Cellular and molecular interactions coordinating the upper lip development of the chick embryo

协调鸡胚上唇发育的细胞和分子相互作用

• 批准号: 17592143
• 负责人: AWAKAMI Masayoshi
• 金额: $ 2.18万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592143/
• 关键词: Development; Gene; Cell; LIM homeodomain; chick embryo; L3; Lhx8; international; Canada; Chick embryo; LIM homeodomain; chick embryo

Vertebrate craniofacial morphology originates from cranial neural crest cells. The neural crest cells move into the maxillary and mandibular regions. After the cell migrations, the mesenchymal cells interact with tissues in their local environment and signals from the forebrain and the surface epithelium.The LIM homeodomain (Lhx) genes are necessary for the differentiation of central nervous system neurons. L3/Lhx8, a member of the LIM homeobox domain family of transcription factors is expressed in the craniofacial regions and essential for the formation of secondary palate.We describe the identification and expression pattern of L3 /Lhx8 during embryogenesis of the chick. The protein sequence of L3/Lhx8 chick EST clone was most homologous to mouse L3/Lhx8. Its amino acid identity was more than 90%. RT-PCR analysis during facial development revealed L3/Lhx8 mRNA in chicken maxillary process was detected at stage 19HH and then the expression gradually increased from stage 27 to 31HH. Frozen sections demonstrated restricted expression of L3/Lhx8 in the maxillary mesenchyme and the apposed corners of the frontonasal mass but never in the overlaying epithelium. When removed the maxillary epithelium at stage 18HH, the embryo developed defect of maxillary bone with cleft lip. Our data suggests that an epithelial-mesenchymal interaction induced L3/Lhx8 expression, which may be responsible for development and formation of the upper jaw.

脊椎动物的颅面形态起源于脑神经脊细胞。神经脊细胞移入上颌和下颌区。细胞迁移后，间充质细胞与其局部环境中的组织以及来自前脑和表面上皮细胞的信号相互作用，LIM同源结构域(LIM Homeodomain，LHx)基因是中枢神经系统神经元分化所必需的。L3/Lhx8是LIM同源盒结构域转录因子家族中的一员，在头面部表达，对次级腭部的形成起重要作用。L3/Lhx8鸡EST克隆的蛋白质序列与小鼠L3/Lhx8的同源性最高。其氨基酸同源性在90%以上。面部发育过程中的RT-PCR分析表明，L3/Lhx8基因在鸡上颌突中的表达在19HH期开始表达，从第27期到31HH，L3/Lhx8基因的表达逐渐增强。冰冻切片显示L3/Lhx8在上颌骨间充质和额鼻肿块的对角表达受限，但在覆盖的上皮中不表达。胚胎于18h切除上颌骨上皮后，出现上颌骨缺损性唇裂。我们的数据表明，上皮-间充质相互作用诱导了L3/Lhx8的表达，这可能与上颌的发育和形成有关。

项目成果

L3/Lhx8 is a pivotal factor for cholinergic differentiation of murine embryonic stem cells

• DOI: 10.1038/sj.cdd.4402106
• 发表时间: 2007-06-01
• 期刊: CELL DEATH AND DIFFERENTIATION
• 影响因子: 12.4
• 作者: Manabe, T.;Tatsumi, K.;Wanaka, A.
• 通讯作者: Wanaka, A.

Maximum Anchorage in Lingual Orthodontic Premolar Extraction Treatment of Bidentoalveolar Protrusion.

舌侧正畸前磨牙拔除治疗牙槽突的最大支抗。

• 期刊: OrthoTADs The Clinical Guide and Atlas, edited by J.B. Cope, Under Dog Media, LP, USA
• 作者: Kobayashi T;Ito S;Yasuda K;Kuroda T;Yamamoto K;Sugita N;Tai H;Narita I;Gejyo F;Yoshie H.;Masayoshi Kawakami
• 通讯作者: Masayoshi Kawakami

Expression and regulation of the LIM homeodomain gene L3/Lhx8 suggests a role in upper lip development of the chick embryo

• DOI: 10.1007/s00429-006-0078-0
• 发表时间: 2006-06-01
• 期刊: ANATOMY AND EMBRYOLOGY
• 作者: Inoue, Masahide;Kawakami, Masayoshi;Wanaka, Akio
• 通讯作者: Wanaka, Akio

Expression of hyperpolarization-activated cyclic nucleotide-gated cation channels in rat dorsal root ganglion neurons innervating urinary bladder

• DOI: 10.1016/j.brainres.2006.08.052
• 发表时间: 2006-11
• 期刊: Brain Research
• 影响因子: 2.9
• 作者: H. Matsuyoshi;N. Masuda;M. Chancellor;V. Erickson;Y. Hirao;W. C. Groat;A. Wanaka;N. Yoshimura

Expression and regulation of the LIM homeodomain gene L3/Lhx8 suggests a role in upper lip development of the chick cmbryo

LIM 同源域基因 L3/Lhx8 的表达和调控表明其在鸡胚上唇发育中发挥作用

• 发表时间: 2006
• 期刊: Anatomy and Embryology (in press)
• 作者: Inoue M;Kawakami M;Tasumi K;Manabe T;Makinodan M;Matsuyoshi H;Kirita T;Wanaka A
• 通讯作者: Wanaka A