Plasma levels of ghrelin in anorexia nervosa patients

神经性厌食症患者血浆生长素释放肽水平

• 批准号: 14571076
• 负责人: SUZUKI Mari
• 金额: $ 1.15万
• 依托单位: National Graduate Institute for Policy Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571076/
• 关键词: Ghrelin; Aniorexia nervosa; Degradation; Anorrexis nervosa,; Helicobactor pylori; Body mass index; 神経性食欲不振症; グレリン; 摂食中枢; インスリン; 成長ホルモン; インスリン様成長因子-I; 血糖

Octanoylated ghrelin (1-28) (intact ghrelin) is rapidly degraded to desoctanoyl forms or smaller fragments (degraded ghrelin). Plasma levels of intact and degraded ghrelin were examined in 30 anorexia nervosa patients (AN) (body mass index (BMI) : 8.81-22.4 kg/m^2) and 16 age-matched healthy women using several assay methods. Plasma ghrelin levels measured by immunocomplex transfer-enzyme immunoassay (ICE-EIA) detecting specifically intact ghrelin in AN were lower than those of controls. Plasma ghrelin levels in AN by Active Ghrelin ELISA Kit advertised as specifically detecting intact ghrelin were not significantly different from controls. Levels of desoctanoyl ghrelin by Desacyl-Ghrelin ELISA, N-terminus ghrelin by Ghrelin Active RIA, and C-terminus ghrelin by Ghrelin Total RIA were significantly higher in AN than in controls, and displayed significant negative correlations with BMI.Plasma levels of intact ghrelin in AN vary from lower than to similar to those of controls. Degraded forms of ghrelin are elevated in AN.

辛酰化的Ghrelin(1-28)(完整的Ghrelin)被迅速降解为脱辛酰形式或更小的片段(降解的Ghrelin)。用多种方法检测了30例神经性厌食症(AN)患者(体重指数8.81~22.4 kg/m~2)和16例年龄匹配的健康女性血浆中完整的和降解的Ghrelin水平。免疫复合物转移酶免疫分析法(ICE-EIA)检测AN患者血浆Ghrelin水平低于正常对照组。活性Ghrelin ELISA试剂盒宣称能特异性检测完整Ghrelin的AN患者的血浆Ghrelin水平与对照组没有显著差异。用Desacyl-ghrelin EL ISA法检测AN患者血浆去辛酰化Ghrelin水平，用Ghrelin活性RIA法检测N端Ghrelin水平，用Ghrelin总RIA法测定C端Ghrelin水平，结果显示AN患者血浆完整Ghrelin水平低于或接近正常对照组，且与BMI呈显著负相关。在AN中，降解形式的Ghrelin升高。

项目成果

堀田眞理: "神経性食欲不振症"思春期学. 22. (2004)

Mari Hotta：“神经性厌食症”青春期研究。22。（2004）

堀田眞理他: "神経性食欲不振症の骨代謝"内分泌・糖尿病科. 14・6. 599-607 (2002)

Mari Hotta 等：“神经性厌食症的骨代谢”，内分泌和糖尿病系 14・6（2002 年）。

堀田眞理: "神経性食欲不振症"日本小児東洋医学会誌. 19. 61-68 (2003)

Mari Hotta：“神经性厌食症”日本小儿东方医学会杂志 19. 61-68 (2003)。

Mari Hotta et al.: "Low serum levels of estradiol can improve highly catabolic bone metabpolism in severely emaciated patients with anorexia nervosa"J Bone Miner Res. (submitted).

Mari Hotta 等人：“低血清水平的雌二醇可以改善患有神经性厌食症的严重消瘦患者的高分解代谢骨代谢”J Bone Miner Res。

Mari Hotta et al.: "A young female patient with anorexia nervosa complicated by mycobacterium Szulgai"International Journal of Eating Disorders. (in press). (2003)

Mari Hotta 等人：“一名年轻女性神经性厌食症并发分枝杆菌 Szulgai”《国际饮食失调杂志》。