Involvement of immune system to the inferior alveolar nerve injury.

免疫系统参与下牙槽神经损伤。

• 批准号: 13672134
• 负责人: TOKUNAGA Atsushi
• 金额: $ 2.3万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672134/
• 关键词: neuritis; peripheral nerve; cytokine; glia; dorsal root ganglion; nerve injury; rat; immunohistochemistry

Eliav et al. (Pain 83 162-182, 1999) reported that peripheral neuritis of the rat sciatic nerve induced hyperalgesia and allodynia.We modified the Eliav's model in order to prevent physical stimulation to the sciatic nerve. Oxycel was directly placed on the sciatic nerve without any touch of instruments, and saturated with Complete Freund's Adjuvant (CFA). ED-1 (a marker for macrophages), TNFalpha and IL-1beta immunoreactivity were detected from post-operative day 1 by immunohistochemistry. TNFalpha and IL-1beta immunoreactive cells were thought to be Schwann cells. Myelin basic protein was abolished on the sciatic nerve. This suggests that demuelination was occurred in the sciatic nerve. Furthermore, dorsal root ganglion neurons expressed Neuropeptide Y, GAP-43 and ATF3, which were well known to be expressed on axotomized DRG neurons.Those findings suggested that activating of peripheral immune system might induce peripheral nerve injury.

Eliav等（Pain 83 162-182，1999）报道了大鼠坐骨神经的外周神经炎引起痛觉过敏和异常性疼痛。将Oxycel直接放置在坐骨神经上，不接触任何器械，并用完全弗氏佐剂（CFA）饱和。从术后第1天开始，通过免疫组织化学检测艾德-1（巨噬细胞的标志物）、TNF α和IL-1 β免疫反应性。TNF α和IL-1 β免疫反应细胞被认为是雪旺细胞。坐骨神经上的髓鞘碱性蛋白被消除。这表明坐骨神经发生了脱髓鞘。此外，背根神经节神经元表达神经肽Y、GAP-43和ATF 3，这些都是已知的在轴突切断的DRG神经元上表达的，这些结果表明，外周免疫系统的激活可能导致周围神经损伤。

项目成果

Dai. Y.: "Phosphorylation of extracellular signal-regulated kinase in primary afferent neurons by noxious stimuli and its involvement in peripheral sensitization"J. Neurosci.. 22. 7734-7745 (2002)

戴.

Liu Y., Tachibana T., Dai Y., Kondo E., Fukuoka T., Yamanaka H., and Noguchi K.: "Heme Oxygenase-1 Expression Following Spinal Cord Injury; the Induction in Activated Neutrophils"J. Neurotrauma. 19. 479-490 (2002)

Liu Y.、Tachibana T.、Dai Y.、Kondo E.、Fukuoka T.、Yamanaka H. 和 Noguchi K.：“脊髓损伤后血红素加氧酶-1 表达；活化中性粒细胞的诱导”J。

Liu.Y.: "Heme Oxygenase-1 Expression Following Spinal Cord Injury ; the Induction in Activated Neutrophils"J.Neurotrauma.. 19. 479-490 (2002)

Liu.Y.：“脊髓损伤后血红素加氧酶-1 的表达；活化中性粒细胞的诱导”J.Neurotrauma.. 19. 479-490 (2002)

Kondo. E.: "Involvement of glutamate receptors on hyperexcitability of wide dynamic range neurons in the gracile nucleus of the rats with experimental mononeuropathy"Pain. 95. 153-163 (2002)

近藤。

Dai.Y.: "Phosphorylation of extracellular signal-regulated kinase in primary afferent neurons by noxious stimuli and its involvement in peripheral sensitization"J.Neurosci.. 22. 7734-7745 (2002)

Dai.Y.：“伤害性刺激对初级传入神经元细胞外信号调节激酶的磷酸化及其参与外周敏化”J.Neurosci.. 22. 7734-7745 (2002)