High resolution tandem mass spectrometer coupled to ultrahigh performance liquid chromatography

高分辨率串联质谱仪与超高效液相色谱联用

• 批准号: 465377122
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/465377122?language=en
• 关键词: resolution; tandem; mass; spectrometer; coupled

We aim to acquire an analytical system consisting of a ultra high performance liquid chromatograph and a high resolution tandem mass spectrometer. The chromatographic system should include a binary high pressure solvent pump, temperature-controlled column compartment, an automatic injection system with coolable sample compartment as well as a diode array detector. For the high resolution mass spectrometry system high mass accuracy and sensitivity for the determination of accurate molecular masses of low abundant and unknown compounds. Furthermore, the system must include the option for tandem mass spectrometry and the parallel acquisition of ion mobility spectrometry data would be beneficial. An important aspect is also a powerful software solution for data processing and interpretation of the resulting data.The principal applications for the new system lie in the untargeted analysis of lipids and other small molecules in complex biological and food samples. One focus is on the assessment of lipidome changes during the preparation and processing of food and the determination of the resulting oxidized lipids. The system will, among other applications, be used to create a systematic database with of lipid oxidation products and to improve the understanding which compounds are formed in food and how the preparation conditions influence the formation. Further projects deal with the characterization of bioactive food constituents and their metabolites, the structural elucidation of sugar degradation products as well as to assess the role of lipid systems in the brain in connection with mental illnesses.

我们的目标是获得一个分析系统组成的超高效液相色谱仪和高分辨率串联质谱仪。色谱系统应包括二元高压溶剂泵、温控柱室、带可冷却样品室的自动进样系统以及二极管阵列检测器。对于高分辨率质谱系统，高质量准确度和灵敏度用于测定低丰度和未知化合物的准确分子量。此外，该系统必须包括串联质谱法的选项，并且并行获取离子迁移谱数据将是有益的。一个重要的方面是一个强大的软件解决方案，用于数据处理和结果数据的解释。新系统的主要应用在于对复杂生物和食品样品中的脂质和其他小分子进行非靶向分析。其中一个重点是评估食品制备和加工过程中脂质体的变化，以及测定由此产生的氧化脂质。除其他应用外，该系统将用于创建脂质氧化产物的系统数据库，并提高对食品中形成的化合物以及制备条件如何影响形成的理解。进一步的项目涉及生物活性食品成分及其代谢物的特性，糖降解产物的结构说明以及评估脂质系统在大脑中与精神疾病有关的作用。