Molecular mechanisms underlying progenitor migration following methylmercury exposure in the developing brain

发育中大脑暴露于甲基汞后祖细胞迁移的分子机制

• 批准号: 16500214
• 负责人: KAKITA Akiyoshi
• 金额: $ 2.37万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500214/
• 关键词: Progenitor migration; Brain development; methylmercury; Fetal Minamata disease; Molecular pathology; DNA chip

To understand the effects of methylmercury (MeHg) on neuronal and glial progenitor migration in the developing cerebral cortex, we examined profile alterations in gene expression in the telencephalic mantle following fetal or neonatal administration of MeHg, wher an apparent disrupition of the progenitor migration had been elucidated histopathologically. We applied extracted mRNA samples to two kinds of DNA microarrays, which had been made under different biotechnological concepts. Then, we performed analysis of the plenty of data according to the bioinformatics methodologies. We selected 11 molecules, which could be regarded as candidates of specific molecular cues closely associated with the migration disturbance underlying MeHg neurotoxicity.

为了了解甲基汞（甲基汞）对发育中的大脑皮层神经元和神经胶质祖细胞迁移的影响，我们研究了胎儿或新生儿甲基汞给药后端脑地幔中基因表达的变化，其中一个明显的破坏祖细胞迁移的组织病理学已被阐明。我们将提取的mRNA样品应用于两种不同生物技术概念下制备的DNA微阵列。然后，我们根据生物信息学的方法对大量的数据进行了分析。我们选择了11个分子，这可以被视为候选人的特定分子线索密切相关的迁移障碍的甲基汞神经毒性。

项目成果

Influences of dopaminergic lesion on epidermal growth factor-ErbB signals in Parkinson's disease and its model: neurotrophic implication in nigrostriatal neurons

• DOI: 10.1111/j.1471-4159.2005.03073.x
• 发表时间: 2005-05-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Iwakura, Y;Piao, YS;Nawa, H
• 通讯作者: Nawa, H

Prefrontal Abnormality of Schizophrenia Revealed by DNA Microarray: Impact on Glial and Neurotrophic Gene Expression

• DOI: 10.1196/annals.1316.011
• 发表时间: 2004-10
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: T. Sugai;M. Kawamura;S. Iritani;K. Araki;T. Makifuchi;China Imai;R. Nakamura;A. Kakita;H. Takahashi;H. Nawa

ErbB1 receptor ligands attenuate the expression of synaptic scaffolding proteins, GRIP1 and SAP97, in developing neocortex.

ErbB1 受体配体减弱发育中的新皮质中突触支架蛋白 GRIP1 和 SAP97 的表达。

• 发表时间: 2005
• 期刊: Neuroscience 136(4)
• 作者: Yokomaku D;他6名
• 通讯作者: 他6名

Pathological heterogeneity of the precentral gyrus in Pick's disease: a study of 16 autopsy cases.

皮克病中央前回的病理异质性：16例尸检病例的研究。

• 发表时间: 2006
• 期刊: Acta Neuropathol. (In press)
• 作者: TANIGUCHI M;MASUDA T;FUKUYAMA M;KATAOKA H;MISHINA M;YAGINUMA H;WAANABE M;SHIMIZU T;K.Tsuchiya
• 通讯作者: K.Tsuchiya

Sporadic fatal insomnia with spongiform degeneration in the thalamus and widespread PrP^<Sc> deposition in the brain.

散发性致命性失眠，伴有丘脑海绵状变性和大脑中广泛的 PrP^<Sc> 沉积。

• 发表时间: 2005
• 期刊: Neuropathology 25・2
• 作者: Tanaka M;Iijima N;Miyamoto Y;Fukusumi S;- Itoh Y;Ozawa h;Ibata Y;Y.-S.Piao
• 通讯作者: Y.-S.Piao