Rotenone-Induced Parkinsonism

鱼藤酮诱发的帕金森病

• 批准号: 14570088
• 负责人: NAKAKI Toshio
• 金额: $ 1.92万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570088/
• 关键词: rotenone; substantia nigra; tyrosine hydroxylase; α-synuclein; neurodegeneration; Parkinson's Disease; dopamine; Alzet minipump

Rotenone (0, 1.5, 2.0, 2.5 mg/kg/day) was infused with Alzet minipumps into the right jugular vein for 4 weeks. The impairment of the nigrostriatal pathway was evaluated by spontaneous motor activities, striatal dopamine contents and immunohistochemistry of nigral and striatal neurons by using antibodies against tyrosine hydroxylase and ct-synuclein. The increase in the body weight of the rotenone-infused rats was significantly smaller than that of the vehicle-infused ones. The survival rate of each group was 100, 90, 80 and 45%, respectively. The spontaneous activities were significantly deceased in the 2.5 mg group as compared with the vehicle group at the 3rd week but returned normal at the 4th week. The other groups showed no statistically significant changes in the spontaneous activities. There were no significant changes in morphological evaluations. We conclude the rotenone model that was originally reported by Betarbet et al. (2000) to be substantially unreliable.

鱼藤酮（0，1.5，2.0，2.5毫克/公斤/天）注入Alzet微型泵到右颈静脉4周。黑质纹状体通路的损害通过自发运动活动、纹状体多巴胺含量和黑质和纹状体神经元的免疫组织化学使用抗酪氨酸羟化酶和ct-突触核蛋白的抗体来评估。输注鱼藤酮的大鼠体重的增加显著小于输注溶剂的大鼠。各组的存活率分别为100%、90%、80%和45%。与溶剂组相比，2.5 mg组在第3周的自发活动显著减少，但在第4周恢复正常。其他组的自发活动无统计学显著性变化。形态学评价无显著变化。我们得出结论，Betarbet等人（2000）最初报告的鱼藤酮模型基本上是不可靠的。

项目成果

Suzuki, E.et al.: "Plasma nitrate levels in deficit vs nondeficit forms of schizophrenia"Rev Psychiatr Neurosci. 28. 288-292 (2003)

Suzuki, E.等人：“精神分裂症的血浆硝酸盐水平缺乏与非缺乏形式”Rev Psychiatr Neurosci。

Ohashi K., Naruto M., Nakaki T., Sano E.: "Identification of interleukin-8 converting enzyme as cathepsin L"Biochern. Biochim. Acta. 1649. 30-39 (2003)

Ohashi K.、Naruto M.、Nakaki T.、Sano E.：“将白细胞介素 8 转换酶鉴定为组织蛋白酶 L”Biochern。

Suzuki, E.et al.: "Long-term imipramine treatment increases nitrate levels in the rat hypothalamus"Cell.Mol.Neurobiol.. 23. 953-962 (2003)

Suzuki, E.等人：“长期丙咪嗪治疗会增加大鼠下丘脑中的硝酸盐水平”Cell.Mol.Neurobiol.. 23. 953-962 (2003)

Suzuki, E et al.: "Long-term imipramine treatment increase nitrate levels in the rat hypothalamus"Cell.Mol.Neurobiol.. 23. 953-962 (2003)

Suzuki, E 等人：“长期丙咪嗪治疗会增加大鼠下丘脑中的硝酸盐水平”Cell.Mol.Neurobiol.. 23. 953-962 (2003)

Ohashi, K.et al.: "Identification of interleukin-8 converting enzyme as cat hepsin L"Biochem.Biochim.Acta. 1649. 30-39 (2003)

Ohashi, K.等人：“将白细胞介素 8 转化酶鉴定为猫 hepsin L”Biochem.Biochim.Acta。