Development of quantitative analyzing method for phase X-ray CT images of biological objects

生物体X射线CT图像定量分析方法的研制

• 批准号: 17591244
• 负责人: WU Jin
• 金额: $ 2.37万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591244/
• 关键词: Synchrotron radiation; X-ray interferometer; Phase-contrast X-ray CT; Biomedical imaging; Oncology; Alzheimer's disease; Kidney; Animal model of human disease; 生体イメージング; 血管; 癌; 小動物; 形態画像; 定量化

Currently, X-ray micro-imaging techniques for small laboratory animal models of human diseases are an exciting research topic. To obtain the fine morphological information, it is inevitable to take on the optimal scale of imaging modality on small animals, and high-contrast and high-spatial resolutions are required. X-ray phase-contrast imaging techniques with an X-ray interferometer, which has about 1000-times higher contrast sensitivity for the light atomic elements than conventional X-ray absorption-contrast method, enables us to enhance the contrast of biological soft tissue without a contrast agent. The aim of this study is to develop a three-dimensional quantitative analyzing method for the evaluation of the physiological states of small animals.The X-ray phase-contrast CT (PCCT) system consists of a triple Laue-case X-ray interferometer, a phase shifter, a sample cell and an X-ray CCD camera. The analyzer thickness was 1mm at the PF of the High Energy Accelerator Research Organi … More zation (KEK), and 0.04 mm at 20XU of SPring-8 respectively. The pixel size of the detector was 0.018mm x 0.018mm at PF of KEK, and 0.004 mm x 0.004 mm at SPring-8 respectively.The formalin-fixed biological samples of human diseases models, such as colon cancer implanted in nude mice, the brain with Alzheimer's disease in mice, and the kidney of focal segmental glomerulosclerosis in hamsters, are imaged. 3D PCCT images could clearly demonstrate various pathological changes and anatomical structures of colon cancer specimens without a contrast agent. In the case of Alzheimer's disease, the deposition of B amyloid was visualized in the cortex and the hippocampus, and its number and size of individual B amyloid plaque were analyzed quantitatively. In the kidney, glomeruli and tubular, their structures were observed as similar images to 40 or 100-time magnified optical microscope images by our micro-PCCT. The distribution of renal microvasculature, and the number and size of glomeruli were successfully depicted and observed. The density of renal tissue, and glomerular volume were also analyzed quantitatively. Our results suggest that the quantitative PCCT image analysis could provide important biomedical information for small animal research. Less

目前，X射线显微成像技术用于人类疾病的小型实验动物模型是一个令人兴奋的研究课题。为了获得精细的形态学信息，在小动物上采用最佳的成像模式是必然的，并且要求高对比度和高空间分辨率。X射线相位衬度成像技术与X射线干涉仪，它具有约1000倍的高对比灵敏度的轻原子元素比传统的X射线吸收对比度的方法，使我们能够提高生物软组织的对比度没有造影剂。本研究旨在建立一种用于小动物生理状态评价的三维定量分析方法，X射线相位衬度CT（PCCT）系统由三路劳厄干涉仪、移相器、样品池和X射线CCD相机组成。在高能加速器研究机构的PF处，分析器厚度为1 mm。 ...更多信息 zation（KEK）和SPring-8在20 XU下的0.04mm。探测器的像素尺寸分别为0.018mm × 0.018mm和0.004mm × 0.004mm。对人结肠癌裸鼠移植瘤、Alzheimer病小鼠脑组织和仓鼠局灶节段性肾小球硬化症肾脏等疾病模型的福尔马林固定生物样品进行了成像。在无造影剂的情况下，三维PCCT图像可清晰显示结肠癌标本的各种病理变化和解剖结构。在阿尔茨海默病的情况下，B淀粉样蛋白的沉积在皮质和海马中可见，并且定量分析单个B淀粉样蛋白斑块的数量和大小。在肾脏、肾小球和肾小管中，它们的结构通过我们的micro-PCCT观察到与放大40或100倍的光学显微镜图像相似的图像。成功地描绘和观察了肾微血管的分布、肾小球的数量和大小。并对肾组织密度、肾小球体积进行定量分析。我们的研究结果表明，定量PCCT图像分析可以为小动物研究提供重要的生物医学信息。少

项目成果

Phase-contrast and fluorescent X-ray imaging for biomedical researches

• DOI: 10.1016/j.nima.2005.03.063
• 发表时间: 2005-08
• 期刊: Nuclear Instruments & Methods in Physics Research Section A-accelerators Spectrometers Detectors and Associated Equipment
• 影响因子: 1.4
• 作者: T. Takeda

validity of fusion imaging of hamster heart obtained by nuorescent and phase-contrast X-ray CT with synchrotron radiation.

通过同步辐射的荧光和相差 X 射线 CT 获得的仓鼠心脏融合成像的有效性。

• 发表时间: 2007
• 期刊: Proc.AIP CP 879
• 作者: Takeda T;et al.;松本 直起 他;Jin Wu
• 通讯作者: Jin Wu

Fusion imaging of fluorescent and phase-contrast x-ray computed tomography using synchrotron radiation in medical biology

• DOI: 10.1117/12.680133
• 发表时间: 2006-08
• 期刊: Nature
• 影响因子: 64.8
• 作者: Jin Wu;T. Takeda;T. Lwin;N. Sunaguchi;T. Fukami;T. Yuasa;M. Minami;T. Akatsuka

Quantitative analysis of amyloid plaques in a mouse model of alzheimer's desease by phase-contrast X-ray computed tomography.

通过相差 X 射线计算机断层扫描对阿尔茨海默病小鼠模型中的淀粉样斑块进行定量分析。

• 发表时间: 2006
• 期刊: Neuroscience 138
• 作者: Tsuboi K;Matsuda W;Nakamura K;Takano S;Kyoko Noda-Saita
• 通讯作者: Kyoko Noda-Saita

Quantitative analysis of amyloid plaques in a mouse model of Alzheimer's disease by phase-contrast X-ray computed tomography

• DOI: 10.1016/j.neuroscience.2005.12.036
• 发表时间: 2006-01-01
• 期刊: NEUROSCIENCE
• 影响因子: 3.3
• 作者: Noda-Saita, K;Yoneyama, A;Okada, M
• 通讯作者: Okada, M