Pre-thymic Notch signaling is necessary for GATA3 function in the initiation of T cell development.

胸腺前 Notch 信号传导对于 T 细胞发育起始中的 GATA3 功能是必需的。

• 批准号: 17590441
• 负责人: HOZUMI Katsuto
• 金额: $ 2.24万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590441/
• 关键词: T cells; GATA3; Notch; Notchシグナル; 分化

GATA3 and Notch1 are essential for T cell fate at the earliest stage, but their mutual roles in this process remain to be clarified. In this study, we demonstrated that the impairment of T lymphopolesis in hematopoietic progenitor cells of GATA3-deficient fetal liver (FL) on 11.5 days of gestation (E11.5) was rescued only by introduction of both GATA3 and the intracellular region of Notch1 but not by either alone. However, the introduction of GATA3 only was sufficient for T cell induction in GATA3-deficient FL cells at the advanced stage, where Notch signaling is well detectable. This indicates that Notch signaling is necessary for GATA3 to function for the specification of T cell fate but is not sufficient without GATA3. On the other hand, Notch signaling is enough for the blockage of B cell development without GATA3, suggesting that specification of T cell fate at the branching point does not result simply from the developmental arrest of B cell lineage by Notch signaling.

GATA3和Notch1在T细胞的最早阶段是必不可少的，但它们在这一过程中的相互作用仍有待澄清。在这项研究中，我们证明了在妊娠11.5天（E11.5）的GATA 3缺陷胎肝（FL）造血祖细胞中T淋巴细胞减少的损伤仅通过引入GATA 3和Notch 1的细胞内区域来挽救，而不是单独使用。然而，仅引入GATA3足以在晚期阶段在GATA3缺陷型FL细胞中诱导T细胞，其中Notch信号传导是可良好检测的。这表明Notch信号传导对于GATA 3发挥T细胞命运的特异性功能是必需的，但在没有GATA 3的情况下是不够的。另一方面，Notch信号传导足以在没有GATA 3的情况下阻断B细胞发育，这表明T细胞在分支点的命运的特化不是简单地由Notch信号传导引起的B细胞谱系的发育停滞引起的。