Rotenone-Induced Parkinsonism

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Rotenone (0, 1.5, 2.0, 2.5 mg/kg/day) was infused with Alzet minipumps into the right jugular vein for 4 weeks. The impairment of the nigrostriatal pathway was evaluated by spontaneous motor activities, striatal dopamine contents and immunohistochemistry of nigral and striatal neurons by using antibodies against tyrosine hydroxylase and ct-synuclein. The increase in the body weight of the rotenone-infused rats was significantly smaller than that of the vehicle-infused ones. The survival rate of each group was 100, 90, 80 and 45%, respectively. The spontaneous activities were significantly deceased in the 2.5 mg group as compared with the vehicle group at the 3rd week but returned normal at the 4th week. The other groups showed no statistically significant changes in the spontaneous activities. There were no significant changes in morphological evaluations. We conclude the rotenone model that was originally reported by Betarbet et al. (2000) to be substantially unreliable.