Effect of docosahexaenoic acid on the differentiation of neural steam cells
二十二碳六烯酸对神经蒸汽细胞分化的影响
基本信息
- 批准号:17590205
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Docosahexaaenoic acid (DHA), one of the main structural lipids in the mammalian brain, plays crucial roles in the development and function of brain neurons. We previously reported that chronic administration of DHA enhances long-term memory in young and aged rats, and dietary DHA has preventive and ameliorative effects on the impairment of spatial cognition learning ability in amyloid b-infused rats. These findings suggest that DHA plays crucial roles in the development and function of the CNS throughout life. In this study, we examined the 1) effect of DHA on neural differentiation of neural stem cells (NSCs) in vivo and in vitro, and 2) on the expression of basic helix-loop-helix (bHLH) transcriptional factors of the cultured cells.<Methods> NSCs obtained from 15.5-day-old rat embryos were propagated as neurospheres and cultured under differential conditions with or without DHA. Total RNA was prepared from each culture with or without DHA, and was performed RT-PCR (Hes1, Hes5, neurog … More enin1, Mash1 or NeuroD).<Results and Conclusions> 1) DHA significantly increased the number of Tuj 1-positive neurons compared with the control on cultured NSCs, and the newborn neurons in the DHA groups were morphologically more mature than in the control, DHA significantly decreased the incorporation ratio of 5-bromo-2-deoxyuridine, the mitotic division marker, during the first 24h periods. Furthermore, dietary administration of DHA significantly increased the number of 5-bromo-2-deoxyuridine(+)/NeuN(+) newborn neurons in the granule cell layer of the dentate gyrus in adult rats. These results suggested that DHA effectively promotes nuerogenesis both in vitro and in vivo.2) Treatment of NSCs with DHA decreased the expression of Hes1 mRNA compared with that of control, suggesting that DHA stimulates neuronal differentiation by modulating expression levels of bHLH transcription factors.The present study suggests that DHA has the new property of modulating hippocampal function regulated by neurogenesis. Less
二十二碳六烯酸(DHA)是哺乳动物脑内的主要结构脂质之一,对脑神经元的发育和功能起着至关重要的作用。我们以前报道过,长期给予DHA可增强年轻和老年大鼠的长期记忆,膳食DHA对淀粉样蛋白b注入大鼠的空间认知学习能力障碍具有预防和改善作用。这些发现表明,DHA在整个生命过程中对CNS的发育和功能起着至关重要的作用。在这项研究中,我们研究了1)DHA在体内和体外对神经干细胞(NSCs)神经分化的影响,以及2)对培养细胞的碱性螺旋-环-螺旋(bHLH)转录因子表达的影响。<Methods>从15.5日龄大鼠胚胎中获得的神经干细胞作为神经球繁殖,并在有或无DHA的不同条件下培养。从含有或不含DHA的每种培养物中制备总RNA,并进行RT-PCR(Hes 1,Hes 5,neurog ...更多信息 enin 1、Mash 1或NeuroD)。<Results and Conclusions>1)与对照组相比,DHA能显著增加培养的NSCs上Tuj 1阳性神经元的数量,且DHA组新生神经元的形态较对照组成熟,DHA能显著降低有丝分裂标记物5-溴-2-脱氧尿苷的掺入率。此外,饮食中给予DHA显著增加了成年大鼠齿状回颗粒细胞层中5-溴-2-脱氧尿苷(+)/NeuN(+)新生神经元的数量。2)与对照组相比,DHA处理NSCs后Hes 1 mRNA表达降低,提示DHA通过调节bHLH转录因子的表达水平促进神经元分化,提示DHA具有调节海马功能的新特性。少
项目成果
期刊论文数量(52)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nobiletin restoring β-amyloid-impaired CREB phosphorylation rescues memory deterioration in Alzheimer's disease model rats
- DOI:10.1016/j.neulet.2006.02.077
- 发表时间:2006-06-12
- 期刊:
- 影响因子:2.5
- 作者:Matsuzaki, Kentaro;Yamakuni, Tohru;Ohizumi, Asushi
- 通讯作者:Ohizumi, Asushi
Contribution of the suprachiasmatic nucleus to the formation of a time memory for heat exposure in rats.
