权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Experimental infective endocarditis induced by human supragingival dental plaque

人龈上牙菌斑诱发实验性感染性心内膜炎

基本信息

批准号：
15592211
负责人：
NAGATA Emi
金额：
$ 2.18万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Human dental plaque, which consists of numerous species of microorganisms, is thought to work as a pathogen not only in the oral cavity but also in other body sites. However, the pathogenicity of human dental plaque itself in systemic disease is not fully examined. The ability of human supragingival dental plaque to induce infective endocarditis was examined using the rat model. The bacteria were isolated from the locus of infection and the ability of the isolate to induce endocarditis was evaluated. In total, 15 out of 27 catheterized rats survived after intravenous injections with human supragingival dental plaque suspensions containing 3×10^6 CFU of bacterial cells In surviving rats, infiected vegetations were formed in all except one rat. The microbial composition of the infected vegetations was very simple compared to the respective dental plaque inoculates. In all infected vegetations examined, Streptococcus oralis comprised the majority of the isolates. In rats affected with infective endocarditis, the aortic sinus was filled with fibrinous vegetation containing bacteria. Numerous inflammatory cells infiltrated the aortic valve and the aorta adjacent to the valve. A cell suspension of S.oralis isolated from the infected vegetation was capable of inducing endocarditis lesions in all catheterized rats when inoculated at 5×10^6 CFU per rat. The results suggest that human supragingival dental plaque is an important source of dissemination of pathogen, which causes infective endocarditis.

人类牙菌斑由多种微生物组成，被认为不仅在口腔中而且在身体其他部位也是病原体。然而，人类牙菌斑本身在全身性疾病中的致病性尚未得到充分检验。使用大鼠模型检查了人龈上牙菌斑诱发感染性心内膜炎的能力。从感染部位分离细菌，并评估分离物诱发心内膜炎的能力。静脉注射含有3×10^6 CFU细菌细胞的人龈上牙菌斑悬浮液后，27只插管大鼠中总共有15只存活。在存活的大鼠中，除一只大鼠外，所有大鼠均形成感染赘生物。与相应的牙菌斑接种物相比，受感染植被的微生物组成非常简单。在检查的所有受感染植被中，口腔链球菌占分离株的大多数。在患有感染性心内膜炎的大鼠中，主动脉窦充满了含有细菌的纤维素赘生物。大量炎症细胞浸润主动脉瓣和瓣膜附近的主动脉。当以每只大鼠 5×10^6 CFU 接种时，从受感染植被中分离出的口链球菌细胞悬液能够在所有插入导管的大鼠中诱导心内膜炎病变。研究结果提示，人类龈上牙菌斑是病原体传播的重要来源，可引起感染性心内膜炎。