Immunohistologial study on the expression of developmental intestinal epithelial antigens in embryogenesis and oncogenesis.

胚胎发生和肿瘤发生中发育肠上皮抗原表达的免疫组织学研究。

基本信息

批准号：
14570499
负责人：
OKADA Yoshio
金额：
$ 2.18万
依托单位：
Okayama Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

1.Monoclonal antibodies that can be used for immunohistostaining of the formalin-fixed tissues were established to study the expression of the developmental antigens in rat embryonal and cancerous intestinal epithelia. Using the synthetic oligopeptides, mouse monoclonal antibodies against sucrase-isomaltase, MUC2 tandem and non-tandem repeat antigens, Cdx1, Cdx2, and Musashi1 were established.2.Expression of developmental intestinal epithelial antigens was studied immunohistochemically using the newly established monoclonal antibodies and the fixed tissues of embryonal rat intestines. Cdx2 was found to be expressed in the nuclei of endodermal cells at day 11 pc, but Cdx1 was first detected at day 15 pc. As the intestinal villi developed, Cdx1 and Cdx2, respectively, tended to localize in the intervillous endoderms and the villous epithelia. Although both of MUC2 and sucrase-isomaltase were synthesized and expressed before birth, the former expression preceded three days of the latter expression that was first detected at day 19 pc. The expression of Cdxs was not simply correlated with those of MUC2 and sucrase-isomlatase.3.Expression of apomucins, the blood group-related carbohydrate antigens and Cdx2 in the AOM-induced rat intestinal tumors was studied. Their expressions were generally suppressed in the tumors, though MUC5AC and H-antigen were observed to be expressed in the tissues not expressing them normally. Quantitative, but not qualitative, differences in the expression existed between the adenomas and the cancers. Although B-antigen expressed in the proximal colon might be carried by MUC5AC, there was no apparent association between the expressions of apomucins, the carbohydrate antigens and Cdx2. Expression of Cdx2 was almost completely suppressed in the AOM tumors.

1.建立了可用于福尔马林固定组织的免疫组织的单克抗体，以研究大鼠胚胎和癌性肠上皮中的发育抗原的表达。使用合成寡肽，对抗硫酶 - 异构酶，MUC2串联和非串联重复抗原，CDX1，CDX2和Musashi1的小鼠单克隆抗体进行表达。肠。发现CDX2在第11天PC中在内皮细胞的核中表达，但在第15天首先检测到CDX1。随着肠绒毛的发展，CDX1和CDX2分别倾向于本地化的内胚层和绒毛上皮。尽管MUC2和Sucrase-异构酶均在出生前合成并表达，但前者的表达在后一种表达的三天之前首次在第19天PC上检测到。 CDX的表达不仅与MUC2和Sucrase-Isomlatase的表达相关。3。Apomucins在AOM诱导的大鼠肠道肿瘤中的表达，与血型相关的碳水化合物抗原和CDX2相关。尽管观察到在不正常表达它们的组织中表达的MUC5AC和H-抗原，但它们的表达通常在肿瘤中受到抑制。腺瘤和癌症之间存在定量但不是定性的差异。尽管在近端结肠中表达的B抗原可能是MUC5AC携带的，但阿哌辛蛋白，碳水化合物抗原和CDX2的表达之间没有明显的关联。在AOM肿瘤中，CDX2的表达几乎被完全抑制。