Drought tolerance in apple seedlings through abscisic acid (ABA) regulation

通过脱落酸（ABA）调节苹果幼苗的耐旱性

• 批准号: 19380025
• 负责人: KONDO Satoru
• 金额: $ 10.4万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380025/
• 关键词: 果樹; ABA8'立水酸化酵素; アブシシン酸; リンゴ; 気孔開度; 水分ポテンシャル; ABA8'水酸化酵素

In this study, we examined the effect of an inhibitor of ABA 8'-hydroxylase (AHI) on drought tolerance in apple seedlings (Malus domestica Borkh.). ABA metabolism, stomatal aperture, water potential, and radical scavenging activity in leaves were all investigated under drought conditions. The result of our observation had shown that the AHI application to seedlings greatly decreased the stomatal aperture in the leaves as in contrast to untreated control samples.However, watering of AHI treated plants re-increased it to the original level. In addition, water potential in AHI-treated leaves remained higher than the one measured in untreated-leaves during the exposure to drought. Drought conditions increased ABA concentrations in both, AHI treated-plants and untreated-plant specimens. Notably, ABA concentrations in AHI-treated leaves were higher than those in untreated-leaves under drought conditions. Although the expression of 9-cis-epoxycarotenoid dioxigenase (MdNCED) genes increased with days of drought stress treatment, in AHI-treated leaves the expression of those genes was significantly lower than in untreated control samples.These results suggest that the prompt stomatal closure through the regulation of ABA 8'-hydroxylase may be required for the plants to survive under relatively long drought conditions.

在这项研究中，我们检查了ABA 8'-羟化酶（AHI）抑制剂对苹果幼苗干旱耐受性的影响（Malus housea borkh。）。在干旱条件下研究了ABA代谢，气孔孔，水潜能和叶片的根治清除活性。我们观察结果的结果表明，与未经处理的对照样品相比，幼苗的应用大大降低了叶片的气孔孔。此外，经AHI处理的叶子中的水潜力仍然高于在暴露于干旱期间未经处理的叶片中测量的水。干旱条件增加了AHI处理的植物和未处理植物标本的ABA浓度。值得注意的是，在干旱条件下，AHI处理的叶片中的ABA浓度高于未经处理的叶片的ABA浓度。尽管随着干旱压力治疗的天数的表达，9-CIS-环氧二氧化酶（MDNNCCED）基因的表达增加，但在经过AHI处理的叶片中，这些基因的表达明显低于未经处理的对照样品中这些基因的表达。这些结果表明，这些结果表明，通过ABA 8'-Hydroxylase的调节，这些基因的迅速闭合可能会导致该疾病的相互关系，以使其相互关联。

项目成果

アブシシン酸(ABA)代謝制御によるリンゴ実生の乾燥耐性向上

通过调节脱落酸（ABA）代谢提高苹果幼苗的耐旱性

• 发表时间: 2010
• 作者: Nakamura;Y.;T.Gotoh;S.Imanishi;K.Mita;T.J.Kurtti;H.Noda;Taiki Narabayashi;近藤悟・二宮麻希・大川克哉・小原均・上野琴巳・轟康司・水谷正治・菅谷純子・平井伸博;Siriruk Sarawaneeyaruk;近藤悟
• 通讯作者: 近藤悟

アブシシン酸 (ABA) 代謝制御によるリンゴ実生の乾燥耐性向上

通过控制脱落酸（ABA）代谢提高苹果幼苗的耐旱性

• 发表时间: 2010
• 作者: Nakamura;Y.;T.Gotoh;S.Imanishi;K.Mita;T.J.Kurtti;H.Noda;Taiki Narabayashi;近藤悟・二宮麻希・大川克哉・小原均・上野琴巳・轟康司・水谷正治・菅谷純子・平井伸博
• 通讯作者: 近藤悟・二宮麻希・大川克哉・小原均・上野琴巳・轟康司・水谷正治・菅谷純子・平井伸博