Use of urinary bilirubin oxidative metabolites as a psychological stress-marker.

一些作者认为，心理应激会导致活性氧(ROS)的产生。一些研究支持胆红素在体内具有抗氧化作用。然而，目前尚不清楚心理应激诱导的ROS是否会在体内引起胆红素氧化。我们调查了暴露在心理压力下的受试者尿液中生物吡喃的浓度是否增加，这是一种胆红素氧化代谢产物。60名在制药公司工作的健康男性志愿者被分为三组，一组没有参加会议，一组参加了会议但没有发表演讲，三组参加了会议并在公司高管在场的情况下发表了演讲。在第三组受试者在会议上发言后进行主观应激评分(自评分数)。第二天收集尿液。用酶联免疫吸附试验测得的生物吡喃浓度与尿肌酐浓度归一化。与I组和II组相比，III组的生物吡咯浓度显著升高(P<0.01和P<0.05)。此外，在第三组中，生物吡喃浓度与自评应激分数呈正相关(r=0.53，p&lt；0.01)。这些发现表明，情绪刺激与人尿中胆红素氧化代谢产物的增加有关，生物吡喃可能是心理压力的有用标志。根据这些关于心理应激、氧化应激和胆红素作为抗氧化剂之间的可能关系的报道，我们探讨了心理应激导致氧化条件的可能性，以及随后胆红素与ROS反应产生的生物吡喃尿液浓度增加的可能性。

Some authors have suggested that psychological stress induces the production of reactive oxygen species(ROS). Some studies have supported that bilirubin exerts anti-oxidative effects in vivo. However, it is not known whether ROS induced by psychological stress provoke bilirubin oxidation in vivo. We investigated if the concentration of biopyrrin, a bilirubin oxidative metabolite, increased in urine from subjects exposed to psychological stress. Sixty healthy male volunteers working in a pharmaceutical company were divided into a Group I which did not attend a conference, a Group II which attended a conference but did not deliver a speech, and a Group III which attended a conference and delivered speeches in the presence of the company executives. Subjective stress was scored (self-rating score) after subjects in Group III delivered their speeches at the conference. Urine was collected on the next day. The biopyrrin concentrations, as measured by enzyme-linked immunosorbent assay, were normalized to the urinary concentration of creatinine. The concentration of biopyrrin in Group III was significantly higher compared to that in Groups I and II (p<0.01 and p<0.05, respectively). Furthermore, in Group III, the concentration of biopyrrin correlated with the self-rating stress score (r=0.53, p<0.01). These findings suggest that emotional stimuli are associated with an increase in the oxidative metabolites of bilirubin in human urine, and that biopyrrins could be useful markers of psychological stress. Based on these reports on the possible relationship among psychological stress, oxidative stress and bilirubin as the antioxidant, we examined the possibility that psychological stress contributed to the oxidative conditions, and the subsequent increase of the urinary concentration of biopyrrins produced by the reaction of bilirubin with ROS.