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Visualization of Molecular Mechanism of Brain Function and Neurological Disease Using High Magnetic Field MRI

使用高磁场 MRI 可视化脑功能和神经系统疾病的分子机制

基本信息

批准号：
17591275
负责人：
HARADA Masafumi
金额：
$ 2.5万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591275/
关键词：
MRS GABA NAA MEGA-PRESS SPECT 3T MRI Glutamate neurotrasmitter MR spectroscopy

项目摘要

Gamma-aminobutyric acid (GABA) in normal subjects and patients was measured using MEGA-PRESS on clinical 3T MR apparatus and the GABAnergic index was proposed. The result of GABA nergic index was compared with the data of receptor scintigraphy in the autistic patients.Ten autistic patients (2〜11 y.o., mean 5.2 y.o.) were measured by routine anatomical MRI and after obtaining informed consents from their parents, single voxel proton MRS including MEGA-PRESS was conducted. As age-matched normal controls, six children (3〜12 y.o., mean 6.6 y.o.) who show no neurological abnormality and anatomical disorder in the brain by MRI were recruited after getting informed consents. The measured locations were left frontal lobe and left basal ganglia. The in-vitro data of NAA, Glutamine, Glutamate and GABA were acquired by MEGA-PRESS and set as a basis-set for LCModel. The quantification of signals in the difference and original spectrum by MEGA-PRESS were conducted by LCModel. A new parameter for GABAnergic neurons was defined as followed: GABAnergic index=(GABA signal from a subtracted spectra divided by NAA signal from the original spectra without selective pulses).The result of GABAnergic index in the frontal lobe and basal ganglia and this index in the frontal lobe on autistic patients were lower than that on normal controls (p<0.05). However, there is no difference in the basal ganglia between autism and normal controls. An example of the receptor scintigraphy (iomazenil SPECT) showed the activity ratio of the frontal lobe/cerebellum in autism was lower than that of the normal controls. This finding on the receptor scintigraphy was almost consistent with the result of GABAnergic index, suggesting the decrease of GABAnergic activity in the frontal lobe on autistic patients.The GABAnergic index calculated from MEGA-PRESS data would be useful for the evaluation of neurochemical disorder on autistic patients.

使用MEGA-PRESS在临床3T MR设备上测量正常受试者和患者的γ-氨基丁酸（GABA），并提出GABA能指数。将自闭症患者的GABA能指数结果与受体显像数据进行比较。对10例自闭症患者（2～11岁，平均5.2岁）进行常规解剖MRI测量，在征得家长知情同意后，进行包括MEGA-PRESS在内的单体素质子MRS。作为年龄匹配的正常对照，在知情同意后招募 6 名 MRI 未显示脑部神经系统异常和解剖学异常的儿童（3〜12 岁，平均 6.6 岁）。测量的位置是左额叶和左基底神经节。 NAA、谷氨酰胺、谷氨酸和GABA的体外数据通过MEGA-PRESS获取并设置为LC模型的基础集。 MEGA-PRESS 对差异谱和原始谱中信号的量化是通过 LCModel 进行的。 GABA能神经元的新参数定义如下：GABA能指数=（减去光谱的GABA信号除以无选择性脉冲的原始光谱的NAA信号）。自闭症患者额叶和基底节的GABA能指数结果以及额叶的该指数低于正常对照（p<0.05）。然而，自闭症患者和正常对照者的基底神经节没有差异。受体闪烁扫描（碘马西尼 SPECT）的一个例子显示，自闭症患者额叶/小脑的活动比率低于正常对照。受体闪烁扫描的这一发现与GABA能指数的结果几乎一致，表明自闭症患者额叶GABA能活性降低。根据MEGA-PRESS数据计算的GABA能指数将有助于评估自闭症患者的神经化学紊乱。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

MR spectroscopy

磁共振波谱

DOI：
发表时间：
2006
期刊：
Clinical Neuroscience 24・4
影响因子：
0
作者：
Sumitani S;Harada M;et al.;Hattori Y;Sumitani S;Ando H;Maeda N;原田雅史;Morita N;原田雅史;原田雅史;原田雅史
通讯作者：
原田雅史

脳神経外科学大系第2巻検査・診断法MRS

神经外科第 2 卷测试和诊断方法 MRS