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Basic study for the improvement of melanoma radiotherapy by suppression of TRP-2 gene expression

通过抑制TRP-2基因表达改善黑色素瘤放疗的基础研究

基本信息

批准号：
17591301
负责人：
KONDOH Hirofumi
金额：
$ 2.18万
依托单位：
Kobe Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591301/
关键词：
Radiation X-ray Particle-beam Gene Cancer Control of gene expression Resistance Therapy

项目摘要

TRP-2 gene is specifically expressed in melanoma cells, and is thought to related to the radio-resistance of melanoma. The aim of this project is to establish the method of improvement of radiotherapy for melanoma by suppression of TRP-2 gene expression, cultured melanoma cell level experiments were performed as the first step.The results obtained through this research project are summarized as follows :1. TRP-2 sh-RNA expression plasmids were transfected to tyrosinase-ecpression deficient amelanotic melanoma cell to construct TRP-2 expression deficient melanoma cell lines.2. Evaluation systems for the killing effect of X-ray, UV, and cisplatin were established to the effect of TRP-2 expression in melanoma cells.3. Evaluation system for measuring the content of melanin monomer DHICA, which is the product of TRP-2, was established.4. Inversely, to evaluate the effect of TRP-2 expression in cells, non-melanoma cultured cell, HeLa cells were transfected with TRP-2 expression plasmid. Using this cell line we can clarify whether the radiation-resistance of melanoma is related to the expression and production of TRP-2 protein or melanogenesis related to TRP-2. We evaluated the properties of these cell lines concerning melanogenesis, cell biology, and molecular biology.Comparing the these properties of established cell lines, we could not obtained clear results that TRP-2 gene suppression improve the killing effect of X-ray radiation, UV, and cisplatin. Currently, we improve the evaluation systems and methods, and continue to the evaluation of the effects.

TRP-2基因在黑色素瘤细胞中特异表达，被认为与黑色素瘤的放射抗性有关。本项目的目的是建立通过抑制TRP-2基因表达来改善黑色素瘤放疗的方法，第一步进行了培养黑色素瘤细胞水平的实验。通过本研究项目获得的结果总结如下： 1．将TRP-2 sh-RNA表达质粒转染酪氨酸酶抑制缺陷型无黑色素瘤细胞，构建TRP-2表达缺陷型黑色素瘤细胞系。 2．建立了X射线、紫外线和顺铂对黑色素瘤细胞TRP-2表达影响的评价体系。 3．建立了TRP-2产物黑色素单体DHICA含量测定评价体系。 4．相反，为了评价TRP-2表达在细胞中的效果，将TRP-2表达质粒转染非黑色素瘤培养细胞、HeLa细胞。利用该细胞系，我们可以阐明黑色素瘤的放射抗性是否与TRP-2蛋白的表达和产生有关，或者与TRP-2相关的黑色素生成有关。我们评估了这些细胞系在黑素生成、细胞生物学和分子生物学方面的特性。比较已建立的细胞系的这些特性，我们无法获得TRP-2基因抑制提高X射线辐射、紫外线和顺铂的杀伤效果的明确结果。目前，我们正在完善评估体系和方法，持续评估效果。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Synthesis and evaluation as MRI probe of the trifluoromethylatedp-boronophenylalanine and its alcohol.

三氟甲基化对硼苯丙氨酸及其醇的合成和MRI探针评价。

DOI：
发表时间：
2007
期刊：
Bioorg Med Chem 15・5
影响因子：
0
作者：
Kakinuma S;Kodama Y;Amasaki Y;Yi S;Tokairin Y;Arai M;Nishimura M;Monobe;M;Kojima S;Shimada Y.;Hattori Y
通讯作者：
Hattori Y

Palliative radiotherapy for metastatic bone tumor.

转移性骨肿瘤的姑息放射治疗。