THE ESTABLISHMENT OF ORAL CANCER THERAPY USING TISSUE SPECIFIC INF-r GENE

使用组织特异性 INF-r 基因建立口腔癌疗法

• 批准号: 10671906
• 负责人: YAGAMI Kimitoshi
• 金额: $ 2.11万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671906/
• 关键词: INF-r; Oral Cancer; B 16 melanoma; mouse; Invasion, metastasis; cytokine; B16メラノーマ細胞; アデイウイルスベクター; 遺伝子導入

1. The establishment of transplanted and metastasized mouse with carcinoma cell lineWe succeed establishment of the B 16 melanoma transplanted and metastasized mouse by using B 16 melanoma cells into C57/BL16 mouse subcutaneously and inter venously.2. Transformation of IFN-r gene expression vector into B 16 melanomaWe succeed transformation of IFN-r gene expression vector (pAdex1CAmINF-r) into B 16 melanoma cells in culture. INF-r was detected from pAdex1CAmINF-r transformed B 16 melanoma culture medium.The proliferation of the cells were not changed by the gene transformation. The production levels of INF-r were decreased after some passage.3. Transformation of IFN-r gene expression vector into HSC-4 cells or human oral squamous cell carcinoma (NA)We succeed transformation of IFN-r gene expression vector (pAdex1CAmINF-r) into HSC-4 cells or NA cells in culture. INF-r was detected from pAdex1CamINF-r transformed HSC-4 cells or NA cells culture medium.4. Characterization of pAdex1CAmINF-r transformed HSC-4 cells or human oral squamous cell carcinoma (NA)The proliferation of the cells were not changed by the gene transformation. The production levels of INF-r were decreased after some passage.5. The establishment of transplanted and metastasized mouse with pAdex1CAmINF-r transformed HSC-4 cells or NA cellsLevels of the transformation and transplantation were very low. We did not detect IFN-r from transplanted tumors.

1.肿瘤细胞系移植转移小鼠的建立将B 16黑色素瘤细胞皮下和静脉注射到C57/BL 16小鼠体内，成功建立了B 16黑色素瘤移植转移小鼠. IFN-γ基因表达载体对B 16黑色素瘤细胞的转化我们成功地将IFN-γ基因表达载体（pAdex 1 CAmINF-γ）转化到体外培养的B 16黑色素瘤细胞中。pAdex 1 CAmINF-r转化的B 16黑色素瘤细胞培养液中可检测到INF-r的表达，且基因转化对细胞增殖无影响。INF-r的产生水平在一段时间后下降。IFN-γ基因表达载体对人口腔鳞癌细胞株HSC-4和人口腔鳞癌细胞株NA的转化我们成功地将IFN-γ基因表达载体（pAdex 1CAmINF-γ）转化到培养的HSC-4和NA细胞中。从pAdex 1CamINF-r转化的HSC-4细胞或NA细胞培养基中检测到INF-r. pAdex 1CAmINF-r转化的HSC-4细胞或人口腔鳞状细胞癌（NA）的表征基因转化不改变细胞的增殖。INF-r的产生水平在一段时间后下降。用pAdex 1CAmINF-r转化HSC-4细胞或NA细胞建立移植转移小鼠模型，转化率和移植率均很低。我们没有从移植肿瘤中检测到IFN-γ。