The cross talking of the gene and regeneration in regeneration of periodontal tissue - main role of CBFA-1 -

牙周组织再生中基因与再生的交叉对话-CBFA-1的主要作用-

• 批准号: 15592141
• 负责人: TANAKA Hiroshi
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592141/
• 关键词: CBFA-1; CDF; BMP; root formation; odontoblast; Hertwig上皮鞘; GDF; Runz2

Deficiency of Core binding factor A-1(CBFA-1) causes cleidocranial disostosis and play important role differentiation of the osteoblasts precursor cells to the osteoblast. CBFA-1 is expressed in many tissues and controls differentiation and growth of cells. Expression of CBFA-1 mRNA is regulated by the expression of BMP.In the present study, cross talking of BMP and CBFA-1 mRNA were studied by in situ hybridization and immunohistochemistry.Under sodium pentobarbital anesthesia, Wistar rats (40 g) were perfused via the left ventricles with 4% paraformaldehyde solution diluted in 0.025 M phosphatebuffer (pH 7.4). The embryos were demineralized with 0.1M EDTA (pH 7.4), and after dehydration in ethanol tissue, they were embedded in paraffin and then cut longitudinally into 5 μm thick slices along the long axis of incisors.These animal experiments were approved by the Institutional Ethical Committee for Animal Experiments and properly carried out under the control of the Guidelines for Animal Experimentation in Nihon University School of Dentistry.During the development of oralmaxillo-facial region, expression of BMP was appeared in preosteoblasts of the region of the bone and tooth formation. Expression of CBFA-1 mRNA was detected slightly later stage than those of BMP mRNA. CBFA-1 mRNA was expressed in the osteoblasts and osteocytes. GDF was strongly expressed in the cementoblasts.During root formation, BMP mRNA was expressed in the cells of epithelial root sheath of Hertwig (ERSH). On the other hand, CBFA-1 mRNA was expressed in the dental palilla cells and preodontoblasts facing the ERSH.Expression of BMP stimulates transcription level of CBFA-1 mRNA. Therefore, the expression of BMP from the ERSH induce the expression of CBFA-1 of n the dental palilla cells and preodontoblasts facing the ERSH. The cross talking of BMP and CBF-1 regulate the differentiation of the cells forming root formation and bone.

核心结合因子A-1（CBFA-1）缺乏导致锁骨颅裂，并在成骨细胞前体细胞向成骨细胞分化中起重要作用。CBFA-1在许多组织中表达，并控制细胞的分化和生长。CBFA-1 mRNA的表达受BMP的表达调控。本研究采用原位杂交和免疫组化的方法研究了BMP与CBFA-1 mRNA的相互作用。在戊巴比妥钠麻醉下，用0.025 M磷酸盐缓冲液（pH 7.4）稀释4%多聚甲醛溶液经左心室灌注Wistar大鼠（40 g）。胚胎用0.1M EDTA （pH 7.4）脱矿，经乙醇组织脱水后包埋石蜡，沿门牙长轴纵向切成5 μm厚的切片。这些动物实验得到了动物实验伦理委员会的批准，并在日本大学牙科学院动物实验指南的控制下进行。在口腔颌面区发育过程中，骨和牙形成区成骨前细胞中出现BMP的表达。CBFA-1 mRNA的表达比BMP mRNA的表达稍晚。CBFA-1 mRNA在成骨细胞和骨细胞中表达。GDF在成水泥细胞中强烈表达。在根形成过程中，Hertwig （ERSH）上皮根鞘细胞表达BMP mRNA。另一方面，CBFA-1 mRNA在面向ERSH的牙柄细胞和前成牙细胞中表达。BMP的表达刺激CBFA-1 mRNA的转录水平。因此，ERSH表达BMP可诱导面对ERSH的牙柄细胞和前成牙细胞表达CBFA-1。BMP和CBF-1的相互作用调节了骨和根形成细胞的分化。

项目成果

マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について

CBFA-1和BMP-4在小鼠颌面部发育中的定位

• 发表时间: 2003
• 期刊: 日大歯学 77
• 作者: 稲毛稔彦;田中博;他15名
• 通讯作者: 他15名

Localization of CBFA-1 and BMP-4 during Oral Maxillofaciai Development in Mice.

CBFA-1 和 BMP-4 在小鼠口腔颌面发育过程中的定位。

• 发表时间: 2003
• 期刊: Nihon University Dental journal Vol.77-2
• 作者: Toshihiko Inage;Hiroshi Tanaka;Shinichiro Oida;et al.
• 通讯作者: et al.

エナメル芽細胞におけるCBFA-1/Runx2の局在

CBFA-1/Runx2 在成釉细胞中的定位

• 发表时间: 2003
• 期刊: 日本大学歯学部研究紀要 31
• 作者: 桑田文幸;稲毛稔彦;他3名
• 通讯作者: 他3名

Localization Of CBFA-1/Runx2 in Ameloblasls.

CBFA-1/Runx2 在成釉细胞中的定位。

• 期刊: Nihon University School of Dentistry Research Bulletin Vol.31
• 作者: Fumiyuki Kuwata;Toshihiko Inage;et al.
• 通讯作者: et al.