A neuroendocrinological study for rats exposed to emotional stress as a model of anorexia nervosa.

对暴露于情绪压力的大鼠作为神经性厌食症模型的神经内分泌学研究。

基本信息

批准号：
06670980
负责人：
SUZUKI Mari
金额：
$ 1.22万
依托单位：
Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The chronic exposure to emotional stress using by a communications box system (1.5mA/sec, 2times/min, 1hr/time, 2times/day for 7day) significantly reduced total amount of food intake as well as body weight gain in Wister strain's rats. That stress also decreased blood sugar and increased plasma levels of ACTH and corticosterone. Rats chronically exposed to emotional stress showed increased locomotor activity in a familiar environment and decreased exploratory behaivior in a novel environment. The content of immunoreactive corticotropin-releasing factor (CRF) significantly increased in hypothalamus in rats chronically exposed to emothinal stress. It was observed by in situ hybridization that the emotinal stress for 5days induced CRF m RNA expression only in parvocellulr neurons of paraventricular nucleus in rats. The exposure to emotional stress increased noradrenaline release of paraventricular nucleus examined by intracerebral microdialysis. The increase of noradrenaline induced by emotional stress for 5min on the first day of the experiment did not change when the same stress was repeated on the 7th day, which indicated that desensitization of noradrenaline neurons dose not occur. These changes induced by chronic exposure to emotional stress were smaller than those by electric footshock.The emotional stress used in our study induced the similar findings in rats to those in patients with anorexia nervosa except for hypogonadism, which was accompanied by activation of CRF and noradrenaline releases in paraventricular nucleus.

长期使用通信盒系统（1.5ma/sec，2times/min，1hr/time，2times/day 7天的时间）的长期暴露于情绪压力，大大降低了食物摄入量的总量以及体重增加的大鼠。这种压力还降低了血糖并增加了血浆ACTH和皮质酮的水平。长期暴露于情绪压力的大鼠在熟悉的环境中显示运动活动增加，并在新型环境中降低了探索性的行为。在长期暴露于症状症状的大鼠下，下丘脑下丘脑中免疫反应性皮质激素释放因子（CRF）的含量显着增加。通过原位杂交观察到，5天的情绪应激仅在大鼠的旁脑核核的副细胞神经元中诱导CRF M RNA表达。暴露于情绪压力增加了脑肾上腺素释放的旁脑核的释放，这些核核通过了脑脑透析检查。当实验的第一天，在5分钟内由情绪应激诱导的去甲肾上腺素的增加没有改变，当时第七天重复相同的应激，这表明不发生去甲肾上腺素神经元剂量的脱敏。长期暴露于情绪压力引起的这些变化小于电动脚垫的变化。我们研究中使用的情绪压力诱导了与神经性厌食症患者的大鼠相似的发现，除了降低了神经性症患者，而伴有CRF的激活和甲状腺肿室中的甲肾上腺素释放。