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Identification of Japanese isolates of spotted fever group rickettsiae

斑疹热群立克次体日本分离株的鉴定

基本信息

批准号：
63044102
负责人：
UCHIDA Takahiro
金额：
$ 2.56万
依托单位：
University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044102/
关键词：
Sptted Fever Group Rickettsia Rickettsia japonica Spotted Fever リケッチア感染症

项目摘要

Spotted fever group (SFG) rickettsiae isolated from patients in the southeastern part of Shikoku, Japan, were analyzed to identify the species by use of mouse antisera. Antigenic differences between five strains of Japanese isolates and standard strains of pathogenic SFG rickettsiae were examined by calculating the specificity difference (SPD). the SPD between each pair of Japanese strains showed their antigenic identity, while the SPD between Japanese strains and pathogenic standard strains of SFG indicated that Japanese strains are clearly distinct from R.akari, R.australis, R.conorii, R.rickettsii, and R.sibirica. It was also demonstrated that Japanese strains are different from Thai tick typhus rickettsia (strain TT-118) isolated from a tick, known only in the Orient. Monoclonal antibody analysis resulted in no cross-reaction between Japanese strain and standard strains but positive reactions of Japanese strains with monoclonal antibody to Japanese strain. Comparison of the Japanese strain and standard strains of SFG rickettsiae by Western immunoblotting with mouse antiserum to Japanese strain demonstrated that the Japanese strain are distinct from any of the standard strain of SFG rickettsiae. The results obtained in this study demonstrated that the Japanese isolates of SFG rickettsiae belong to a single species distinct from any SFG rickettsiae known to be pathogenic to humans. We proposed the taxonomic name Rickettsia japonica sp. nov. pertaining to Japan, since that is the country in which it was first isolated.

使用小鼠抗血清对从日本四国东南部患者分离的斑疹热组（SFG）立克次体进行分析以鉴定其种类。通过计算特异性差异（SPD）来检查五株日本分离株和致病性 SFG 立克次体标准株之间的抗原差异。每对日本菌株之间的SPD显示了它们的抗原同一性，而日本菌株与SFG致病标准菌株之间的SPD表明日本菌株与R.akari、R.australis、R.conorii、R.rickettsii和R.sibirica明显不同。研究还表明，日本菌株与从蜱中分离出的泰国蜱斑疹伤寒立克次体（TT-118菌株）不同，后者仅在东方存在。单克隆抗体分析结果显示，日本株与标准株无交叉反应，但日本株与日本株单克隆抗体呈阳性反应。通过使用针对日本株的小鼠抗血清进行蛋白质免疫印迹来比较SFG立克次体的日本株和标准株，证明日本株与SFG立克次体的任何标准株都不同。本研究获得的结果表明，SFG 立克次体的日本分离株属于单一物种，与已知对人类致病的任何 SFG 立克次体不同。我们提出了分类学名称 Rickettsia japonica sp。十一月与日本有关，因为那是它首先被孤立的国家。