The expression of HSP47 and the antisense therapy in the atherosclerosis animal model

动脉粥样硬化动物模型中HSP47的表达及反义治疗

• 批准号: 14570678
• 负责人: OZONO Yoshiyuki
• 金额: $ 1.73万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570678/
• 关键词: HSP47; atherosclerosis; ADOE knockout mouse; antisense therapy; ApoEノックアウトマウス; 免疫組織染色; コラーゲン; リモデリング

The relationship between the progression of atherosclerosis and HSP47. was examined in ApoE knockout mouse as the animal model of atherosclerosis. The arterial walls of he animals were examined about the development of atherosclerosis at the aging ranging from 5 weeks to 28 weeks. Gross examination by dissection microscopy revealed the acculation of macrophage, the formation of atherom, foam cell lesion after 22 weeks mouse.The HSP47 expression in the arterial walls in the ApoE knockout mouse were assessed by immunostaining (Anti HSP 47 monoclonal antibody : Stressgen Biotechnolpgies Corp, Victoia BC, Canada). The HSP47 expression and the collagenI were evident in the arteriosclerotic lesions after 22weeks AopE knockout mouse.The antisense ODNs against HSP47 was administrated in the vein in the ApoE knockout mouse tail in order to suppress the progression of atherosclerosis.But the uptake of this antisense was not efficient for the purpose of atherosclerosis treatment in this animal model. The elnew strategy for the HSP47 antisense therapy in this model.

热休克蛋白47与动脉粥样硬化进展的关系。在ApoE基因敲除小鼠中作为动脉粥样硬化的动物模型进行检查。在5周龄至28周龄的动物中，观察动脉壁的动脉粥样硬化的发展。22周后，解剖显微镜检查显示巨噬细胞聚集，动脉粥样硬化形成，泡沫细胞病变。通过免疫染色（抗HSP 47单克隆抗体：Stressgen Biotechnolpgies Corp，Victoia BC，Canada）评估ApoE敲除小鼠动脉壁中HSP 47的表达。AopE基因敲除小鼠22周后动脉粥样硬化病变中可见HSP 47表达和I型胶原的表达，在ApoE基因敲除小鼠尾静脉注射HSP 47反义寡核苷酸可抑制动脉粥样硬化的进展，但在该动物模型中，HSP 47反义寡核苷酸的摄取对动脉粥样硬化的治疗无效。HSP47反义治疗的新策略。

项目成果

西野友哉, 大園恵幸他: "Antisense oligonucleotid against collagen binding stress protein HSP47 suppress Peritoneal fibrosis in rats"Kidney International. 64. 887-896 (2003)

Tomoya Nishino、Keiyuki Ozono 等人：“抗胶原结合应激蛋白 HSP47 的寡核苷酸抑制大鼠腹膜纤维化”《肾脏国际》64. 887-896 (2003)。

Tomoya Nishino, Yoshiyuki Ozono et al.: "Antisense oligonucleotides against-binding stress protein HSP47 suppress peritoneal fibrosis in rats."Kidney Internatinal. 64. 887-896 (2003)

Tomoya Nishino、Yoshiyuki Ozono 等人：“针对结合应激蛋白 HSP47 的反义寡核苷酸可抑制大鼠腹膜纤维化。”Kidney Interatinal。