Signal transduction mechanisms on the regulation of the acrosome reaction and the motility of fowl spermatozoa.

为了探讨哪些蛋白磷酸酶参与了鸡精子运动和顶体反应（AR）的调控，本研究考察了40℃条件下某些蛋白磷酸酶抑制剂对精子运动和顶体反应的影响。不论卵泡内周层（IPVL）是否存在，10 ~ 100 nM的他莫霉素均能恢复精子在40℃下的活力，而1000 nM的他莫霉素几乎不能恢复精子在40℃下的活力。当IPVL存在时，通过加入他霉素刺激AR。此外，无论是否存在IPVL，在加入他霉素（100 nM）后再加入2 mM Ca^<2+>都能有效地恢复40℃下的运动，并刺激AR。另一方面，钙素A的加入恢复了精子的运动能力，并刺激了AR。在钙素A （100 nM）加入后，在40℃下添加2 mM Ca^<2+>对精子运动能力的恢复是有效的，并刺激了更多的AR。此外，无论是否存在IPVL，与添加花青素a相比，添加冈田酸都不能完全恢复精子在40℃下的活力。然而，当存在IPVL时，添加冈田酸刺激了AR。无论是否存在IPVL，添加染料木黄碱都不能恢复精子在40℃下的活力。染料木黄碱在0 ~ 10 μM范围内对AR有刺激作用，而100 μM的染料木黄碱几乎没有刺激作用。此外，无论是否存在IPVL，在0-10 μM范围内，加入染料木素后再加入2mM Ca^<2+>，都能有效地恢复40℃下的运动。以上结果提示，鸡精子的运动可能受PP1的调控，但精子的AR可能受PP1和PP2A的调控，精子的运动和AR可能受PTK或PKC的调控。但是，PKA似乎与精子的运动和AR无关。少

In order to investigate what sort of protein phosphatases are involved in the regulation of fowl sperm motility and acrosome reaction(AR), the influence of certain protein phosphatase inhibitors on the motility and AR of spermatozoa at 40℃ was examined. Regardless of whether the inner perivitelline layers(IPVL) present or not, the motility of spermatozoa at 40℃ was restored by the addition of 10-100 nM tautomycin, but did not almost restored by the addition of 1000 nM tautomtcin. When IPVL was present, AR was stimulated by the addition of tautomycin. Furthermore, regardless of whether IPVL present or not, an addition of 2 mM Ca^<2+> after the addition of tautomycin (100 nM) was effective for the recover of motility at 40℃, and the AR was also stimulated. On the other hand, an addition of calyculin A recovered the motility of spermatozoa, and stimulated the AR. An addition of 2 mM Ca^<2+> after the addition of calyculin A (100 nM) was effective for the recover of motility at 40℃, and th … More e AR was also stimulated. In addition, regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not almost restored by the addition of okadaic acid, compared with those of the addition of calyculin A. However, when IPVL was present, AR was stimulated by the addition of okadaic acid. Regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not restored by the addition of genisteine. The AR was stimulated by the addition of genisteine within the range of 0-10 μM, but did not almost stimulated by the addition of 100 μM genisteine. Furthermore, regardless of whether IPVL present or not, an addition of 2mM Ca^<2+> after the addition of genisteine within the range of 0-10 μM was effective for the recover of motility at 40℃.From the results described above, it is suggested that the motility of fowl spermatozoa may be regulated by PP1, but the AR of spermatozoa may be regulated by PP1 and PP2A, In addition, the motility and AR of spermatozoa may be regulated by PTK or PKC. But, it appears that PKA is not involved with the motility and AR of spermatozoa. Less