Analysis and application of molecular dynamics in induction of implantation

这种可用于小鼠胚胎植入的分子，在没有白血病抑制因子(LIF)的情况下还没有被发现。因此，利用基因芯片技术，以LIF KO和延迟着床小鼠为材料，对植入过程中的分子动力学进行了分析。本研究中的微阵列分析使用了MG_U74Av2阵列(Affymetrix Co.)，检测到了12,489个基因。我们检测了LIF、KO和延迟着床小鼠妊娠4天后50个基因的表达抑制。接下来，产生延迟着床的小鼠，LIF在着床期的作用被阐明为该事件的关键。在所选择的50个基因中，约有80%可由LIF单独控制。对于LIF治疗，LIF的作用在血液中LIF升高后的几分钟到6小时内不同时间出现。此外，CD9的表达和功能分析表明，CD9在着床期基因和蛋白水平均升高，并观察到受精卵移植对CD9 KO小鼠着床的抑制作用。以慢病毒为载体的狐狸基因转移技术，将AP和ZAP基因导入小鼠体内，并证实了这些基因的表达

The molecule, which is available for embryonic implantation in mice, has not been discovered without the leukemia inhibitory factor (LIF). So, the molecular dynamics in the implantation was analysed by microarray technology using the LIF KO and the delayed implantation mice. The microarray analysis in this study used MG_U74Av2 array (Affymetrix Co.), which detected the 12,489 genes. We detected the repression of gene expression in 50 genes 4 days after pregnancy in the LIF KO and delayed implantation mice. Next, mice with delayed implantation were produced, and a role of LIF at the implantation period was clarified to be essential for the event. About 80% of the selected 50 genes could be controlled by only LIF. As for LIF treatment, the effects of LIF were arisen in various times from several minutes to 6 hours after increase of LIF in the blood. Furthermore, expression and functional analyses of CD9 revealed that CD9 was increased in gene and protein levels at the implantation period and the inhibition of implantation was observe in CD9 KO mice transplanted with fertilized eggs. As fox gene transfer technology using Lentivirus vector, AP and ZAP genes were introduced in mice and the expressions of these genes were confirmed