Analysis of expression mode of BMP in positional information in jaw regeneration or Ng

颌骨再生或Ng位置信息中BMP的表达模式分析

• 批准号: 17592105
• 负责人: YAGAMI Kimitoshi
• 金额: $ 2.3万
• 依托单位: SHOWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592105/
• 关键词: Tissue regeneration; Positional information; BMP2; BMP4; Noggin; Osteogenesis; Chondrogenesis; Concentration gradient; BMP; BMP-4; noggin; 骨芽細胞; 軟骨細胞; 遺伝子

Objective : The differentiation of mesenchymal stem cells (MSCs) into chondrocytes and osteoblasts is minutely regulated by bone morphogenetic proteins (BMPs) and various factors. There is no study describing the role of noggin in the differentiation from MSCs to chondrocytes or osteoblasts. We thus examined the effect of noggin on chondrogenesis and osteogenesis in a human cell line that expresses chondrocytic phenotypes, and the role of noggin in these processes. Noggin induces chondrogenic differentiation, and its repression induces osteogenic differentiation through BMP effects. Noggin is reported to bind several BMPs and to inhibit their functions, but its exact role is not yet clarified. USAC cells usually show chondrogenic phenotypes and also have the potential to differentiate into osteoblasts and adipocytes under some circumstances. Therefore, we investigated the effects of noggin on chondrogenic and osteogenic differentiation of USAC cells in vitro and in vivo.Materials and M … More ethods : After USAC cells were treated with noggin antisense oligonucleotide (As-Noggin) or noggin protein (rhNoggin), RT-PCR was performed to detect mRNAs of type I collagen (Col I), type II collagen (Col II), Cbfa1, aggrecan (AG), Sox9 and osteocalcin (OC) on days 3, 7 and 14. Western blot analysis was done to detect Col II, BMP-2, BMP-4, Cbfa1 and OC proteins. Toluidine blue staining and immunostaining for Col II, BMP-2, Cbfa1 and OC were also performed:Results and Conclusions : As-Noggin induced the up-regulation of Col I and OC mRNA expression in USAC cells time-dependently, while it down-regulated the expression of Col II and AG mRNAs. As-Noggin stimulated BMP-2, Cbfa1 and OC production, but it reduced the production of Col II and BMP-4. Moreover, OC mRNA expression and BMP-2 production were down-regulated by the addition of rhNoggin. The same results were obtained by immunostaining. These results suggest that noggin may regulate chondrogenic and osteogenic differentiation through the production of BMP-2 and BMP-4. Less

目的：骨髓间充质干细胞（mesenchymal stem cells，MSCs）向软骨细胞和成骨细胞的分化受骨形态发生蛋白（bone morphogenetic proteins，BMPs）和多种因素的调控。没有研究描述noggin在从MSC分化为软骨细胞或成骨细胞中的作用。因此，我们研究了noggin对表达软骨细胞表型的人类细胞系中软骨形成和骨形成的影响，以及noggin在这些过程中的作用。头蛋白诱导软骨形成分化，并且其抑制通过BMP作用诱导成骨分化。据报道，Noggin结合几种BMP并抑制其功能，但其确切作用尚未阐明。USAC细胞通常表现出软骨形成表型，并且在某些情况下也具有分化成成骨细胞和脂肪细胞的潜力。因此，我们研究了noggin在体外和体内对USAC细胞成软骨和成骨分化的影响。 ...更多信息 方法：分别用反义寡核苷酸（As-Noggin）或头蛋白（rhNoggin）处理USAC细胞，于第3、7、14天用RT-PCR检测I型胶原（Col I）、II型胶原（Col II）、Cbfa 1、聚集蛋白聚糖（AG）、Sox 9和骨钙素（OC）的mRNA表达。Western blot检测Col II、BMP-2、BMP-4、Cbfa 1和OC蛋白的表达。结果与结论：As-Noggin可诱导USAC细胞Col I和OC mRNA表达上调，Col II和AG mRNA表达下调，并呈时间依赖性。As-Noggin刺激BMP-2、Cbfa 1和OC的产生，但减少Col II和BMP-4的产生。此外，rhNoggin的加入还下调了OC mRNA的表达和BMP-2的产生。免疫组化染色也得到了同样的结果。这些结果表明，头蛋白可能通过产生BMP-2和BMP-4来调节软骨和成骨分化。少

项目成果

TITLE OF ARTICLE : Noggin prevents osteogenesis but induces chondrogenesis in a human mesenchymal cell line

文章标题：Noggin 可防止人间充质细胞系中的成骨，但诱导软骨形成

• 期刊: The Journal of Showa University Dental Society (In press)
• 作者: AUTHORS :Yasumasa Yoshizawa;Kimitoshi Yagami;Yohei Uyama;Saburo Kakuta;Masao Nagumo
• 通讯作者: Masao Nagumo

Noggin prevents osteogenesis but induces chondrogenesis in a human mesenchymal cell

Noggin 可防止人间充质细胞成骨，但诱导软骨形成

• 发表时间: 2007
• 期刊: 昭和歯学会誌
• 作者: Y.KADOMA;N.OKADA;吉澤泰昌
• 通讯作者: 吉澤泰昌

Noggin prevents osteogenesis but induces chondrogenesis in a human mesenchymal cell line(USAC)

Noggin 在人间充质细胞系 (USAC) 中阻止成骨，但诱导软骨形成

• 发表时间: 2007
• 期刊: 昭和歯学会誌 (6月掲載予定)
• 作者: Hanako OHKA Masaru KUDO;Noboru SHINYA.;吉澤泰昌
• 通讯作者: 吉澤泰昌