Infectious disease, and hepatic and gastrointestinal disease in Japan and China

鉴于人肝二肽基肽酶Ⅱ（DPP Ⅱ）的性质尚不清楚，本文对DPP Ⅱ的分子量、动力学参数、氨基酸序列和cDNA结构等进行了测定。然而，由于获得人肝脏并不容易，作为初步实验，我们尝试了从人精浆中纯化DPP II。用DEAE-Sephacel、Superdex 200和Phenyl-Sepharose进行SDS-PAGE纯化。并测定了其分子量、N端氨基酸序列及在前列腺组织中的免疫组织化学定位。纯化酶的Mr为140，000。该酶优先切割底物如Ala-、Phe-、Leu-和Lys-Ala-MCAs。该蛋白在前列腺腔细胞中呈强染色。此外，该酶的氨基酸序列和cDNA结构表明，人精浆中的DPP II是一种氨肽酶，即丙氨酰氨肽酶（AAP） ...更多信息 .本课题组在前期实验的基础上，结合上述研究结果，拟从人肝中分离纯化该酶，并在中日研究组的合作下对其进行表征。口腔中嗜温菌种类多，数量多，分离致病菌难度大。本研究将日本人感染与中国人感染进行比较，以建立有效的药物治疗方法。样本收集自Chirlin大学医院正在接受感染治疗的患者。结果：葡萄球菌属、肠球菌属、链球菌属、消化球菌属、消化链球菌属、棒状杆菌属、乳杆菌属、诺卡氏菌属、齿菌属、细菌性线虫属。用生物测定法测定了这些细菌对抗生素的敏感性。敏感抗生素为头孢菌素和青霉素，与我科以往的检测结果相似。少

On the reason that little is known the properties of human liver dipeptidyl peptidase II (DPP II), we tried to determine the characteristics incliding molecular weight, kinetic parameters, amino acid sequence and cDNA structure. However, since it is not so easy to get human liver, as a preliminary experiment, we tried the purification of DPP II from human seminal plasma. The DPP II was purified to homogenity on SDS-PAGE by DEAE-Sephacel, Superdex 200 and Phenyl-Sepharose. We also determined its molecular weight (Mr), N-terminal amino acid sequence and its immunohistochemical localization in human prostatic gland. The Mr of the purified enzyme was 140,000. The enzyme preferentially clevaged the substrates such as Ala-, Phe-, Leu- and Lys-Ala-MCAs. The protein was strongly stained the luminal cells in prostatic gland. Furthermore, the amino acid sequence and cDNA structure of the enzyme indicated that DPP II in human seminal plasma is an aminopeptidase, namely alanyl aminopeptidase (AAP) … More . On the basis of our preliminary experiment using human liver and the above data, we are also planing to purify the enzyme from human liver, and to characterize under the colaboration between Japanese and Chinese research groups.It is very difficult to isolate of pathogenic bacteria because of there are many number of and many sort of mesophilic bacteria in mouth. In this study, we compared with the infection in Japanese and the infection in Chinese to establish a effective medicinal therapy.The samples were collected from the patients who were undertreating for infection in Chirlin University Hospital. The samples were cultured and tested by basteriological examination.In result, Staphylococcus, Enterococcus, Streptococcus, Peptococcus, peptostreptococcus, Corynebacterium, Lactobacillus, Norcardia, Odontomyces and Bacterionema were harvested. Susceptibility of these bacteria to some antibiotics was tested by bioassay. Then, susceptible antibiotics were Cephalosporin and Penicillin.These results were very similar to our results which were examined in our department before. Less