Correlation between phenotypic expression and genetic changes of colorectal carcinomas, in evaluation of their biological behaviors

采用免疫组织化学方法，利用人胃粘蛋白（HGM）、MUC2和CD10抗体，分析了表型表达与结直肠上皮肿瘤的进展、生物学行为和遗传变化的相关性。在本研究的前期，我们发现了以下结果。1)腺瘤-癌序列的表型表达模式与非腺癌不同。2)混合表型在绒毛瘤、叛变癌和炎症性肠病引起的癌中比在普通结直肠癌中更常见。3)CD10在肝转移和/或静脉浸润癌中的表达高于普通结直肠癌。在以上结果的基础上，进行了进一步的研究。在绒毛状肿瘤和炎症性肠病引起的癌中，使用hMLH1和hMSH2抗体的免疫组织化学染色和使用微卫星标记的聚合酶链反应（PCR）方法检测微卫星不稳定性（MSI）的频率高于普通结直肠癌。在年轻结直肠癌患者（40岁以下）中，混合表型与MSI之间也存在关联。在肝转移结直肠癌中，CD44v6、tgf - α和VEGF的表达频率高于普通结直肠癌，而CD10与这些因素之间没有相关性。

With immunohistochemical methods using antibodies for human gastric mucin(HGM), MUC2 and CD10,we have been analyzed the correlation between the phenotypic expression and the progression, biological behavior and genetic changes of colorectal epithelial tumors. The following results have been found in the early period of this study.1)The phenotypic expression pattern of adenoma-carcinoma sequence was different from that of de nove carcinoma.2)Mixed phenotype was more frequently present in villous tumors, mutinous carcinomas and carcinoma arising in inflammatory bowel disease than in ordinal colorectal carcinomas.3)CD10 expression was more frequently present in carcinoma with liver metastasis and/or venous invasion than in ordinal colorectal carcinomas.Based on the above results, further studies have been carried out. In carcinomas arising from villous tumor and inflammatory bowel disease, microsatellite instability(MSI) was more frequently detected by immunohistochemical stains using antibodies for hMLH1 and hMSH2 and polymerase chain reaction(PCR) method using microsatellite markers than in ordinal colorectal carcinomas. In colorectal carcinoma of young patients (under 40), the association between mixed phenotype and MSI was also suggested.In colorectal carcinomas with liver metastasis, CD44v6,TGF-alpha and VEGF were more frequently expressed than in ordinal ones, however, there was no correlation between CD10 and their factors.