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Study of verification and origin of a novel medullary respiratory neuron group (para-facial respiratory group)

一种新型髓质呼吸神经元群（面旁呼吸群）的验证及起源研究

基本信息

批准号：
16500208
负责人：
ONIMARU Hiroshi
金额：
$ 2.18万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500208/
关键词：
respiratory center medulla rat rhythm generation facial nerve central chemoreceptor optical recording in vitro オピオイド中枢化学受容

项目摘要

Aim of this study was to reveal cellular level entity (e.g. distribution, morphology, physiological and pharmacological characteristics) and the function of novel medullary respiratory neuron group (para-facial respiratory group, pFRG ; Onimaru ＆ Homma, 2003). For this analysis, we used the brainstem-spinal cord preparation isolated form newborn rats. The present study revealed the following : 1)The pFRG consisted predominantly of pre-inspiratory (Pre-I) neurons. 2)Cell soma of the pFRG/Pre-I neuron was located close to the ventral surface, immediately below the pia membrane, at the level of the facial nucleus. 3)Activity of the pFRG/Pre-I neurons was not inhibited by μ-opiate agonist, DAMGO. 4)Activity of the pFRG/Pre-I neurons was reflected in the facial nerve activity. 5)When the medulla was transversely cut between the pFRG and the pre-Botzinger complex (PBC), each block of the medulla could produced spontaneous rhythmic activity under the appropriate experimental conditions. 6)These rhythmic activity corresponded to the pFRG/Pre-I (in the rostral block) and PBC/inspiratory (in the caudal block) neuron activity. 7)Rhythm of the pFRG/Pre-I neurons was depressed by increasing extracellular potassium ion concentration, application of a persistent sodium channel blocker (riluzole) or lowering CO2 pressure, whereas the PBC/inspiratory activity was more resistant to these manipulations. 8)When the medulla was cut through the midline, rhythmic activity of the pFRG/Pre-I neurons was maintained independently in the right and left medulla, but PBC-inspiratory activity was stopped. These findings showed that mechanism of rhythm generation by the pFRG/Pre-I neurons are essentially different from that of PBC-inspiratory neurons and suggested that the pFRG/Pre-I neurons determine basic respiratory rhythm under normal experimental condition with normal (or lower) external potassium concentration.

本研究的目的是揭示新的延髓呼吸神经元群(面旁呼吸群，pFRG；Onimaru&Homma，2003)的细胞水平实体(如分布、形态、生理和药理学特征)和功能。在这项分析中，我们使用了从新生大鼠身上分离出来的脑干-脊髓标本。本研究发现：1)pFRG主要由吸气前(Pre-I)神经元组成。2)pFRG/Pre-I神经元胞体靠近面神经核腹侧面，紧靠软脑膜下方。3)μ阿片激动剂DAMGO不抑制pFRG/Pre-I神经元的活动。4)面神经活动反映了pFRG/Pre-I神经元的活动。5)当切断延髓位于pFRG和Pre-Botzinger Complex(PBC)之间时，在适当的实验条件下，延髓的每一块都能产生自发的节律性活动。6)这些节律性活动与pFRG/Pre-I(吻侧阻滞)和PBC/吸气(尾侧阻滞)神经元活动相对应。7)增加细胞外钾离子浓度、应用持续性钠通道阻滞剂(利鲁唑)或降低二氧化碳分压可抑制pFRG/Pre-I神经元的节律，而PBC/吸气活动对这些操作更具抵抗力。8)切断延髓中线后，左右两侧延髓内pFRG/Pre-I神经元的节律性活动独立维持，但PBC吸气活动停止。这些结果表明，pFRG/Pre-I神经元的节律产生机制与PBC-吸气神经元的节律产生机制有本质的不同，提示pFRG/Pre-I神经元在正常实验条件下，在正常(或低)外钾浓度的情况下，决定基本的呼吸节律。

项目成果

期刊论文数量（76）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Facilitation of respiratory rhythm by aμ-opioid agonist in newborn rat pons-medulla-spinal cord preparations.

aμ-阿片受体激动剂在新生大鼠脑桥-髓质-脊髓制剂中促进呼吸节律。

DOI：
发表时间：
2005
期刊：
Neurosci Lett 375
影响因子：
0
作者：
Tanabe A;Fujii T;Onimaru H
通讯作者：
Onimaru H

Electrophysiological properties of rostral ventrolateral medulla neurons in angiotensin II 1a receptor knockout mice

DOI：
10.1161/01.hyp.0000173421.97463.ac
发表时间：
2005-08-01
期刊：
HYPERTENSION
影响因子：
8.3
作者：
Matsuura, T;Kumagai, H;Saruta, T
通讯作者：
Saruta, T

In vitro visualization of respiratary neuron activity in the newborn mouse ventral medulla

新生小鼠腹延髓呼吸神经元活动的体外可视化

DOI：
发表时间：
2004
期刊：
Dev.Brain Res. 153
影响因子：
0
作者：
Onimaru H.;Arata A.;他
通讯作者：
他

Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column

DOI：
10.1152/ajpregu.00254.2006
发表时间：
2007-02-01
期刊：
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
影响因子：
2.8
作者：
Iigaya, Kamon;Kumagai, Hiroo;Itoh, Hiroshi
通讯作者：
Itoh, Hiroshi

Homma I Developmental changes in the spatio-temporal pattern of respiratory neuron activity in the medulla of late fetal rat.

Homma I 晚期胎鼠髓质呼吸神经元活动时空模式的发育变化。