Studies on the effect of low intensity ultrasound to accelerate bone formation around implants

低强度超声加速种植体周围骨形成效果的研究

• 批准号: 15592058
• 负责人: HAMANO Tohru
• 金额: $ 2.18万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592058/
• 关键词: implant; bone formation; ultrasound; osseointegration

We investigated if low-intensity pulsed ultrasound (LIPUS) accelerates bone formation around implants. Rats were given titanium screw (Micro PLUS, Leibinger, Germany) implanted in their bilateral tibiae. LIPUS was daily administrated to right tibia, and not to left tibia as an internal control.Experiment 1 : Morphological study with a micro focus computerized tomography (micro CT)Six rats were divided into 2 groups: Three rats received LIPUS treatment for 4 days, and the others for 7 days. Then tibiae were harvested and were scanned using a micro CT (ELE SCAN, Nittetsu elex ). For morphological observation of bone formation around implants, 3D-images were constructed using image analyzing soft. Due to the halation of implants, the micro CT images were not obvious. In results, no apparent difference was observed between bilateral tibiae in either 4-days group or 7-days group.Experiment 2 : Mechanical evaluation for fixation of implant in tibiaeTo evaluate the mechanical stability of implant, removal torque of screw in 6 rats were measured using a torque gauge (ATG6CN, TOHNICHI) after unilateral LIPUS application for 7 days. The removal torque of screw was smaller than the insert torque in each rat, and displayed no apparent differences between bilateral tibiae. It was considered the cortical bone of tibiae was destroyed so much that screw could not be fixed in bone.In this study, the effect of LIPUS to accelerate bone formation around implants was not shown. In further study, the other animal model for implant study was needed.

我们研究了低强度脉冲超声（LIPUS）是否加速种植体周围的骨形成。将钛螺钉（Micro PLUS，Leibinger，德国）植入大鼠双侧胫骨。实验1：用微焦点计算机断层扫描（microCT）进行的形态学研究将6只大鼠分为2组：3只大鼠接受LIPUS治疗4天，其余大鼠接受7天。然后收获组织，并使用微CT（ELE SCAN，Nittetsu elex）扫描。应用图像分析软件对种植体周围骨形成进行三维图像分析。由于种植体的光晕，显微CT图像不明显。实验2：胫骨内种植体固定的力学评价为了评价种植体的力学稳定性，在单侧LIPUS应用7天后，使用扭矩仪（ATG 6CN，TOHNICHI）测量6只大鼠的螺钉移除扭矩。每只大鼠的螺钉旋出扭矩均小于螺钉旋入扭矩，双侧胫骨间无明显差异。认为胫骨皮质骨破坏严重，螺钉无法固定在骨内，本研究未显示LIPUS加速种植体周围骨形成的作用。在进一步的研究中，需要另一种用于植入研究的动物模型。