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Investigation of dynamics of intracellular calcium ion induced by lidocaine on porcine arterial smooth muscle

利多卡因诱导猪动脉平滑肌细胞内钙离子动态的研究

基本信息

批准号：
15592128
负责人：
SATOH Ken-ichi
金额：
$ 1.86万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

项目摘要

1. To deduce the contractile. mechanism of Noradrenaline and adrenaline on porcine lingual arterial smooth muscle, we investigated changes in isometric tension and intracellular calcium level ([Ca^<2+>]_i) simultaneously. We assessed the relationship between the isometric tension and [Ca^<2+>]_i induced by 10^<-7>, 10^<-6>, 10^<-5>, 10^<-4>, 10^<-3>, 0.1, 0.5, 1.0, 2.0, 4.0, 6.0, 10.0, 20.0uM NA and Ad.As results,(1) NA and Ad increased the contraction and [Ca^<2+>]_i in a concentration-dependent manner.(2) NA and Ad induced transient increase in [Ca^<2+>]_i followed by sustained levels, while they induced rapid and sustained contractions.(3) In the contraction by NA Ad, phasic contraction depends on the release of Ca^<2+> from the intracellular Ca^<2+> store and tonic contraction depends on the influx of extracellular Ca^<2+>2. To deduce the effects of Lidocaine on lingual, pulmonary and coronary arterial smooth muscle, we measured simultaneously the isometric contraction and [Ca^<2+> … More ]_i induced by 0.5, 1.0, 10.0, 20.0, 50μg/ml lidocaine after 40mM KCl, 5μM NA and Ad administeredAs results,(1) 1.0μg/ml lidocaine induced increases in tension and [Ca^<2+>]_i when 40mM KCl, 5μM NA and Ad were administered to lingual and pulmonary arterial smooth muscle.(2) In coronary artery, the administration of 5μM NA and Ad induced no response and when 40mM KCl was administered, 1.0μg/ml lidocaine induced increases in tension and [Ca^<2+>]_i.3. To deduce the effects of mepivacaine on lingual, pulmonary and coronary arterial smooth muscle, we measured simultaneously the isometric contraction and [Ca^<2+>]_i induced by 0.4μM, 4.0μM, 40μM, 0.4mM, 4.0mM mepivacaine after 40mM KCl administered.As results,(1) In lingual and coronary arterial smooth muscle, 4μM mepivacaine induced increase in tension and [Ca^<2+>]_i and 40μM, 0.4mM, 4.0mM mepivacaine induced decrease in tension and [Ca^<2+>]_i.(2) In pulmonary arterial smooth muscle, 0.4 and 4μM mepivacaine induced increase in tension and [Ca^<2+>]_i and 40μM, 0.4mM, 4.0mM mepivacaine induced decrease in these. Less

1.以推断收缩力。为探讨去甲肾上腺素和肾上腺素对猪舌动脉平滑肌的作用机制，同时观察了舌动脉平滑肌等长收缩张力和细胞内钙离子浓度（[Ca^<2+>] i）的变化。结果表明<-7><-6><-5><-4><-3>：（1）NA和Ad浓度依赖性地增加离体心肌细胞的收缩力和[Ca ^2 +] i; (2)NA和Ad引起[Ca^<2+>] i短暂升高，随后持续升高，而它们引起快速和持续收缩。(3)在NA Ad引起的收缩中，时相性收缩依赖于细胞内Ca^2+库的Ca^2+释放，而紧张性收缩依赖于细胞外Ca^2+的内流。为探讨利多卡因对舌、肺和冠状动脉平滑肌的作用，我们同时测定了舌、肺和冠状动脉平滑肌的等长收缩和[Ca^<2+> ...更多信息结果表明：（1）1.0 μ g/ml利多卡因使舌动脉和肺动脉平滑肌在40 mMKCl、5 μ M NA和Ad作用下，张力和[Ca^（2+）]_i增加; (2)在冠状动脉中，5μM NA和Ad不引起反应，而在40 mM KCl中，1.0μg/ml利多卡因引起张力和[Ca^（2+）]_i增加。为探讨甲哌卡因对舌、肺和冠状动脉平滑肌的作用，我们同时测定了0.4μM、4.0μM、40μ M、0.4mM、4.0mM甲哌卡因在40 mM KCl作用下引起的舌、肺和冠状动脉平滑肌等长收缩和[Ca^<2+]_i的变化。4μM甲哌卡因引起张力和[Ca^2+] i增加，40μM、0.4mM、4.0mM甲哌卡因引起张力和[Ca^2+] i降低。(2)在肺动脉平滑肌，0.4和4μM甲哌卡因引起张力增加，[Ca^（2+）]_i和40μM、0.4mM、4.0mM甲哌卡因引起[Ca^（2+）]_i降低。少