Investigation of dynamics of intracellular calcium ion induced by lidocaine on porcine arterial smooth muscle

1.推论收缩。去甲肾上腺素和肾上腺素对猪舌动脉平滑肌的作用机制，我们同时观察了等长张力和细胞内钙离子浓度的变化。我们评估了10^-7；-6&gt；，10^&lt；-4；-4；，10^&lt；-3&gt；，0.1，0.5，1.0，2.0，4.0，6.0，10.0，20.0uM NA和Ad.结果：(1)NA和Ad增加了收缩和[Ca^&lt；2+&gt；]。(2)NA和Ad引起短暂的[Ca^&lt；2+&gt；]_i升高，并引起持续的收缩。(3)在NA Ad的收缩中，相性收缩依赖于细胞内钙离子的释放，而紧张性收缩依赖于细胞外钙的内流。2+&>…40 mM KCl0.5、1.0、10.0、20.0、50μg/ml利多卡因引起的张力增加，40 mM KCl1.0μg/ml利多卡因引起的张力升高，40 mM KCl5μg/ml5μMNA和Ad引起的[Ca^&lt；2+&gt；]_i增加。(2)冠状动脉注射5μ-MNA和Ad无反应，40 mM KCl1.0μg/ml利多卡因引起的张力和[Ca^&lt；]_I.3.为探讨甲比卡因对舌、肺和冠状动脉平滑肌的作用，我们同时测定了40μKCl0.4μM、4.0μM、40μM、0.4 mM、4.0 mM甲比卡因引起的等长收缩和[Ca^&lt；2+&gt；]_i。结果：(1)在舌和冠状动脉平滑肌，4μM甲比卡因引起张力升高，[Ca^&lt；2+&gt；]_i和40 mM、0.4 mM、4.0 mM甲哌卡因引起张力降低和[Ca^&lt；2+&gt；]_i降低。(2)在肺动脉平滑肌上，0.4和4μM甲哌卡因引起张力升高，[Ca~(2+)]_i和40μM、0.4 mM、4.0 mM甲哌卡因引起张力降低。较少

1. To deduce the contractile. mechanism of Noradrenaline and adrenaline on porcine lingual arterial smooth muscle, we investigated changes in isometric tension and intracellular calcium level ([Ca^<2+>]_i) simultaneously. We assessed the relationship between the isometric tension and [Ca^<2+>]_i induced by 10^<-7>, 10^<-6>, 10^<-5>, 10^<-4>, 10^<-3>, 0.1, 0.5, 1.0, 2.0, 4.0, 6.0, 10.0, 20.0uM NA and Ad.As results,(1) NA and Ad increased the contraction and [Ca^<2+>]_i in a concentration-dependent manner.(2) NA and Ad induced transient increase in [Ca^<2+>]_i followed by sustained levels, while they induced rapid and sustained contractions.(3) In the contraction by NA Ad, phasic contraction depends on the release of Ca^<2+> from the intracellular Ca^<2+> store and tonic contraction depends on the influx of extracellular Ca^<2+>2. To deduce the effects of Lidocaine on lingual, pulmonary and coronary arterial smooth muscle, we measured simultaneously the isometric contraction and [Ca^<2+> … More ]_i induced by 0.5, 1.0, 10.0, 20.0, 50μg/ml lidocaine after 40mM KCl, 5μM NA and Ad administeredAs results,(1) 1.0μg/ml lidocaine induced increases in tension and [Ca^<2+>]_i when 40mM KCl, 5μM NA and Ad were administered to lingual and pulmonary arterial smooth muscle.(2) In coronary artery, the administration of 5μM NA and Ad induced no response and when 40mM KCl was administered, 1.0μg/ml lidocaine induced increases in tension and [Ca^<2+>]_i.3. To deduce the effects of mepivacaine on lingual, pulmonary and coronary arterial smooth muscle, we measured simultaneously the isometric contraction and [Ca^<2+>]_i induced by 0.4μM, 4.0μM, 40μM, 0.4mM, 4.0mM mepivacaine after 40mM KCl administered.As results,(1) In lingual and coronary arterial smooth muscle, 4μM mepivacaine induced increase in tension and [Ca^<2+>]_i and 40μM, 0.4mM, 4.0mM mepivacaine induced decrease in tension and [Ca^<2+>]_i.(2) In pulmonary arterial smooth muscle, 0.4 and 4μM mepivacaine induced increase in tension and [Ca^<2+>]_i and 40μM, 0.4mM, 4.0mM mepivacaine induced decrease in these. Less