Inleetin and pathogenetic role of lymphotropic viruses in hematological malignancies.

1.eb病毒（EBV）与几种淋巴增生性疾病有关。脓胸相关淋巴瘤（PAL）与EBV密切相关，但这种病毒如何促进淋巴瘤的发展尚不清楚。PAL将为病毒相关淋巴瘤的研究提供一个很好的模型，建立PAL衍生细胞系是很有必要的。在这项研究中，我们成功地建立了一种新的PAL细胞系。该细胞系意外地在一个特定的位点拥有整合的EBV。病毒整合事件被认为增强了插入位点的染色体不稳定性。该细胞系是eb病毒在PAL中整合的第一个例子，可以用于研究整合病毒感染在淋巴瘤形成中的作用。EBV与其他疾病：(1)已知在10-15%的胃癌中有EBV单克隆感染。我们首次报道了一例合并胃淋巴瘤和成人t细胞白血病/淋巴瘤，其中检测到EBV感染细胞。(2)我们还提出，再激活的EBV感染与纯红细胞再生有关人类疱疹病毒6 （HHV-6）和疾病：病因学和成人发病斯蒂尔氏病是已知的。我们认为再次激活的HHV-6感染可能是成人发病斯蒂尔氏病的一个致病因素。HHV-8与疾病：HHV-8与原发性肺动脉高压（PPH）之间的因果关系已在美国患者中提出。然而，我们的研究显示在日本患者中HHV-8与PPH没有关联其他病毒：两个美国研究小组报告在大约40%的淋巴瘤中检测到类人猿病毒40 （SV40）。在这项研究中，我们在日本淋巴增生性疾病患者的样本中寻找SV40。178份样本中只有3份（1.7%）检测到该病毒，这表明SV40不太可能在日本患者中起致病作用。

1.Epstein-Barr virus(EBV) is associated with several lymphoproliferative diseases. Pyothorax-associated lymphoma(PAL) is closely associated with EBV, but how this virus contributes to the development of the lymphoma is known. PAL would provide an excellent model for investigation of virus-associated lymphomagenesis, and establishment of PAL-derived cell lines is desirable. In this study, we have successfully established a novel PAL cell line. The cell line unexpectedly possessed integrated EBV at a specific site. The viral integration event is suggested to enhance a chromosomal instability at the insertion site. The cell line represents the first example of EBV integration in PAL and could enable the study of the role of integrated viral infection in lymphomagenesis.2.EBV and other diseases : (1)It is known that monoclonal EBV infection is observed in 10-15% of gastiric carcinoma. We described for the first time a case of concomitant gastric lymphoma and adult T-cell leukemia/lymphoma in which EBV infected cells were detected. (2)We also proposed that reactivated EBV infection is associated with a subset of pure red cell aplasia.3 Human herpesvirus 6 (HHV-6) and diseases : The etiology and adult onset Still's disease is known. We suggested that reactivated HHV-6 infection could be a causative factor of adult onset Still's disease.4.HHV-8 and diseases : The causative relation between HHV-8 and primary pulmonary hypertension (PPH) has been suggested in USA patients. However, our study showed no association of HHV-8 with PPH in Japanese patients.5 Other virus : Two USA groups reported that simian virus 40 (SV40) was detected in about 40% of lymphoma. In this study, we looked for SV40 in samples from Japanese patients with lymphoproliferative disorders. The virus was detected in only 3 (1.7%) of 178 samples, suggesting that SV40 is unlikely to play a pathogenetic role in Japanese patients.