The identification of transcription factors which induce the secretion of neurotrophic factores in choroid plexus epithelial cells.-Aming at the application to the therapy for cerebral infarction by gene delivery of the transcription factor

诱导脉络丛上皮细胞分泌神经营养因子的转录因子的鉴定--转录因子基因递送在脑梗死治疗中的应用

• 批准号: 17590162
• 负责人: MATSUMOTO Naoya
• 金额: $ 2.24万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590162/
• 关键词: choroid plexus; tropohic factors; ischemic brain injury; rat; regeneration; neuroprotection; cerebrospinal fluid; transplantation; 転写因子; 脳梗塞

The choroid plexus (CP) provides a proper environment for the brain by supplying cerebrospinal fluid (CSF). The physiological function of CP in the brain, however, has not been uncovered yet. The infusion of cultured choroid plexus epithelial cells (CPECs) into CSF through the 4th ventricle against rat middle cerebral artery occlusion model protected the infarct expansion, suppressing apoptosis, inflammation and oxidative stress. The transplanted cells remained inside the ventricle and could not been found in the ischemic foci. This fact suggested that a variety of secreted factors derived from transplanted cells exerted trophic effects on neuroprotection via CSF. This speculation was supported by a study of the non-contact coculture system between CPECs and neurons or endothelial cells using transwell. The presence of CPECs a) promoted neurite extension, b) suppressed neuronal cell death, and c) augmented survival of endothelial cells. Furthermore, it was demonstrated that a part of neurotorphic or regenerative factors secreted from CPECs were involved in proteins/peptides which seize was more than 50kD. CP may play a crucial role in functional maintenance of the brain by regulating the microenvironmet of the central nervous system, by supplying a various trophic factors into CSF. The enhancement of secretagogue-induced transcriptional factors in CPECs against ischemic brain injury would promote the neuroprotection and neurogenesis, adjusting the CSF adverse environment, and become a new promising strategy.

脉络丛（CP）通过供应脑脊液（CSF）为大脑提供适当的环境。然而，CP在脑中的生理功能尚未被揭示。经第四脑室向大鼠脑脊液中注入培养的脉络丛上皮细胞（CPECs），对大鼠大脑中动脉闭塞模型具有保护脑梗死范围扩大、抑制细胞凋亡、炎症和氧化应激的作用。移植的细胞保留在心室内，在缺血灶内未发现。这一事实表明，多种来源于移植细胞的分泌因子通过CSF对神经保护发挥营养作用。使用transwell对CPECs与神经元或内皮细胞之间的非接触共培养系统的研究支持了这一推测。CPEC的存在a）促进神经突延伸，B）抑制神经元细胞死亡，和c）增加内皮细胞的存活。此外，还证明CPEC分泌的部分神经营养或再生因子涉及分子量大于50 kD的蛋白质/肽。CP可能通过调节中枢神经系统的微循环，向CSF中提供各种营养因子，在脑功能维持中起重要作用。增强促分泌素诱导的转录因子在CPECs抗缺血性脑损伤中的作用，将促进神经保护和神经再生，调节CSF不良环境，成为一种新的有前途的策略。

项目成果

Survival of neural progenitor cells from the subventricular zone of the adult rat after transplantation into the host spinal cord of the same strain of adult rat

• DOI: 10.1111/j.1447-073x.2005.00128.x
• 发表时间: 2005-12-01
• 期刊: Anatomical Science International
• 影响因子: 1.2
• 作者: Mligiliche, Nurru Lameck;Xu, Yi;Ide, Chizuka
• 通讯作者: Ide, Chizuka

Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons

• DOI: 10.1016/j.neulet.2004.12.068
• 发表时间: 2005-05
• 期刊: Neuroscience Letters
• 影响因子: 2.5
• 作者: Yumi Watanabe;N. Matsumoto;M. Dezawa;Yutaka Itokazu;T. Yoshihara;C. Idé

Survival of neural progenitor cells from the subventricular zone of adult rat

成年大鼠室下区神经祖细胞的存活

• 发表时间: 2005
• 期刊: Anat.Sci.Int. 80
• 作者: Mligilichi N.L.;Xu Y.;Matsumoto N.;et. al.
• 通讯作者: et. al.

Chapter 21- Grafting of the choroid plexus : clinical implications.

第21章-脉络丛移植：临床意义。

• 发表时间: 2005
• 期刊: The blood-cerebrospinal fluid barrier
• 作者: Matsumoto;N.;Itokazu;Y.;Kitada;M.;et al.
• 通讯作者: et al.

Grafting of the choroid plexus:clinical implication

脉络丛移植：临床意义

• 发表时间: 2005
• 期刊: The blood-cerebrospinal fluid barrier Chapter 21
• 作者: Matsumoto N.;et. al.
• 通讯作者: et. al.