Interactions between JA and ABA in fruit and synthesis of JA derivatives shich is effective in field

在一些果实中研究了JA和ABA的相互作用。建立了测定天然黄原素含量的内标。果肉中ABA的相对含量随果实成熟而增加。在摩尔基础上，未成熟果实中总XAN（顺式、反式XAN和反式、反式XAN）的量与ABA的量相等。在果实成熟过程中，总XAN水平随ABA水平的增加而显著降低。在更年期上升和绝经后ABA浓度高于XAN的总量。成熟苹果中XAN含量低可能是由于其快速周转所致。种子中总XAN浓度在未成熟时较高，但随着种子成熟而降低。在ABA浓度的情况下，观察到相反的趋势。因此，总XAN的减少与ABA水平的增加相关；在果肉和种子中都观察到这种趋势，这与XAN作为ABA前体的作用是一致的。在果肉中，PA浓度的变化方式与ABA相似。epi-DPA最大浓度为ABA最大浓度的67%，而DPA最大浓度仅为ABA最大浓度的11%。因此，epi-DPA被认为是牙髓中PA的主要代谢物。开发了茉莉酸（Jasmonic acid， JA）在田间有效的衍生物。部分JA衍生物的施用增加了田间苹果花青素的积累。测定了芒果果皮、果肉和种子中JA和茉莉酸甲酯（MeJA）的含量。果皮JA浓度高于牙髓JA浓度。1-氨基环丙烷-1 -羧酸（ACC）在果皮和果肉中的浓度随着收获而增加。与JA不同，果肉中的ACC浓度高于皮肤。这一事实表明，尽管JA和ACC与芒果的成熟有关，但它们可能起着不同的作用。两个品种籽粒中JA浓度在收获期均呈下降趋势。这一结果可能表明芒果种子缺乏休眠。同时考察了茉莉酸盐在贮藏过程中的变化。果皮和果肉中的JA含量在贮藏后增加。此外，在失重较多的果实中，JA的增加程度较大。认为与果实衰老相关的JA是由水分流失引起的。少

The interactions between JA and ABA were investigated in some fruits.A suitable internal standard for the measurement of natural xanthoxin (XAN) was developed. The relative amounts of ABA in the pulp increased toward apple fruit maturation. On a molar basis, the amount of total XAN (cis,trans-XAN and trans,trans-XAN) in immature fruit was equal to the amount of ABA. However during the fruit ripening process, the total XAN levels markedly decreased in proportion with increases in the levels of ABA. At the climacteric rise and post climacteric stage ABA concentrations were higher than the total amount of XAN. The low levels of XAN in a ripening apple may be caused by to its rapid turnover. The total XAN concentration in the seeds was high in immature seeds but decreased toward seed maturation. The opposite tendency was observed in the case of ABA concentration. Thus, decreases of total XAN correlated with increases in the ABA levels ; this tendency was observed in both the pulp and seeds … More , and was consistent with the role of XAN as an ABA precursor. In the pulp, PA concentrations changed in a manner similar to those of ABA. The maximum epi-DPA concentration was 67% of the maximum ABA concentration, but the maximum DPA concentration was only 11% of the maximum ABA concentration. Therefore, epi-DPA was thought to be a major metabolite of PA in the pulp. The derivatives of Jasmonic acid (JA) which are effective in the field were developed. The applications of some kinds of JA derivatives increased anthocyanin accumulation of apples in the field.JA and methyl jasmonate (MeJA) were quantified in the skin, pulp, and seeds of mangoes (Mangifera indica L.). JA concentrations in the skin were higher than those in the pulp. 1-aminocyclopropane-1 -carboxylic acid (ACC) concentrations in the skin and pulp increased toward harvest. Differing with JA,ACC concentrations in the pulp were high compared with the skin. This fact suggests that although JA and ACC are associated with the ripening of mangoes, they may play different roles. JA concentrations in the seeds, of both cultivars decreased toward harvest. It is possible that the result indicates a lack of dormancy in mango seeds. Changes of jasmonates during storage were also examined. JA levels in the skin and pulp increased in stored fruit. In addition, the degree of JA increase was large in fruit which lost much weight. It is assumed that JA associated with fruit senescence was caused by moisture loss. Less