Implications of nitrocathecholamines in Parkinson's disease

硝基儿茶酚胺对帕金森病的影响

• 批准号: 12680760
• 负责人: NAKAKI Toshio
• 金额: $ 2.3万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680760/
• 关键词: nitric oxide; catecholamine; neurodegenation; Parkinson's disease; hydrogen peroxide; パーキンソン病; ドパミン; ノルエピネフリン

It was investigated whether or not 6-nitronorepinephrine increased hydrogen peroxide production. 6-Nitronorepinephrine increased it in rat vascular endothelial cells ; at 0 μM, 0-0.2 nmol/60 min/10^5 cells ; 0.3μM, 1-2 nmol/60 min/10^5 cells. Further inceases in the amine concentration was inappropriate because the color of the amine interfered the hydrogen peroxide assay.Possible apoptosis-eliciting activities of 6-nitronorepinephrine was tested with use of SH-SY5Y cells. Apoptosis was evaluated by released histone from mucleosomes. The amine at a concentration of 300 μM induced apoptosis. 6-1OHDA, a control compound, induced apoptosis at concentrations of 30 and 300 μM.A neurotoxic pesticide, glufosinate ammonium, was found to be able to stimulate nitric oxide production in rat brain and to induce convulsion. HMDA receptor antagonists inhibited those response.

研究了6-硝基肾上腺素是否增加过氧化氢的产生。6-在大鼠血管内皮细胞中，硝诺啡可增加其活性; 0 μM时，0-0.2 nmol/60 min/10^5个细胞; 0.3μM时，1-2 nmol/60 min/10^5个细胞。进一步增加胺的浓度是不合适的，因为胺的颜色干扰过氧化氢测定。用核小体释放组蛋白的方法评价细胞凋亡。浓度为300 μM的胺诱导细胞凋亡。对照化合物6- 1 OHDA在30和300 μM浓度下诱导细胞凋亡。发现神经毒性杀虫剂草铵膦铵能够刺激大鼠脑中一氧化氮的产生并诱导惊厥。HMDA受体拮抗剂抑制这些反应。

项目成果

Matsumura N: "Glufosinate ammonium induces convulsion through N-methyl D-aspartate receptors in mice"Neurosci. Lett. 304. 123-125 (2001)

Matsumura N：“草铵膦通过 N-甲基 D-天冬氨酸受体诱导小鼠惊厥”Neurosci。

中木敏夫: "NOと利尿ホルモン"蛋白質核酸酵素. 45. 1119-1124 (2000)

Toshio Nakagi：“NO 和利尿激素”蛋白质核酸酶。45。1119-1124 (2000)

Matsumura N: "Glufosinate ammonium induces convulsion through N-methyl D-aspartate receptors in mice"Neurosci.Lett. 304. 123-125 (2001)

Matsumura N：“草铵膦通过 N-甲基 D-天冬氨酸受体诱导小鼠惊厥”Neurosci.Lett。

Suzuki E., Yagi G., Nakai T., Kanba S. and Asai M.: "Elevated plasma nitrate levels in depressive states"J. Affect Disord. 63. 221-224 (2001)

Suzuki E.、Yagi G.、Nakai T.、Kanba S. 和 Asai M.：“抑郁状态下血浆硝酸盐水平升高”J。

Nakaki T.: "Soluble guanylyl cyclase"Nipon Rinsho. 58. 230-234 (2000)

Nakaki T.：“可溶性鸟苷酸环化酶”Nipon Rinsho。