confocal microscope for 4D imaging of multicellular structure and activity

用于多细胞结构和活性 4D 成像的共焦显微镜

• 批准号: 465594799
• 金额: --
• 依托单位: Ludwig-Maximilians-Universität München (LMU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/465594799?language=en
• 关键词: confocal; microscope; 4D; imaging; multicellular

The basics of the structure formation of multicellular systems is a current question in the physics of active matter. In recent years, in addition to genetic regulation, the role of mechanical forces has also become an independent subject, mechanobiology. The differentiation of stem cells and the associated growth of multicellular systems has progressed so far that artificial "organoids" can be created, which have enormous potential in basic research, but also for pharmaceutical test studies. A fundamental question in the field is how growth can be controlled by artificial nano- and microstructures in the extracellular environment and how the desired cellular functions can be switched on.The newly established junior research group led by Dr. Serwane is studying the growth of artificial neural networks (retina organoids) from individual cells as part of his ERC Starting Grant project ROMB. In the working group of Prof Rädler, cell migration of individual cells and cell clusters in artificial nano- and micro-constructs is investigated within the framework of the SFB 1032 (Nanoagents) "spatiotemporal control of molecular and cellular processes".In this context, a joint confocal microscope is applied for, which allows scales in the range from a few hundred nanometers to several millimeters to be displayed in complex 3D cell systems, as well as to test and manipulate them using laser-based techniques. It is essential that the confocal microscope is able to record cell behavior in physiological and artificial environments over a longer period of time (4D image).The following criteria are of central importance for the projects: 1. Different laser lines in order to be able to image different cellular components. 2. Spatial resolution with which nanostructured environments are recorded (<200nm), 3. Fast fluorescence measurements (Ca imaging) to read out neuronal responses (<25 ms per image, with an optimal signal-to-noise ratio) 4. Low phototoxicity for 4D measurements over 48h. 5. Fast spectral detection to characterize local light-matter interaction. A corresponding device is not available either at the chair or in the vicinity of the physics faculty in the city center. The procurement is to replace a 19-year-old confocal microscope from the original equipment at the Rädler chair.

多细胞系统结构形成的基本原理是当前活性物质物理学中的一个问题。近年来，除了基因调控之外，机械力的作用也成为一门独立的学科——机械生物学。干细胞的分化和相关的多细胞系统的生长已经取得了很大的进展，人工“类器官”可以被创造出来，这在基础研究和药物试验研究中具有巨大的潜力。该领域的一个基本问题是如何在细胞外环境中通过人工纳米和微观结构来控制生长，以及如何开启所需的细胞功能。由Serwane博士领导的新成立的初级研究小组正在研究单个细胞的人工神经网络（视网膜类器官）的生长，这是他的ERC启动资助项目ROMB的一部分。在Rädler教授的工作组中，在SFB 1032（纳米试剂）“分子和细胞过程的时空控制”的框架内，研究了人工纳米和微观结构中单个细胞和细胞簇的细胞迁移。在这种情况下，应用了一种联合共聚焦显微镜，它允许在复杂的3D细胞系统中显示从几百纳米到几毫米的尺度，以及使用基于激光的技术对它们进行测试和操作。重要的是，共聚焦显微镜能够在较长时间内记录生理和人工环境中的细胞行为（4D图像）。以下标准对项目至关重要：1。不同的激光线，以便能够成像不同的细胞成分。2. 2 .记录纳米结构环境的空间分辨率（<200nm）；快速荧光测量（Ca成像）读取神经元响应（每张图像<25 ms，具有最佳信噪比）48h以上4D测量低光毒性。5. 快速光谱检测表征局部光-物质相互作用。无论是在椅子上还是在市中心的物理学院附近，都没有相应的设备。此次采购是为了替换Rädler椅子上一台已有19年历史的共聚焦显微镜。