Changes of jasmonates in climacteric and nonclimacteric fruit

跃变型和非跃变型果实中茉莉酸的变化

• 批准号: 11660036
• 负责人: KONDO Satoru
• 金额: $ 1.92万
• 依托单位: Hiroshima Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660036/
• 关键词: Jasmonic acid; Methyl jasmonate; Fruit development; Fruit ripening; Anthocyanin; Apple; Peach; Phytohormone; 細胞分裂; 果皮着色; PGR

Trans-jasmonic acid (JA), cis-JA, and trans-methyl jasmonate (MeJA) were quantified in pulp and seeds of 'Tsugaru' apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] and 'Satohnishiki' sweet cherry (Prunus avium L.). JA and MeJA concentrations were high in the early growth stages of apple pulp development, decreased with days after full bloom (DAFB), and then increased again during maturation. Concentrations of JA and MeJA in the pulp of sweet cherry were high during early growth stages, then decreased towards harvest. JA concentration in apple seeds was also high in the early growth stages, then decreased, and finally peaked at harvest. MeJA concentration in apple seeds increased towards harvest. In the seeds of sweet cherry, JA and MeJA concentrations generally increased until harvest. In both types of fruit, concentrations of JA and MeJA in the seeds were higher than those of pulp. These results demonstrate that relatively high amounts of JA and MeJA are associated w … More ith young developing fruit. These substances may have a role in regulation of fruit growth at early growth stages, though this has not been demonstrated. The role of JA in callus formation was investigated on discs taken from the pulp of apple, sampled on 15, 25, and 35 DAFB, and cultured on B5 medium, containing combinations of naphthalene acetic acid (NAA), benzyladenin (BA), and JA.On the 15 DAFB sample, 0.45 μmol, 1.0 μmol, and 4.5 μmol JA in the presence of 10.7 μmol NAA induced greater callus formation than did the sample without JA.However, this effect of JA at 15 DAFB was not observed except when it was combined with only NAA ; 4.5 μmol JA inhibited the callus formation. The presence of JA inhibited callus formation on discs taken 25 and 35 DAFB.Endogenous JA concentration of apple pulp at 15 DAFB was lower than that at 25 and 35 DAFB.These results indicate that the low endogenous JA concentration early in fruit development promotes callus formation ; whereas, increasing JA concentration inhibits it. The discs with skins from the apple pulp samples from preclimacteric, climacteric and postclimacteric stages were excised, cultured for 7 days under light on B5 media containing MeJA, and the combination of MeJA and AVG, and the effect of jasmonates on anthocyanin accumulation was investigated. In each sampling stage, MeJA alone and MeJA combined with AVG both stimulated greater anthocyanin accumulation compared to untreated control, though the combination of MeJA and AVG resulted in a lower concentration of anthocyanin than those of only MeJA at the preclimacteric and postclimacteric stages. Therefore, in most cases, jasmonates may be related to anthocyanin formation in apples independent of ethylene. Less

对‘Tsugaru’苹果果肉和种子中的反式茉莉酸（JA）、顺式茉莉酸（顺式茉莉酸）和反式茉莉酸甲酯（MeJA）进行了定量分析。轧机。家蝇变种（伯克氏）Mansf。和‘Satohnishiki’甜樱桃（Prunus avium L.）。JA和MeJA浓度在苹果果肉发育的早期较高，随着开花时间的增加而降低，成熟过程中再次升高。甜樱桃果实中JA和MeJA含量在生长初期较高，在收获期逐渐降低。苹果种子中JA浓度在生长初期较高，随后逐渐降低，并在收获时达到峰值。苹果种子中MeJA浓度随着收获期的增加而增加。在甜樱桃种子中，JA和MeJA浓度在收获前普遍升高。在两种水果中，种子中JA和MeJA的浓度均高于果肉。这些结果表明，相对高剂量的JA和MeJA与幼龄发育果实的关系更大。这些物质可能在水果生长的早期阶段有调节作用，尽管这还没有得到证实。研究了JA在苹果果肉愈伤组织形成中的作用，分别在15、25和35 DAFB上取样，并在含有萘乙酸（NAA）、苯ladenin （BA）和JA的B5培养基上培养。在15 DAFB样品中，在添加10.7 μmol NAA的情况下，0.45 μmol、1.0 μmol和4.5 μmol JA诱导的愈伤组织形成量大于不添加JA的愈伤组织形成量。然而，在15 DAFB时，除了仅与NAA联合使用外，没有观察到JA的这种作用；4.5 μmol JA抑制愈伤组织的形成。JA的存在抑制了25和35 DAFB椎间盘的愈伤组织形成。15 DAFB处理苹果果肉的内源JA浓度低于25和35 DAFB处理。结果表明，果实发育早期较低的内源JA浓度促进了愈伤组织的形成；而增加JA浓度则对其有抑制作用。将绝经前、绝经期和绝经后苹果果肉样品的带皮盘切除，在含MeJA和MeJA与AVG复合的B5培养基上光照培养7 d，研究茉莉酸盐对花青素积累的影响。在每个采样阶段，MeJA单独和MeJA联合AVG都比未处理的对照组刺激了更多的花青素积累，尽管MeJA和AVG联合在绝经前和绝经后阶段的花青素浓度低于单独使用MeJA。因此，在大多数情况下，茉莉酸可能与苹果中花青素的形成有关，而不依赖于乙烯。少

项目成果

Kondo,S: "Changes of endogenous jasmonic acid and methyl jasmonate in apples and weet cherries during fruit development"Journal of the American Society for Horticultural Science. 125(3). 282-287 (2000)

Kondo，S：“苹果和湿樱桃在果实发育过程中内源茉莉酸和茉莉酸甲酯的变化”美国园艺学会杂志。

Kondo,S: "Stimulative effect of jasmonic acid on callus formation in apple fruit"Journal of the Japanese Society for Horticultural Science. 70(2)(予定). (2001)

Kondo,S：“茉莉酸对苹果果实愈伤组织形成的促进作用”日本园艺学会杂志 70(2)（计划）。

Satoru Kondo 他: "Interactions between jasmonates and abscisic acid in apple fruit, and stimulative effect of jasmonates on anthocyanin accumulation"Journal of the Japanese Society for Horticultural Science. 70(5)(印刷中). (2001)

Satoru Kondo 等人：“苹果果实中茉莉酸和脱落酸的相互作用，以及茉莉酸对花青素积累的促进作用”，日本园艺学会杂志 70(5)（出版中）。

Satoru Kondo, Naoko Tsukada and Yoshiyuki Niimi.: "Stimulative effect of jasmonic acid on callus formation in apple fruit."Journal of the Japanese Society for Horticultural Science.. 70 (2). 229-231 (2001)

Satoru Kondo、Naoko Tsukada 和 Yoshiyuki Niimi.：“茉莉酸对苹果果实愈伤组织形成的刺激作用。”日本园艺学会杂志。70 (2)。

Satoru Kondo 他: "Changes of endogenous jasmonic acid and methyl jasmonate in apples and sweet cherries during fruit development"Journal of the American Society for Horticultural Science. 125(3). 282-287 (2000)

Satoru Kondo 等人：“苹果和甜樱桃中内源茉莉酸和茉莉酸甲酯在果实发育过程中的变化”美国园艺科学杂志 125(3) 282-287 (2000)。