Development of the Taste sensor using molecularly imprinted polymers and application for bitterness-suppression

以甲基环酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，制备了辛可尼定（CD）的分子印迹聚合物。CD印迹MAA-co-EDMA聚合物可以分别对相关化合物奎尼丁和奎宁以及目标分子进行印迹。我们决定用这种CD印迹聚合物体系作为筛选奎宁抑制剂的平台，结合常数的大小表明了受试化合物对CD印迹聚合物的抑制作用。L-Arg的缔合常数计算为12.6 mol/<-1>L。在L-Lys的情况下，不能确定缔合常数。这表明L-Arg对CD的MIP具有比L-Lys更强的亲和力。因此，在MIP研究中获得的缔合常数可以提供测试物质的苦味抑制作用的指示。人的味觉感觉测试的结果与MIP数据相关良好。味觉传感器与MIPs联用的方法具有特异性和更高的灵敏度。

Molecularly imprinted polymers (MIPs) for cinchonidine (CD) were prepared using methacyclic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a crosslinker. CD-imprinted MAA-co-EDMA polymers can recognaize the related compounds quinidine and quinine, respectively, as well as the target molecules. We decided to use this CD-imprinted polymer system as a platform for screening suppressing agents for quinine.The magnitudes of the association constants indicate the remarking of the inhibitory effect of the test chemicals on the CD-imprinted polymers. The association constants were calculated to be 12.6 mol^<-1>L for L-Arg. In the case of L-Lys, no association constant could be determined. This indicates that L-Arg have stronger affinities for the MIP for CD than L-Lys. The association constants obtained in an MIP study may therefore provide an indication of the bitterness-suppressing effects of test substances. The results of human gustatory sensation tests correlated well with the MIP data. The method using Taste sensor with MIPs seems to have specificity and more excellent sensitivity.