Immunohistologial study on the expression of developmental intestinal epithelial antigens in embryogenesis and oncogenesis.

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1.Monoclonal antibodies that can be used for immunohistostaining of the formalin-fixed tissues were established to study the expression of the developmental antigens in rat embryonal and cancerous intestinal epithelia. Using the synthetic oligopeptides, mouse monoclonal antibodies against sucrase-isomaltase, MUC2 tandem and non-tandem repeat antigens, Cdx1, Cdx2, and Musashi1 were established.2.Expression of developmental intestinal epithelial antigens was studied immunohistochemically using the newly established monoclonal antibodies and the fixed tissues of embryonal rat intestines. Cdx2 was found to be expressed in the nuclei of endodermal cells at day 11 pc, but Cdx1 was first detected at day 15 pc. As the intestinal villi developed, Cdx1 and Cdx2, respectively, tended to localize in the intervillous endoderms and the villous epithelia. Although both of MUC2 and sucrase-isomaltase were synthesized and expressed before birth, the former expression preceded three days of the latter expression that was first detected at day 19 pc. The expression of Cdxs was not simply correlated with those of MUC2 and sucrase-isomlatase.3.Expression of apomucins, the blood group-related carbohydrate antigens and Cdx2 in the AOM-induced rat intestinal tumors was studied. Their expressions were generally suppressed in the tumors, though MUC5AC and H-antigen were observed to be expressed in the tissues not expressing them normally. Quantitative, but not qualitative, differences in the expression existed between the adenomas and the cancers. Although B-antigen expressed in the proximal colon might be carried by MUC5AC, there was no apparent association between the expressions of apomucins, the carbohydrate antigens and Cdx2. Expression of Cdx2 was almost completely suppressed in the AOM tumors.