Agr-related changes in vascular endothelial cell functions

Agr相关的血管内皮细胞功能变化

• 批准号: 04836016
• 负责人: HASHIMOTO Michio
• 金额: $ 1.34万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04836016/
• 关键词: Aging; Vascular endothelial cells; Rat; Prostanoid; Cell migration; Purine compounds; NO; Vascular responses; プロスタノイド; 一酸化窒素; 内皮細胞由来血管他緩・収縮因子; 培養細胞

Age-related changes in vascular endothelial cell functions were estimated using young (5 weeks old), adult(40-50 weeks old)and old(100-110 weeks old)rats.Results and Conclusion1. Isolation of cultured endothelial cells(ECs) : From thoracic aorate and cadual arteries of young and old rats, endothelial cells were isolated by an explant method and could be cultured at least until the 20th passage.2. Cell proliferation and migration : A new and simple method for measureing the migration of ECs was developed. Both the incorporation of ^3H-thymidine into ECs and migration of the cells decreased with rat aging.3. Platelet aggregation and platelet / aortic prostanoid production : ADP-induced platelet aggregation increased with rat aging. TXA_2 levels of platelet and aortic / tha aortic ECs increased with aging, inducing increment in an index of platelet aggregation(platelet TXA_2 concentration / aortic PGI_2 production)with rat aging.4. Production of endothelium-derived contracting and relaxin … More g factors : Endotheline-1 secretion from caudal ECs increased with rat aging, while the endothelium-dependent relaxing response to acetylcholine and release of purine compounds from the caudal artery or the ECs decreased. These increments and decrements would induce an increase in blood systolic pressure with rat aging.5. Development of nitric oxide(NO)electrode : By collaborating with Intermedical Co.Ltd., a NO electrode was developed and the presence of 10^<-8>M NO, which is released by treatment of aortae with carbachol, could be detected by using the electrode.The measurement of the physiological and pathophysiological functions of rat vascular endothelial cells which were isolated by the explant method was confirmed to be a useful methods to elucidate the mechanisms of occurrence of vascular disease associated with human aging. From the present study, close relationships were also confirmed to exist between the occurrence of vascular disease and age-related dysfunction of endothelial cells. Less

采用青年（5周龄）、成年（40-50周龄）和老年（100-110周龄）大鼠，观察了高脂饮食对血管内皮细胞功能的影响。培养的内皮细胞的分离：采用组织块法从青年和老年大鼠胸主动脉和尸体动脉中分离内皮细胞，培养至20周.细胞增殖和迁移：建立了一种新的、简便的测定内皮细胞迁移的方法。随着年龄的增长，内皮细胞的^3H-胸苷掺入量和迁移能力均下降。血小板聚集和血小板/主动脉前列腺素生成：ADP诱导的血小板聚集随着大鼠年龄的增长而增加。血小板和主动脉/主动脉内皮细胞TXA_2水平随增龄而增加，血小板聚集指数（血小板TXA_2浓度/主动脉PGI_2生成）随增龄而增加.内皮源性收缩和松弛素的产生 ...更多信息 g因素：随着年龄的增长，尾动脉内皮细胞分泌内皮素-1的能力增强，而尾动脉或内皮细胞对乙酰胆碱的内皮依赖性舒张反应和嘌呤类化合物的释放能力下降。随着年龄的增长，这些增加和减少都会引起收缩压的升高.一氧化氮（NO）电极的开发：通过与Intermedical有限公司合作，研制了一种NO电极，并<-8>利用该电极检测了卡巴胆碱处理大鼠动脉后释放的10 μ M NO，证实了用组织块法分离的大鼠血管内皮细胞的生理和病理生理功能的测定是一种阐明与人类衰老有关的血管疾病发生机制的有用方法。从本研究中，也证实了血管疾病的发生与年龄相关的内皮细胞功能障碍之间存在密切关系。少

项目成果

M.Hashimoto: "Metabolic aspects of endothelial cells cultured from rat aortae." Artery. 19(5). 284-296 (1992)

M.Hashimoto：“从大鼠主动脉培养的内皮细胞的代谢方面。”

M.Hashimoto: "Notes on the acetylcholine-induced relaxation of porcine coronary arteries." Life Science. 54. 525-531 (1994)

M.Hashimoto：“关于乙酰胆碱诱导的猪冠状动脉松弛的注释。”

M.Hashimoto, et al.: "Dependence of endothelium-mediated relaxation on oxygen and metabolism in porcine coronary arteries" American Jounal of Physiology. 265(Heart Circ.Physiol.34). H299-306 (1993)

M.Hashimoto 等人：“猪冠状动脉中内皮介导的舒张对氧和代谢的依赖性”美国生理学杂志。

M.Hashimoto: "Notes on the acetylcholine-induced relaxation of porcine coronary artery" Life Science. 54. 525-531 (1994)

M.Hashimoto：“关于乙酰胆碱诱导的猪冠状动脉松弛的注释”生命科学。

M.Hashimoto: "Dependence of endothelium-mediated relaxation on oxygen and metabolism in porcine coronary arteries." American Journal of Physiology. 265. H299-H306 (1993)

M.Hashimoto：“内皮介导的舒张对猪冠状动脉氧和代谢的依赖性。”