Identification of Japanese isolates of spotted fever group rickettsiae

斑疹热群立克次体日本分离株的鉴定

基本信息

批准号：
63044102
负责人：
UCHIDA Takahiro
金额：
$ 2.56万
依托单位：
University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044102/
关键词：
Sptted Fever Group Rickettsia Rickettsia japonica Spotted Fever リケッチア感染症

项目摘要

Spotted fever group (SFG) rickettsiae isolated from patients in the southeastern part of Shikoku, Japan, were analyzed to identify the species by use of mouse antisera. Antigenic differences between five strains of Japanese isolates and standard strains of pathogenic SFG rickettsiae were examined by calculating the specificity difference (SPD). the SPD between each pair of Japanese strains showed their antigenic identity, while the SPD between Japanese strains and pathogenic standard strains of SFG indicated that Japanese strains are clearly distinct from R.akari, R.australis, R.conorii, R.rickettsii, and R.sibirica. It was also demonstrated that Japanese strains are different from Thai tick typhus rickettsia (strain TT-118) isolated from a tick, known only in the Orient. Monoclonal antibody analysis resulted in no cross-reaction between Japanese strain and standard strains but positive reactions of Japanese strains with monoclonal antibody to Japanese strain. Comparison of the Japanese strain and standard strains of SFG rickettsiae by Western immunoblotting with mouse antiserum to Japanese strain demonstrated that the Japanese strain are distinct from any of the standard strain of SFG rickettsiae. The results obtained in this study demonstrated that the Japanese isolates of SFG rickettsiae belong to a single species distinct from any SFG rickettsiae known to be pathogenic to humans. We proposed the taxonomic name Rickettsia japonica sp. nov. pertaining to Japan, since that is the country in which it was first isolated.

分析了日本西库库（Shikoku）东南部患者分离的斑点发烧组（SFG）立克，分析了通过使用小鼠抗血清来识别该物种。通过计算特异性差异（SPD），检查了五种日本分离株和病原SFG立克菌的标准菌株之间的抗原差异。每对日本菌株之间的SPD均显示出它们的抗原性身份，而SFG的日本菌株和致病性标准菌株之间的SPD表明，日本菌株与R.Akari，R.Australis，R.Conorii，R.RickettSII和R.Sibirica明显不同。还证明，日本菌株与泰式typhus鼠尾车（菌株TT-118）不同，从tick中分离出来，仅在东方闻名。单克隆抗体分析导致日本菌株和标准菌株之间没有交叉反应，而是日本菌株与日本菌株的阳性反应。西方免疫印迹用小鼠抗血清与日本菌株的日本菌株和标准菌株的比较表明，日本菌株与SFG人力素的任何标准菌株不同。在这项研究中获得的结果表明，SFG立克西亚的日本分离株属于与任何SFG Rickettsiae不同的单一物种，已知对人类具有致病性。我们提出了分类名称Rickettsia Japonica sp。十一月。与日本有关，因为那是最初隔离的国家。