THE STUDY OF THE THERAPEUTIC MECHANISMS OF ANTIDEPRESSANT ON NEURONAL REMODELING

点击翻译按钮获取中文摘要

We have previously identified 200 cDNA fragments as antidepressant related genes (ADRG) from rat frontal cortex and hypothalamus using differential cloning technique. In the present study, we continued this screening in rat hippocampus and identified total of 707 cDNA and developed the ADRG microarray. More recently, we used ADRG microarray to search for s**e genes commonly induced after chronic antidepressant treatment and repeated ECT. ECT is another therapy for depressive patients that is widely used, particularly in the treatment of patients who are resistant to antidepressant medications. Interestingly, we found some genes related to neurite outgrowth, were commonly induced or decreased after chronic antidepressant treatment and repeated ECT. Thus, the unknown candidate ADRG genes may be thought that belong to the function related to the neurite outgrowth. ADRG#102 and ADRG#123 were subcloned to GFP expression vector and transfected to NGF-induced differentiated PC12 cels. Measurement of the morphological changes demonstrated that the mean length of the longest neurite in each cell and the total length of neurites per cell was significantly shorter in the transfectants, respectively. In addition, the mean neurite number per cell was also significantly smaller in the transfectants. One of the long-term therapeutic action of antidepressant may be related to the changes of both functional and structural neuroplasticity.