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Signal transduction mechanisms on the regulation of the acrosome reaction and the motility of fowl spermatozoa.

顶体反应和家禽精子活力调节的信号转导机制。

基本信息

批准号：
14560236
负责人：
ASHIZAWA Koji
金额：
$ 2.56万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560236/
关键词：
spermatozoa protein phosphatase protein kinase regulation of motility phosphorylation dephosphorylation signal transduction acrosome reaction 鞭毛運動 PP1 カルパイン

项目摘要

In order to investigate what sort of protein phosphatases are involved in the regulation of fowl sperm motility and acrosome reaction(AR), the influence of certain protein phosphatase inhibitors on the motility and AR of spermatozoa at 40℃ was examined. Regardless of whether the inner perivitelline layers(IPVL) present or not, the motility of spermatozoa at 40℃ was restored by the addition of 10-100 nM tautomycin, but did not almost restored by the addition of 1000 nM tautomtcin. When IPVL was present, AR was stimulated by the addition of tautomycin. Furthermore, regardless of whether IPVL present or not, an addition of 2 mM Ca^<2+> after the addition of tautomycin (100 nM) was effective for the recover of motility at 40℃, and the AR was also stimulated. On the other hand, an addition of calyculin A recovered the motility of spermatozoa, and stimulated the AR. An addition of 2 mM Ca^<2+> after the addition of calyculin A (100 nM) was effective for the recover of motility at 40℃, and th … More e AR was also stimulated. In addition, regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not almost restored by the addition of okadaic acid, compared with those of the addition of calyculin A. However, when IPVL was present, AR was stimulated by the addition of okadaic acid. Regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not restored by the addition of genisteine. The AR was stimulated by the addition of genisteine within the range of 0-10 μM, but did not almost stimulated by the addition of 100 μM genisteine. Furthermore, regardless of whether IPVL present or not, an addition of 2mM Ca^<2+> after the addition of genisteine within the range of 0-10 μM was effective for the recover of motility at 40℃.From the results described above, it is suggested that the motility of fowl spermatozoa may be regulated by PP1, but the AR of spermatozoa may be regulated by PP1 and PP2A, In addition, the motility and AR of spermatozoa may be regulated by PTK or PKC. But, it appears that PKA is not involved with the motility and AR of spermatozoa. Less

为了研究哪些蛋白磷酸酶参与了鸡精子活力和顶体反应的调节，研究了几种蛋白磷酸酶抑制剂在40℃下对鸡精子活力和顶体反应的影响。无论内卵周层（IPVL）是否存在，在40℃时，加入10-100 nM互变霉素可恢复精子的活力，但加入1000 nM互变霉素几乎不能恢复精子的活力。当IPVL存在时，AR通过加入互变霉素刺激。此外，无论是否存在IPVL，在加入互变霉素（100 nM）后再加入2 mM Ca^<2+>，在40℃下都能有效恢复运动性，并且AR也被刺激。另一方面，添加calyculin A可恢复精子的活动力，并刺激AR。在加入calyculin A（100 nM）后再加入2 mM Ca^<2+>对40℃下运动性的恢复是有效的， ...更多信息 e AR也被刺激。此外，与添加calyculin A相比，添加冈田酸后，无论IPVL是否存在，精子在40℃下的活动力几乎没有恢复。然而，当IPVL存在时，通过添加冈田酸刺激AR。无论是否存在IPVL，在40℃下加入染料木素均不能恢复精子的活力。在0-10 μM范围内，染料木素对AR有刺激作用，而100 μM染料木素几乎不刺激AR。此外，无论是否存在IPVL，在加入0-10 μM染料木素后再加入2 mM Ca^<2+>，在40℃下都能有效地恢复精子的活力。上述结果表明，鸡精子的活力可能受PP 1的调节，而精子的AR可能受PP 1和PP 2A的调节。精子的运动和AR可能受PTK或PKC的调节。但PKA与精子的活力和AR无关。少