Analysis and application of molecular dynamics in induction of implantation

该分子可用于小鼠胚胎植入，但尚未发现不含白血病抑制因子（LIF）的分子。因此，采用微阵列技术，利用LIF - KO和延迟着床小鼠对着床过程中的分子动力学进行了分析。微阵列分析采用MG_U74Av2阵列（Affymetrix公司），共检测到12489个基因。我们在LIF - KO和延迟着床小鼠妊娠4天后检测了50个基因的表达抑制。接下来，产生了延迟着床的小鼠，并阐明了LIF在着床期的作用对该事件至关重要。所选的50个基因中约有80%仅能被LIF控制。对于LIF的治疗，LIF的效果出现在血液中LIF升高后的几分钟到6小时的不同时间。此外，CD9的表达和功能分析表明，CD9基因和蛋白水平在着床期升高，并在受精卵移植的CD9 KO小鼠中观察到着床抑制。采用慢病毒载体将AP和ZAP基因导入小鼠体内，并对其表达情况进行了验证

The molecule, which is available for embryonic implantation in mice, has not been discovered without the leukemia inhibitory factor (LIF). So, the molecular dynamics in the implantation was analysed by microarray technology using the LIF KO and the delayed implantation mice. The microarray analysis in this study used MG_U74Av2 array (Affymetrix Co.), which detected the 12,489 genes. We detected the repression of gene expression in 50 genes 4 days after pregnancy in the LIF KO and delayed implantation mice. Next, mice with delayed implantation were produced, and a role of LIF at the implantation period was clarified to be essential for the event. About 80% of the selected 50 genes could be controlled by only LIF. As for LIF treatment, the effects of LIF were arisen in various times from several minutes to 6 hours after increase of LIF in the blood. Furthermore, expression and functional analyses of CD9 revealed that CD9 was increased in gene and protein levels at the implantation period and the inhibition of implantation was observe in CD9 KO mice transplanted with fertilized eggs. As fox gene transfer technology using Lentivirus vector, AP and ZAP genes were introduced in mice and the expressions of these genes were confirmed