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Role of SOD in malignant tumor by anti cancer drugs

SOD抗癌药物在恶性肿瘤中的作用

基本信息

批准号：
06672020
负责人：
YAGAMI Kimitoshi
金额：
$ 1.22万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672020/
关键词：
Malignant tumor SOD activity radiation anti cancer drugs tumor cell line xanthin-xanthin oxidase progressed cancer sensitivity Xanthin-Oxidase Xanthin Oxidase

项目摘要

Recently, many anti cancer drugs have been shown to produce super oxide anion (O_2^-) and seem to involve O_2^- in their mode of action. Ionizing radiation provokes the decomposition reaction of water, producing a variety of reactive oxygen species including O_2^-. The finding that cancer cells are generally low in SOD activity may offer a theoretical base for radiation therapy and chemotherapy. Thus, intracellular SOD level may be an important factor determining the resistance of cancer cells against anti cancer drugs or radiation.For this purpose, we analyzes the activity of tumor tissue SOD that probed tumor sample form patient at the first time visit, and at the operation. Treatment with anti cancer drugs or radiation a few increasea of SOD activity in tumor tissue. To analyzes the mechanisms of resistance of cancer cells against anti cancer drugs or radiation, xanthin and xanthin oxidase were employed to some tumor cell line as an O_2^- generating system, and treatment with 5-FU or CDDP or ionizing radiation. Treatment with anti cancer drugs or radiation a few increases of SOD activity in tumor cells. The treatment with TNF or INF-gamma did a small reduction of SOD activity in tumor cells. CuZnSOD, catalase and GSH-px mRNAs were up regulated by O_2^- exposure with in 20 minus.

近年来，许多抗肿瘤药物被证明能产生超氧阴离子（O_2^-），并且在它们的作用方式中似乎涉及O_2^-。电离辐射引起水的分解反应，产生包括O_2^-在内的多种活性氧。肿瘤细胞普遍存在SOD活性低下的现象，为肿瘤的放射治疗和化疗提供了理论依据。因此，细胞内SOD水平可能是决定癌细胞对抗癌药物或放射线抵抗的重要因素，为此，我们对首次就诊患者和手术患者的肿瘤组织进行了SOD活性检测。抗癌药物治疗或放射治疗可使肿瘤组织中SOD活性少量升高.为探讨肿瘤细胞对抗癌药物和放射线的抵抗机制，本研究将黄嘌呤和黄嘌呤氧化酶作为O_2 ~-产生系统，应用于肿瘤细胞系，并以5-FU、CDDP或电离辐射进行治疗。抗肿瘤药物治疗或放射线治疗可使肿瘤细胞SOD活性少量升高.用TNF或INF-γ治疗肿瘤细胞中的SOD活性略有降低。在20 ℃时，O_2 ~+暴露使CuZnSOD、CAT和GSH-px mRNA表达上调。