Modeling actin-microtubule crosstalk during centrosome positioning in cell migration and division

中心体的正确定位在有丝分裂、细胞内货物运输和极化迁移以及免疫细胞激活和杀伤过程中至关重要。越来越清楚的是，微管和肌动蛋白细胞骨架在这个核心细胞过程中共同作用，它们的功能动态特性经常密切交织在一起。在这个项目中，我们的目标是了解细胞几何结构、微管、肌动球蛋白网络以及这些成分之间的串扰在间期细胞中中心体定位中的作用。我们计划建立一个计算模型，以研究动态变化的细胞形状和肌动蛋白皮层产生的力对静态和迁移细胞中中心体定位的影响。反之亦然，众所周知，中心体位置与迁移细胞的运动方向密切相关，我们的目标是用我们的计算模型来分析。我们的最终目标是确定肌动蛋白-微管串扰机制，使阿米巴样迁移细胞能够在细胞外基质等复杂的三维环境中导航。

The correct positioning of the centrosome is crucial during mitosis, intracellular cargo transport and polarization in migration and immune cell activation and killing. It is becoming increasingly clear that the microtubule and actin cytoskeleton work together in this core cellular processes and that their functional dynamic properties are often intimately intertwined. In this project, we aim to understand the role of cellular geometry, microtubule, actomyosin network, and crosstalk among these components in positioning the centrosome in the interphase cell. We plan to develop a computational model to study the influence of a dynamically changing cell shape and actin cortex generated forces on the centrosome positioning centrosome in static and migrating cells in two and three dimensions. Vice versa it is known that centrosome position strongly correlates with the direction of movement in migrating cells, which we aim to analyze with our computational model. Our ultimate goal is to identify the actin-microtubule crosstalk mechanisms that enables amoeboid migrating cells to navigate through a complex three-dimensional environment like extracellular matrices.