and Capnocytophaga ochracea. Four monoclonal antibodies (isotype IgM) against E. corrodens LPS reacted with lipid A preparations from E. corrodens, Escherichia coli and Salmonella minesota.It was found that the mitogenic activity and polyclonal B-cell activation of LPSs from E. corrodens and F. nucleatum were comparable to those of E. coli LPS. And these activities were partially inhibited by the monoclonal antibodies against LPS.We produced antiーidiotype antibodies to antibody to lipid A from E. corrodens. The sera of mice and rabbits immunized with the antiーidiotype antibodies revealed that the antiーidiotype antibody induced the production of IgG antibodies which crossー reacted with lipid A. Immunization of mice with the antiーidiotype antibody resulted in prevention of lethal toxicity from LPS.

用制备的鼠源单克隆抗体分析了具核梭杆菌和腐蚀艾肯氏菌脂多糖的抗原结构。3株抗F.核质LPS与E.腐蚀用E. corrotens LPS与F.核质Western免疫印迹分析表明，这5株抗体均与F. macucleatum和E.腐蚀另一株抗E.腐蚀菌LPS与E.结果表明，该菌株与坏死梭杆菌和赭色二氧化碳噬纤维菌的LPS核心表位发生交叉反应。4株抗E.腐蚀菌LPS与E.结果表明，E. corrodens、Escherichia coli和Salmonella minesota的LPS具有促有丝分裂活性和多克隆B细胞活化活性。corrodens和F. nucleatum与E. coli LPS。用抗脂多糖单克隆抗体（McAb）部分抑制E.腐蚀用该抗体免疫小鼠和家兔血清，结果表明该抗体可诱导产生IgG抗体，并与脂质A发生交叉反应。用抗LPS独特型抗体免疫小鼠可预防LPS的致死毒性。

项目成果

K.OKUDA: "Neuraminidaseーenhanced attchment of Bacteroides intermedius to human erythrocytes and buccal epitherial cells." Infect.Immun.57. 1635-1637 (1989)

K.OKUDA：“中间类杆菌与人红细胞和口腔上皮细胞的神经氨酸酶增强附着。Infect.Immun.57（1989）”

T. Kato: "Antibacterial effects of listerine on oral bacteria." Bull. Tokyo Dent. Coll.31. 301-307 (1990)

T. Kato：“李施德林对口腔细菌的抗菌作用。”

G. Yamane: "The disinfection of NJ-D on oral mucosal membrane and tooth extraction wound" Shikwa Gakuho. 90. 1167-1177 (1990)

G. Yamane：“NJ-D 对口腔粘膜和拔牙伤口的消毒”Shikwa Gakuho。

T.KATO,I.TAKAZOE and K.OKUDA: "Protection of mice against the lethal toxicity of a lipopolysaccharide(LPS) by immunization with antiーidiotype antibody to a monoclonal antibody to lipid A from Eikenella corrodens LPS." Infection and Immunity. 58. 416-420 (

T.KATO、I.TAKAZOE 和 K.OKUDA：“通过针对腐蚀艾肯菌 LPS 感染和免疫的脂质 A 的抗独特型抗体进行免疫，保护小鼠免受脂多糖 (LPS) 的致命毒性。” 58.416-420（

石原 和幸 内藤 祐子 加藤 哲男 高添 一郎 奥田 克爾 江口 徹 中島 光一 松田 尚樹 山崎 恭子 長谷川 健二 水道 裕久 杉原 邦夫: "歯周病原菌の酵素活性を指標とした迅速診断法(SKー013) ーSKー013の特性と酵素特異性の検討ー" 日本歯周病学会会誌. 32. 224-232 (1990)

Kazuyuki Ishihara、Yuko Naito、Tetsuo Kato、Ichiro Takazoe、Katsuji Okuda、Toru Eguchi、Koichi Nakajima、Naoki Matsuda、Kyoko Yamazaki、Kenji Hasekawa、Hirohisa Suido、Kunio Sugihara：“以牙周病原菌的酶活性为指标的快速诊断方法(SK-013) - SK-013 的特性和酶特异性检查”日本牙周病学会杂志 32. 224-232 (1990)。