视交叉上核对大鼠热暴露时间记忆形成的贡献。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:Shido O;et al.;Maruyama M et al.
- 通讯作者:Maruyama M et al.
Docosahexaenoic acid-induced amelioration on impairment of memory learning in amyloid β-infused rats relates to the decreases of amyloid β and cholesterol levels in detergent-insoluble membrane fractions
- DOI:10.1016/j.bbalip.2005.11.011
- 发表时间:2005-12-30
- 期刊:
- 影响因子:4.8
- 作者:Hashimoto, M;Hossain, S;Shido, O
- 通讯作者:Shido, O
Myosin light chain kinase and Rho-kinase participate in P2Y receptor-mediated acceleration of permeability through the endothelial cell layer.
肌球蛋白轻链激酶和 Rho 激酶参与 P2Y 受体介导的内皮细胞层通透性加速。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Naoko Tanaka;et al.
- 通讯作者:et al.
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HASHIMOTO Michio其他文献
Long-Term Ultra-High Hydrostatic Pressurized Brown Rice Intake Prevents Bone Mineral Density Decline in Elderly Japanese Individuals
长期摄入超高静压糙米可防止日本老年人骨矿物质密度下降
- DOI:
10.3177/jnsv.65.s88 - 发表时间:
2019 - 期刊:
- 影响因子:1.6
- 作者:
MATSUZAKI Kentaro;YANO Shozo;SUMIYOSHI Eri;SHIDO Osamu;KATSUBE Takuya;TABATA Mitsumasa;OKUDA Michiaki;SUGIMOTO Hachiro;YOSHINO Katsumi;HASHIMOTO Michio - 通讯作者:
HASHIMOTO Michio
HASHIMOTO Michio的其他文献
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{{ truncateString('HASHIMOTO Michio', 18)}}的其他基金
Multilateral verification of preventive effects of polyunsaturated fatty acids on dementia
多不饱和脂肪酸对痴呆症预防作用的多边验证
- 批准号:
23500955 - 财政年份:2011
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effects of fatty acids on differentiation of neuronal stem cells and the protective and ameliorative effects on dementia.
脂肪酸对神经元干细胞分化的影响以及对痴呆症的保护和改善作用。
- 批准号:
19500324 - 财政年份:2007
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of the software for patient safety education
患者安全教育软件开发
- 批准号:
15390167 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Relationship between changes in response to mechanoreceptor stimulation of endocardial endothelial cells and the development of heart failure
心内膜内皮细胞机械感受器刺激反应的变化与心力衰竭发展的关系
- 批准号:
10670043 - 财政年份:1998
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relationship between age-related changes in functions of endocardial endothelial cells and heart failure
心内膜内皮细胞功能年龄相关变化与心力衰竭的关系
- 批准号:
08838014 - 财政年份:1996
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Agr-related changes in vascular endothelial cell functions
Agr相关的血管内皮细胞功能变化
- 批准号:
04836016 - 财政年份:1992
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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Perinatal nutrition strategy with omega-3 docosahexaenoic acid to protect brain in fetal alcohol spectrum disorders
使用 omega-3 二十二碳六烯酸保护胎儿酒精谱系障碍大脑的围产期营养策略
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486469 - 财政年份:2022
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A novel method for elucidating sex-specific differences in docosahexaenoic acid synthesis rates of young women and men
一种阐明年轻女性和男性二十二碳六烯酸合成率性别特异性差异的新方法
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二十二碳六烯酸负载低密度脂蛋白:肝细胞癌的新型生物干预措施。
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10607845 - 财政年份:2022
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Effects of Docosahexaenoic Acid on Oxidative Stress White Matter Injury and Brain Inflammation in a Pre-Clinical Model of Pediatric TBI
二十二碳六烯酸对儿科 TBI 临床前模型中氧化应激白质损伤和脑炎症的影响
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Characterizing the roles of omega-3 fatty acids, eicosapentaenoic and docosahexaenoic acid, in isolation on skeletal muscle mass, size and strength in loading and unloading conditions in young women
单独描述 omega-3 脂肪酸、二十碳五烯酸和二十二碳六烯酸对年轻女性在加载和卸载条件下骨骼肌质量、大小和强度的作用
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