Ionic mechanisms of CO2 sensitivity in Phox2b neurons as the medullary central chemoreceptor

作为髓质中枢化学感受器的 Phox2b 神经元 CO2 敏感性的离子机制

• 批准号: 22500296
• 负责人: ONIMARU Hiroshi
• 金额: $ 2.16万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500296/
• 关键词: 呼吸中枢; 延髄; 中枢化学受容器; Phox2b; 呼吸リズム形成

It has been suggested that Phox2b expressing cells including pFRG/Pre-I neurons in the rostral ventrolateral medulla function as the central chemoreceptors (i.e. CO2 sensor). In the present study, we investigated neuronal mechanisms of high CO2 reception of pFRG/Pre-I neurons, using the brainstem-spinal cord preparation from newborn rats. The CO2 sensitivity of pFRG/Pre-I neurons was preserved even after blockade of Na+channels and Ca2+channels, and the membrane depolarization induced by hypercapnic stimulation was mainly due to the closing of K+channels. We also found that Phox2b-expressing cells including pFRG/Pre-I neurons in the parafacial region of the rostral ventral medulla tended to assemble around capillary blood vessels. We confirmed that TASK1,2 and 3 channels were less significantly involved in hypercapnic responses of pFRG/Pre-I neurons. Our findings demonstrate that the pFRG/Pre-I neurons possess postsynaptic mechanisms that are directly responsible to high CO2 stimulation.

已经提出，在延髓头端腹外侧区中包括pFRG/Pre-I神经元的Phox 2b表达细胞作为中枢化学感受器（即CO2传感器）起作用。在本研究中，我们研究了高CO2接收的pFRG/前I神经元的神经机制，使用脑干脊髓制备从新生大鼠。pFRG/Pre-I神经元的CO2敏感性在Na+通道和Ca ~（2+）通道阻断后仍保持不变，高碳酸刺激引起的膜去极化主要是由于K ~+通道关闭所致。我们还发现，Phox 2b表达细胞，包括pFRG/前-I神经元在parfacial区域的头端腹侧延髓往往聚集在毛细血管周围。我们证实，TASK 1，2和3通道参与pFRG/Pre-I神经元的高碳酸反应不太明显。我们的研究结果表明，pFRG/Pre-I神经元具有突触后机制，直接负责高CO2刺激。

项目成果

Phox2b, RTN/pFRG neurons and respiratory rhythmogenesis

• DOI: 10.1016/j.resp.2009.03.007
• 发表时间: 2009-08-31
• 期刊: RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
• 影响因子: 2.3
• 作者: Onimaru, Hiroshi;Ikeda, Keiko;Kawakami, Kiyoshi
• 通讯作者: Kawakami, Kiyoshi

Long-lasting shortening of inspiratory burst duration by treatment with eugenol

通过丁子香酚治疗可持久缩短吸气爆发持续时间

• 发表时间: 2013
• 作者: Sayumi Kotani;Hiroshi Onimaru
• 通讯作者: Hiroshi Onimaru

The inhibitory peptide galanin decreases episodic bursting, and promotes quantal slowing, of C4 activity in the neonatal rat

抑制肽甘丙肽可减少新生大鼠 C4 活性的间歇性爆发并促进量子减慢

• 发表时间: 2012
• 作者: Ta ra G. Bautista;Darko Spirovski;Angelina Y. Fong;Paul M. Pilowsky;Hiroshi Onimaru
• 通讯作者: Hiroshi Onimaru

Neurophysiological and histological properties of parafacial respiratory neurons

面旁呼吸神经元的神经生理学和组织学特性

• 发表时间: 2011
• 作者: Onimaru;H. Tanabe;A
• 通讯作者: A

Optical imaging of propofol‐induced central respiratory depression in medulla–spinal cord preparations from newborn rats

• DOI: 10.1111/j.1440-1681.2011.05480.x
• 发表时间: 2011-03
• 期刊: Clinical and Experimental Pharmacology and Physiology
• 影响因子: 2.9
• 作者: M. Kashiwagi;Y. Osaka;H. Onimaru;J. Takeda