Modeling actin-microtubule crosstalk during centrosome positioning in cell migration and division

细胞迁移和分裂中中心体定位过程中肌动蛋白-微管串扰的建模

• 批准号: 468346334
• 负责人: Professor Dr. Heiko Rieger
• 金额: --
• 依托单位: Department of Biotechnology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/468346334?language=en
• 关键词: Modeling; actin; microtubule; crosstalk; during

The correct positioning of the centrosome is crucial during mitosis, intracellular cargo transport and polarization in migration and immune cell activation and killing. It is becoming increasingly clear that the microtubule and actin cytoskeleton work together in this core cellular processes and that their functional dynamic properties are often intimately intertwined. In this project, we aim to understand the role of cellular geometry, microtubule, actomyosin network, and crosstalk among these components in positioning the centrosome in the interphase cell. We plan to develop a computational model to study the influence of a dynamically changing cell shape and actin cortex generated forces on the centrosome positioning centrosome in static and migrating cells in two and three dimensions. Vice versa it is known that centrosome position strongly correlates with the direction of movement in migrating cells, which we aim to analyze with our computational model. Our ultimate goal is to identify the actin-microtubule crosstalk mechanisms that enables amoeboid migrating cells to navigate through a complex three-dimensional environment like extracellular matrices.

中心体的正确定位在有丝分裂、细胞内货物运输和迁移中的极化以及免疫细胞活化和杀伤过程中至关重要。越来越清楚的是，微管和肌动蛋白细胞骨架在这一核心细胞过程中一起工作，并且它们的功能动态特性经常密切交织在一起。在这个项目中，我们的目标是了解细胞的几何形状，微管，肌动球蛋白网络，以及这些组件之间的串扰定位在间期细胞中心体的作用。 我们计划开发一个计算模型来研究动态变化的细胞形状和肌动蛋白皮质产生的力对中心体定位中心体在静态和迁移细胞中的二维和三维的影响。反之亦然，众所周知，中心体位置与迁移细胞的运动方向密切相关，我们的目标是用我们的计算模型来分析。我们的最终目标是确定肌动蛋白微管串扰机制，使变形虫迁移细胞通过一个复杂的三维环境，如细胞外基质导航。