Correlation between phenotypic expression and genetic changes of colorectal carcinomas, in evaluation of their biological behaviors

结直肠癌表型表达与遗传变化的相关性及其生物学行为的评估

• 批准号: 14570148
• 负责人: YAO Takashi
• 金额: $ 1.54万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570148/
• 关键词: CD10; MUC2; gastric mucin; mixed phenotype; microsatellite instability; マイクロサテライト不安定性; 大腸癌; Gastric mucin; human gastric mucin; 肝転移; adenoma-carcinoma sequence; de novo

With immunohistochemical methods using antibodies for human gastric mucin(HGM), MUC2 and CD10,we have been analyzed the correlation between the phenotypic expression and the progression, biological behavior and genetic changes of colorectal epithelial tumors. The following results have been found in the early period of this study.1)The phenotypic expression pattern of adenoma-carcinoma sequence was different from that of de nove carcinoma.2)Mixed phenotype was more frequently present in villous tumors, mutinous carcinomas and carcinoma arising in inflammatory bowel disease than in ordinal colorectal carcinomas.3)CD10 expression was more frequently present in carcinoma with liver metastasis and/or venous invasion than in ordinal colorectal carcinomas.Based on the above results, further studies have been carried out. In carcinomas arising from villous tumor and inflammatory bowel disease, microsatellite instability(MSI) was more frequently detected by immunohistochemical stains using antibodies for hMLH1 and hMSH2 and polymerase chain reaction(PCR) method using microsatellite markers than in ordinal colorectal carcinomas. In colorectal carcinoma of young patients (under 40), the association between mixed phenotype and MSI was also suggested.In colorectal carcinomas with liver metastasis, CD44v6,TGF-alpha and VEGF were more frequently expressed than in ordinal ones, however, there was no correlation between CD10 and their factors.

应用抗人胃粘蛋白(HGM)、MUC2和CD10的免疫组织化学方法，分析了表型表达与大肠上皮肿瘤的进展、生物学行为和遗传变化的关系。在本研究的早期阶段发现：1)腺瘤-癌序列的表型表达模式不同于新发现的结直肠癌。2)混合表型在绒毛状癌、突变癌和炎症性肠病引起的癌中比在序贯结直肠癌中更常见。3)CD10在有肝转移和/或静脉侵犯的结直肠癌中的表达比在序贯结直肠癌中更常见。在绒毛状癌和炎症性肠病的癌组织中，微卫星不稳定性(MSI)的发生率高于序贯结直肠癌。在有肝转移的结直肠癌中，CD44v6、转化生长因子-α和血管内皮生长因子的表达明显高于顺行性结直肠癌，而CD10与其相关因素之间无相关性。

项目成果

Interleukin 1β enhances invasive ability of gastric carcinoma through nulcear Factor-kB activation.

白细胞介素1β通过核因子-kB激活增强胃癌的侵袭能力。

• 发表时间: 2004
• 期刊: Clin Cacer Res 10
• 作者: Yamanaka N;Morisaki T;Nakashima H;Tasaki A;Kubo M;Kuga H;Nakahara C;Nakamura K;Noshiro H;Yao T;Tsuneyoshi M;Tanaka M;Katano M.
• 通讯作者: Katano M.

Oya M, Yao T, Nakamura T, Nakanishi K, Tsuneyoshi M: "Intestinal phenotypic expression of gastric depressed adenomas and the surrounding mucosa"Gastric Cancer. 6. 179-184 (2003)

Oya M，Yao T，Nakamura T，Nakanishi K，Tsuneyoshi M：“胃凹陷性腺瘤和周围粘膜的肠表型表达”胃癌。

Nishiyama K, Yao T, Yonemasu H, et al.: "Overexpression of p53 protein and point mutation of K-ras genes in primary carcinoma of the small intestine"Oncology Reports. 9(2). 293-300 (2002)

Nishiyama K、Yao T、Yonemasu H 等人：“原发性小肠癌中 p53 蛋白的过度表达和 K-ras 基因的点突变”肿瘤学报告。

Malignant transformation of gastric hyperplastic polyps: Alteration of phenotypes, proliferative activity, and p53 expression

• DOI: 10.1053/hupa.2002.126874
• 发表时间: 2002-10-01
• 期刊: HUMAN PATHOLOGY
• 影响因子: 3.3
• 作者: Yao, T;Kajiwara, M;Tsuneyoshi, M
• 通讯作者: Tsuneyoshi, M

Immunohistochemical characteristics of duodenal adenomas in familial adenomatous polyposis with special reference to cell kinetics.

家族性腺瘤性息肉病十二指肠腺瘤的免疫组织化学特征，特别参考细胞动力学。

• 发表时间: 2005
• 期刊: Human Pathology 36
• 作者: Esaki M;Matsumoto T;Yao S;Nakamura S;Hirahashi M;Yao T;Iida M.
• 通讯作者: Iida M.