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Evaluation of Changes of Environment in Intra- and Extra-cellular Compartments using Metabolite Diffusion

利用代谢物扩散评估细胞内和细胞外环境的变化

基本信息

批准号：
13670944
负责人：
HARADA Masafumi
金额：
$ 2.24万
依托单位：
UNIVERSITY OF TOKUSHIMA
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670944/
关键词：
MRS diffusion LCModel metabolite cerebral infarction brain tumor NAA lactate Proton MRS 拡散コンパートメント

项目摘要

The apparent diffusion coefficients (ADCs) of water and metabolites observed by proton MR spectroscopy were evaluated in healthy volunteers and pathological changes in cases of acute cerebral infarction and brain tumor using a clinical MR scanner. The ADCs of NAA and Cr in cases of acute infarcion who were measured within 24 hours from onset were decreased compared to those of the normal controls. The ADC of Cho in cases of acute infarction was not much changed compared to NAA and Cr. This result suggested that the environment of Cho content was different from those of NAA and Cr.The ADCs of metabolits in tumors were increased relative to those the controls, espeialy that of NAA much increased. The ADC of Cho in tumors was also increased more than that in acute infarction. This result indicated that different change of enviroment around Cho content existed between tumors and acute infarction.These findings suggest that neuronal cell viscosity changes according to pathological status because NAA is a neuronal marker. Lactate ADC increased relative to the values of the other three major metabolites in cases of ischemia and tumor, suggesting their existence in a different compartment. These results indicate that metabolaite diffusion can be used to reveal differences in the intracellular environment depending on the pathological status.

应用质子磁共振波谱技术，对健康志愿者和急性脑梗死、脑肿瘤患者的脑组织进行了水和代谢物的表观扩散系数（ADC）测定。发病24小时内的急性脑梗死患者NAA和Cr的ADC值较正常对照组降低。急性脑梗死患者Cho的ADC值与NAA、Cr相比无明显变化。结果表明，Cho含量的环境与NAA、Cr不同，肿瘤代谢产物的ADC值较对照组增高，尤以NAA增高幅度最大。Cho在肿瘤中的ADC值也比急性脑梗死高。结果表明，肿瘤与急性脑梗死时Cho含量周围环境的变化不同，提示NAA是神经元的标志物，神经元细胞粘度的变化与病理状态有关。在缺血和肿瘤的情况下，乳酸盐ADC相对于其他三种主要代谢物的值增加，表明它们存在于不同的隔室中。这些结果表明，代谢物扩散可用于揭示细胞内环境的差异，这取决于病理状态。