Combination of ultrahigh‑performance liquid chromatography and high resolution mass spectometry

超高效液相色谱与高分辨率质谱的结合

• 批准号: 467367967
• 金额: --
• 依托单位: Technische Universität Dresden
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/467367967?language=en
• 关键词: Combination; ultrahigh; performance; liquid; chromatography

The equipment applied for is a combination of an ultra-high performance liquid chromatography (UHPLC) instrument for substance separation with a high-resolution mass spectrometer (coupling of a quadrupole system with a time-of-flight mass spectrometer, QTOF-MS, hereinafter referred to as HR-MS) of the latest technical design. The instrument will be used for the structural elucidation of unknown organic trace compounds in environmental samples and other matrices.The research of the applicant institution includes in particular the occurrence, properties and behavior of predominantly anthropogenic chemicals entering the water cycle as well as the tracking of biotic and abiotic transformation processes in technical and natural systems. The availability of a HR-MS is essential for the characterization of degradation pathways and for the identification of products whose environmental relevance also needs to be elucidated. Furthermore, the instrument is needed for the identification of anthropogenic trace pollutants in all stages of the global water cycle, whereby in particular the “Non-Target Screening” is to be used as a methodological approach. Various current and planned research activities require the reliable identification of metabolites and transformation products for a large number of environmental chemicals. The high-resolution LC-MS system is also suitable for the precise quantification of organic trace substances, which is of considerable importance for the applicants too. The planned acquisition will further increase the already existing high-quality and multifaceted research and significantly increase the competitiveness and attractiveness of all participating institutions of the Faculty of Environmental Sciences in particular within the education of young scientists. There is currently no HR-MS available at the entire faculty.

申请的设备是用于物质分离的超高效液相色谱仪与最新技术设计的高分辨率质谱仪（四极杆系统与飞行时间质谱仪的耦合，QTOF-MS，以下简称HR-MS）的组合。该仪器将用于环境样品和其他基质中未知有机痕量化合物的结构解析，申请机构的研究特别包括进入水循环的主要人为化学品的发生、性质和行为，以及跟踪技术和自然系统中的生物和非生物转化过程。HR-MS的可用性对于表征降解途径和识别其环境相关性也需要阐明的产品至关重要。此外，还需要该文书来查明全球水循环所有阶段中的人为痕量污染物，特别是将“非目标筛选”作为一种方法。目前和计划中的各种研究活动需要可靠地鉴定大量环境化学品的代谢物和转化产物。高分辨率LC-MS系统也适用于有机痕量物质的精确定量，这对申请人也相当重要。计划中的收购将进一步增加现有的高质量和多方面的研究，并显着提高环境科学学院所有参与机构的竞争力和吸引力，特别是在年轻科学家的教育方面。目前没有HR-MS可在整个学院。