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Identification of Japanese isolates of spotted fever group rickettsiae

斑疹热群立克次体日本分离株的鉴定

基本信息

批准号：
63044102
负责人：
UCHIDA Takahiro
金额：
$ 2.56万
依托单位：
University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044102/
关键词：
Sptted Fever Group Rickettsia Rickettsia japonica Spotted Fever リケッチア感染症

项目摘要

Spotted fever group (SFG) rickettsiae isolated from patients in the southeastern part of Shikoku, Japan, were analyzed to identify the species by use of mouse antisera. Antigenic differences between five strains of Japanese isolates and standard strains of pathogenic SFG rickettsiae were examined by calculating the specificity difference (SPD). the SPD between each pair of Japanese strains showed their antigenic identity, while the SPD between Japanese strains and pathogenic standard strains of SFG indicated that Japanese strains are clearly distinct from R.akari, R.australis, R.conorii, R.rickettsii, and R.sibirica. It was also demonstrated that Japanese strains are different from Thai tick typhus rickettsia (strain TT-118) isolated from a tick, known only in the Orient. Monoclonal antibody analysis resulted in no cross-reaction between Japanese strain and standard strains but positive reactions of Japanese strains with monoclonal antibody to Japanese strain. Comparison of the Japanese strain and standard strains of SFG rickettsiae by Western immunoblotting with mouse antiserum to Japanese strain demonstrated that the Japanese strain are distinct from any of the standard strain of SFG rickettsiae. The results obtained in this study demonstrated that the Japanese isolates of SFG rickettsiae belong to a single species distinct from any SFG rickettsiae known to be pathogenic to humans. We proposed the taxonomic name Rickettsia japonica sp. nov. pertaining to Japan, since that is the country in which it was first isolated.

利用小鼠抗血清对日本四国市东南部患者分离到的斑点热组立克次体进行鉴定。通过计算特异性差异（SPD）检验5株日本分离株与标准株的抗原性差异。日本株与SFG致病标准株的SPD差异表明日本株与r.a akari、r.s australis、r.c conorii、r.t rickettsii和r.s sibirica有明显的差异。结果还表明，日本蜱虫斑疹伤寒与泰国蜱虫立克次体（TT-118株）不同，该菌株仅在东方发现。单克隆抗体分析结果显示，日本菌株与标准株无交叉反应，而日本菌株与日本菌株的单克隆抗体呈阳性反应。用小鼠抗血清免疫印迹法对日本株和标准株进行比较，结果表明日本株与标准株具有明显的差异。本研究的结果表明，日本分离的SFG立克次体属于一个单一的物种，不同于已知的任何对人类致病的SFG立克次体。我们建议将立克次体命名为日本立克次体，因为它最早是在日本被分离出来的。