Inleetin and pathogenetic role of lymphotropic viruses in hematological malignancies.

1.EB病毒(EBV)与多种淋巴增生性疾病有关。脓胸相关淋巴瘤(PAL)与EBV密切相关，但这种病毒如何促进淋巴瘤的发展是已知的。PAL为病毒相关淋巴肿瘤的研究提供了一个很好的模型，建立PAL来源的细胞系是可取的。在本研究中，我们成功地建立了一个新的PAL细胞系。该细胞系在特定的部位意外地具有整合的EBV。病毒整合事件被认为是为了增强插入部位的染色体不稳定性。该细胞系代表了第一个在PAL中整合EBV的例子，并可能使研究整合病毒感染在淋巴癌发生中的作用。2.EBV和其他疾病：(1)已知在10-15%的胃癌中观察到单克隆性EBV感染。我们首次描述了一例合并胃淋巴瘤和成人T细胞白血病/淋巴瘤的病例，其中检测到EBV感染的细胞。3人类疱疹病毒6型(HHV-6)与疾病：病原学和成人发病斯蒂尔病是已知的。我们认为HHV-6感染再激活可能是成人斯蒂尔病的一个致病因素。4.HHV-8与疾病：在美国患者中，HHV-8与原发性肺动脉高压(PPH)之间存在因果关系。然而，我们的研究表明，在日本患者中，HHV-8与PPH没有相关性。5其他病毒：两个美国小组报告说，在大约40%的淋巴瘤中检测到猿猴病毒40(SV40)。在这项研究中，我们在日本淋巴增生性疾病患者的样本中寻找SV40。在178个样本中只有3个(1.7%)检测到该病毒，这表明SV40不太可能在日本患者中发挥致病作用。

1.Epstein-Barr virus(EBV) is associated with several lymphoproliferative diseases. Pyothorax-associated lymphoma(PAL) is closely associated with EBV, but how this virus contributes to the development of the lymphoma is known. PAL would provide an excellent model for investigation of virus-associated lymphomagenesis, and establishment of PAL-derived cell lines is desirable. In this study, we have successfully established a novel PAL cell line. The cell line unexpectedly possessed integrated EBV at a specific site. The viral integration event is suggested to enhance a chromosomal instability at the insertion site. The cell line represents the first example of EBV integration in PAL and could enable the study of the role of integrated viral infection in lymphomagenesis.2.EBV and other diseases : (1)It is known that monoclonal EBV infection is observed in 10-15% of gastiric carcinoma. We described for the first time a case of concomitant gastric lymphoma and adult T-cell leukemia/lymphoma in which EBV infected cells were detected. (2)We also proposed that reactivated EBV infection is associated with a subset of pure red cell aplasia.3 Human herpesvirus 6 (HHV-6) and diseases : The etiology and adult onset Still's disease is known. We suggested that reactivated HHV-6 infection could be a causative factor of adult onset Still's disease.4.HHV-8 and diseases : The causative relation between HHV-8 and primary pulmonary hypertension (PPH) has been suggested in USA patients. However, our study showed no association of HHV-8 with PPH in Japanese patients.5 Other virus : Two USA groups reported that simian virus 40 (SV40) was detected in about 40% of lymphoma. In this study, we looked for SV40 in samples from Japanese patients with lymphoproliferative disorders. The virus was detected in only 3 (1.7%) of 178 samples, suggesting that SV40 is unlikely to play a pathogenetic role in Japanese patients.