Analysis and application of molecular dynamics in induction of implantation

分子动力学在植入诱导中的分析及应用

• 批准号: 14560249
• 负责人: IWATA Hiroyuki
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560249/
• 关键词: induction of placentation; microarray; food animal production; gene therapy; Lentivirus vector; siRNA methods; tissue specifc knockout

The molecule, which is available for embryonic implantation in mice, has not been discovered without the leukemia inhibitory factor (LIF). So, the molecular dynamics in the implantation was analysed by microarray technology using the LIF KO and the delayed implantation mice. The microarray analysis in this study used MG_U74Av2 array (Affymetrix Co.), which detected the 12,489 genes. We detected the repression of gene expression in 50 genes 4 days after pregnancy in the LIF KO and delayed implantation mice. Next, mice with delayed implantation were produced, and a role of LIF at the implantation period was clarified to be essential for the event. About 80% of the selected 50 genes could be controlled by only LIF. As for LIF treatment, the effects of LIF were arisen in various times from several minutes to 6 hours after increase of LIF in the blood. Furthermore, expression and functional analyses of CD9 revealed that CD9 was increased in gene and protein levels at the implantation period and the inhibition of implantation was observe in CD9 KO mice transplanted with fertilized eggs. As fox gene transfer technology using Lentivirus vector, AP and ZAP genes were introduced in mice and the expressions of these genes were confirmed

这种可用于小鼠胚胎植入的分子，在没有白血病抑制因子（LIF）的情况下还没有被发现。因此，通过微阵列技术使用LIF KO和延迟植入小鼠分析植入的分子动力学。本研究中的微阵列分析使用MG_U74Av2阵列（Affytechnic Co.），它检测了12,489个基因。我们检测了LIF KO和延迟着床小鼠妊娠4天后50个基因表达的抑制。接下来，产生具有延迟植入的小鼠，并且阐明LIF在植入期的作用对于该事件是必不可少的。在所选的50个基因中，约有80%的基因仅受LIF控制。对于LIF治疗，LIF的作用在血液中LIF增加后的几分钟至6小时的不同时间出现。此外，CD 9的表达和功能分析显示，在植入期，CD 9在基因和蛋白水平上增加，并且在移植受精卵的CD 9 KO小鼠中观察到植入抑制。作为使用慢病毒载体的狐狸基因转移技术，将AP和ZAP基因导入小鼠，并证实了这些基因的表达

项目成果

Tanabe S et al.: "Expression of mRNA of chemokine receptor CXCR4 in feline mammary"Vet Rec. 151(24). 729-733 (2002)

Tanabe S 等人：“猫科动物乳腺中趋化因子受体 CXCR4 的 mRNA 表达”Vet Rec。

Tanabe S, Nakadai T, Furuoka H, Oomachi T, Kobayashi Y, Omata Y, Koyama T, Hondo E, Uzuka Y, Sarashina T, Ducusin R J, Shida T, Dorf ME.: "Expression of mRNA of chemokine receptor CXCR4 in feline mammary adenocarcinoma"Vet.Rec.. 151(24). 729-733 (2002)

Tanabe S、Nakadai T、Furuoka H、Oomachi T、Kobayashi Y、Omata Y、Koyama T、Hondo E、Uzuka Y、Sarashina T、Ducusin R J、Shida T、Dorf ME.：“猫科动物趋化因子受体 CXCR4 mRNA 的表达

Kakuni M, Makita T, Wijayanto H, Hondo E, Kiso Y: "Histology of vermiform appendix like organ in slow loris"Exp.Anim.. 52(1). 71-75 (2002)

Kakuni M、Makita T、Wijayanto H、Hondo E、Kiso Y：“懒猴蠕形阑尾样器官的组织学”Exp.Anim.. 52(1)。

Nakamura O et al.: "IGF-1 overexpression causes fetal loss during placentation in mice"J Reprod Dev. in press. (2004)

Nakamura O 等人：“IGF-1 过度表达会导致小鼠胎盘期间胎儿流产”J Reprod Dev。

Shimokawa T, Yamagiwa D, Hondo E, Nishiwaki S, Kiso Y, Makita T: "Histological observation of the proper gastric gland in Minke whale, Balaenoptera acutorostrata"J.Vet.Med.Sci.. 65(3). 423-426 (2003)

Shimokawa T、Yamagiwa D、Hondo E、Nishiwaki S、Kiso Y、Makita T：“小须鲸、Balaenoptera acutorostrata 胃腺的组织学观察”J.Vet.Med.Sci. 65(3)。