Mechanisms of ectopic orofacial pain following peripheral nerve injury.

周围神经损伤后异位口面部疼痛的机制。

• 批准号: 15592149
• 负责人: TOKUNAGA Atsushi
• 金额: $ 2.18万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592149/
• 关键词: ectopic pain; trigeminal ganglion; extracellular signal-related kinase; nerve injury; immunohistochemistry; glia; rat; immunohistochemistry

It has been reported that the injury of the 3rd branch of trigeminal nerve induced abnormal sensations in the area of the 2nd branch of trigeminal nerve. We hypothesized that the induction of the abnormal sensations were involved in the intra-ganglionic signaling via glial cells and satellite cells in the trigeminal ganglion. After transection of the inferior alveolar nerve, ED-1 positive cells were detected in through out of the trigeminal ganglion, not only around the ATF3 positive neurons. The induction of ED-1 positive cells was speculated to be mediated by cytokines such as TNFα, however, the induction of NF-κB was not detected. Furthermore, in order to investigate whether the activation of neurons in the 1st or 2nd branch of trigeminal ganglion could be induced, the phosphorylation of extracellular signal-related kinase(ERK) of the neurons was discussed. However, after the transection of inferior alveolar nerve, mechanical stimulation to the whisker pad by von Frey filaments did not induce the phosphorylated ERK in the trigeminal ganglion neurons.

据报道，三叉神经第3支损伤可引起三叉神经第2支区域的感觉异常。我们假设异常感觉的诱导涉及通过三叉神经节中的神经胶质细胞和卫星细胞进行的神经节内信号传导。横断下牙槽神经后，在三叉神经节外检测到 ED-1 阳性细胞，而不仅仅是 ATF3 阳性神经元周围。推测 ED-1 阳性细胞的诱导是由 TNFα 等细胞因子介导的，但未检测到 NF-κB 的诱导。此外，为了研究能否诱导三叉神经节第一或第二分支神经元的激活，对神经元细胞外信号相关激酶(ERK)的磷酸化进行了探讨。然而，横断下牙槽神经后，冯弗雷丝对胡须垫的机械刺激并没有诱导三叉神经节神经元中磷酸化的ERK。

项目成果

Shimode.N.: "The effects of dexmedetomidine and halothane on Fos expression in the spinal dorsal horn using a rat postoperative pain model."Neurosci.Lett.. 343. 45-48 (2003)

Shimode.N.：“使用大鼠术后疼痛模型，右美托咪定和氟烷对脊髓背角 Fos 表达的影响。”Neurosci.Lett.. 343. 45-48 (2003)

Nishizaki.K.: "Induction of neuronal nitric oxide synthase by sympathetic denervation is mediated via α2-adrenoceptors in the jejunal myenteric plexus"Brain Res.. 965. 121-129 (2003)

Nishizaki.K.：“通过空肠肌间丛中的 α2-肾上腺素受体介导去交感神经支配神经元一氧化氮合酶”Brain Res.. 965. 121-129 (2003)

Contribution of sensitized P2X receptors in inflamed tissue to the mechanical hypersensitivity revealed by phosphorylated ERK in DRG neurons

• DOI: 10.1016/j.pain.2003.12.034
• 发表时间: 2004-04-01
• 期刊: PAIN
• 影响因子: 7.4
• 作者: Dai, Y;Fukuoka, T;Noguchi, K
• 通讯作者: Noguchi, K

Role of mitogen-activated protein kinase activation in injured and intact primary afferent neurons for mechanical and heat hypersensitivity after spinal nerve ligation

• DOI: 10.1523/jneurosci.3388-04.2004
• 发表时间: 2004-11-10
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Obata, K;Yamanaka, H;Noguchi, K
• 通讯作者: Noguchi, K

Differential activation of MAPK in injured and uninjured DRG neurons following chronic constriction injury of the sciatic nerve in rats

• DOI: 10.1111/j.1460-9568.2004.03754.x
• 发表时间: 2004-12-01
• 期刊: EUROPEAN JOURNAL OF NEUROSCIENCE
• 影响因子: 3.4
• 作者: Obata, K;Yamanaka, H;Noguchi, K
• 通讯作者: Noguchi, K