Signal transduction mechanisms on the regulation of the acrosome reaction and the motility of fowl spermatozoa.

为了探讨哪些蛋白磷酸酶参与了鸡精子活力和顶体反应(AR)的调节，在40℃时检测了某些蛋白磷酸酶抑制剂对精子活力和AR的影响。无论是否存在卵黄周内层，加入10-100nM的交托霉素均可恢复精子在40℃时的活力，而加入1000nM的交托霉素则不能基本恢复精子的活力。当存在IPVL时，AR可通过加入交托霉素来刺激。此外，无论是否存在IPVL，在加入交托霉素(100 NM)后再加入2 mM的Ca~(2+)和GT~(2+)均能有效地恢复40℃时的运动，并刺激AR。另一方面，加入花盏花素A可恢复精子的活力，并刺激AR。在加入花盏花素A(100 NM)的基础上再加入2 mM的Ca~(2+)，可有效地恢复40℃和…时的运动功能。更多的eAR也受到了刺激。此外，无论是否存在IPVL，加入冈田酸并不能几乎恢复精子在40℃时的活力，但当存在IPVL时，AR被加入冈田酸所刺激。无论是否存在IPVL，添加金雀异黄碱均不能恢复精子在40℃时的活力。在0~10μM范围内，染料木碱对AR有刺激作用，而100μM的染料木碱对AR几乎无刺激作用。此外，无论是否存在IPVL，在0-10μM的范围内加入2 mM的Ca~(2+)和GT~(2+)均能有效地恢复40℃时的活力。以上结果表明，鸡精子的活力可能受PP1调节，但精子的AR可能受PP1和PP2A的调节，此外，精子的活力和AR可能受PTK或PKC的调节。但是，PKA似乎不参与精子的运动和AR。较少

In order to investigate what sort of protein phosphatases are involved in the regulation of fowl sperm motility and acrosome reaction(AR), the influence of certain protein phosphatase inhibitors on the motility and AR of spermatozoa at 40℃ was examined. Regardless of whether the inner perivitelline layers(IPVL) present or not, the motility of spermatozoa at 40℃ was restored by the addition of 10-100 nM tautomycin, but did not almost restored by the addition of 1000 nM tautomtcin. When IPVL was present, AR was stimulated by the addition of tautomycin. Furthermore, regardless of whether IPVL present or not, an addition of 2 mM Ca^<2+> after the addition of tautomycin (100 nM) was effective for the recover of motility at 40℃, and the AR was also stimulated. On the other hand, an addition of calyculin A recovered the motility of spermatozoa, and stimulated the AR. An addition of 2 mM Ca^<2+> after the addition of calyculin A (100 nM) was effective for the recover of motility at 40℃, and th … More e AR was also stimulated. In addition, regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not almost restored by the addition of okadaic acid, compared with those of the addition of calyculin A. However, when IPVL was present, AR was stimulated by the addition of okadaic acid. Regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not restored by the addition of genisteine. The AR was stimulated by the addition of genisteine within the range of 0-10 μM, but did not almost stimulated by the addition of 100 μM genisteine. Furthermore, regardless of whether IPVL present or not, an addition of 2mM Ca^<2+> after the addition of genisteine within the range of 0-10 μM was effective for the recover of motility at 40℃.From the results described above, it is suggested that the motility of fowl spermatozoa may be regulated by PP1, but the AR of spermatozoa may be regulated by PP1 and PP2A, In addition, the motility and AR of spermatozoa may be regulated by PTK or PKC. But, it appears that PKA is not involved with the motility and AR of spermatozoa. Less