Study on the effect of arsenic exposure during the embryonic on the behavioof younger generation

胚胎期砷暴露对年轻一代行为影响的研究

• 批准号: 14570295
• 负责人: ITOH Toshihiro
• 金额: $ 2.11万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570295/
• 关键词: Inorganic arsenic; behavior; Drinking water; development; Trivalent arsenite; Pentavalent arsenate

We investigated the effect of arsenic exposure from the before embryonic on the development of central nervous system of the child. C57B1/6 mouse were exposed trivalent arsenite(As (III)) and pentavalent arsenate(As(v)) in low-level(10ppm) or high-level(100ppm) in drinking-water, and the arsenic exposure have continued to 3 generations. The behavioral development test in the early stage of growth, and Open-field test and T-type water maze test after the growth were carried out the animals in 3 generation(F2). After the behavioral tests, immunological function was tested using lymphocyte of the lien.We observed different effect of long-term inorganic arsenic exposure for the development of postnatal behavior between As(III) and As(V). Little effects of the As exposure on the behavioral development test of the mouse were observed in the As(III) animals, but delay of development of the reflection and motor function was observed in the As(V) animals. In addition, some effects on the emotional reaction after the growth were also observed in the As exposed animals. It is not known the reason that we obtained different results for their behavior between long term exposure of As(III) and As(V) animals. But, it is necessary to clarify the mechanism for these causes in future.

本文探讨了砷暴露对儿童中枢神经系统发育的影响。C57 B1/6小鼠经饮水暴露于低浓度（10 ppm）和高浓度（100 ppm）的三价砷（As（III））和五价砷（As（V）），连续暴露3代。对3代（F2）动物进行生长早期行为发育试验、生长后旷场试验和T型水迷宫试验。行为学检查结束后，用淋巴细胞免疫功能测定法，观察长期无机砷暴露对As（III）和As（V）大鼠出生后行为发育的不同影响。在As（III）动物中观察到As暴露对小鼠行为发育测试的影响很小，但在As（V）动物中观察到反射和运动功能的发育延迟。此外，在砷暴露动物中还观察到对生长后情绪反应的一些影响。长期暴露于As（III）和As（V）的动物，其行为结果不同，原因尚不清楚。但是，将来有必要阐明这些原因的机制。