Interactions between JA and ABA in fruit and synthesis of JA derivatives shich is effective in field

水果中JA与ABA的相互作用及田间有效的JA衍生物的合成

基本信息

批准号：
14560031
负责人：
KONDO Satoru
金额：
$ 2.18万
依托单位：
Hiroshima Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560031/
关键词：
Physiologically active substances Jasmonic acid Abscisic acid Phaseic acid dihydro phaseic acid xanthoxin

项目摘要

The interactions between JA and ABA were investigated in some fruits.A suitable internal standard for the measurement of natural xanthoxin (XAN) was developed. The relative amounts of ABA in the pulp increased toward apple fruit maturation. On a molar basis, the amount of total XAN (cis,trans-XAN and trans,trans-XAN) in immature fruit was equal to the amount of ABA. However during the fruit ripening process, the total XAN levels markedly decreased in proportion with increases in the levels of ABA. At the climacteric rise and post climacteric stage ABA concentrations were higher than the total amount of XAN. The low levels of XAN in a ripening apple may be caused by to its rapid turnover. The total XAN concentration in the seeds was high in immature seeds but decreased toward seed maturation. The opposite tendency was observed in the case of ABA concentration. Thus, decreases of total XAN correlated with increases in the ABA levels ; this tendency was observed in both the pulp and seeds … More , and was consistent with the role of XAN as an ABA precursor. In the pulp, PA concentrations changed in a manner similar to those of ABA. The maximum epi-DPA concentration was 67% of the maximum ABA concentration, but the maximum DPA concentration was only 11% of the maximum ABA concentration. Therefore, epi-DPA was thought to be a major metabolite of PA in the pulp. The derivatives of Jasmonic acid (JA) which are effective in the field were developed. The applications of some kinds of JA derivatives increased anthocyanin accumulation of apples in the field.JA and methyl jasmonate (MeJA) were quantified in the skin, pulp, and seeds of mangoes (Mangifera indica L.). JA concentrations in the skin were higher than those in the pulp. 1-aminocyclopropane-1 -carboxylic acid (ACC) concentrations in the skin and pulp increased toward harvest. Differing with JA,ACC concentrations in the pulp were high compared with the skin. This fact suggests that although JA and ACC are associated with the ripening of mangoes, they may play different roles. JA concentrations in the seeds, of both cultivars decreased toward harvest. It is possible that the result indicates a lack of dormancy in mango seeds. Changes of jasmonates during storage were also examined. JA levels in the skin and pulp increased in stored fruit. In addition, the degree of JA increase was large in fruit which lost much weight. It is assumed that JA associated with fruit senescence was caused by moisture loss. Less

在某些水果中研究了JA和ABA之间的相互作用。开发了天然Xanthoxin（XAN）的合适内标。果肉中ABA的相对量增加了苹果果实的成熟。在摩尔的基础上，未成熟果实中的总XAN（顺式，trans-xan和trans，trans-xan）的量等于ABA的量。但是，在果实成熟过程中，随着ABA水平的增加，XAN的总水平显着降低。在高潮升高和高潮阶段，ABA浓度高于XAN的总量。成熟苹果中的XAN水平低可能是由于其快速营业额引起的。种子中的总Xan浓度很高，但未成熟的种子却降低了种子成熟。在ABA浓度的情况下，观察到了相反的趋势。这是XAN的减少与ABA水平的增加相关。在果肉和种子中都观察到了这种趋势……更多，并且与Xan作为ABA前体的作用一致。在纸浆中，PA浓度以类似于ABA的方式变化。最大EPI-DPA浓度为最大ABA浓度的67％，但最大DPA浓度仅为最大ABA浓度的11％。因此，EPI-DPA被认为是纸浆中PA的主要代谢物。在现场开发了有效的茉莉酸（JA）的衍生物。某种类型的JA衍生物的应用增加了苹果在田野中的花青素积累。JA和jasmonate（MEJA）在芒果的皮肤，果肉和种子中定量（Mangifera Indiona L.）。皮肤中的JA浓度高于纸浆中的JA浓度。 1-氨基丙烷-1-皮肤和果肉中的羧酸（ACC）浓度增加了收获。与JA不同，与皮肤相比，纸浆中的ACC浓度很高。这一事实表明，尽管JA和ACC与芒果成熟有关，但它们可能扮演不同的角色。种子中的JA浓度，两个品种的浓度都降低了收获。结果可能表明芒果种子缺乏休眠。还检查了在存储过程中的牙线变化。储存的水果中的皮肤和果肉中的JA水平增加。此外，JA的增加程度很大，水果减轻了很多。假定与果实感应相关的JA是由水分流失引起的。较少的