Retrograde neuronal cell death in the facial nucleus after axotomy in the brainstem -alteration of MMPs expression, cell migration and axonal regrowth-

脑干轴索切除术后面核中的逆行神经元细胞死亡 -MMP 表达、细胞迁移和轴突再生的改变 -

• 批准号: 14571302
• 负责人: HASEGAWA Mitsuhiro
• 金额: $ 2.24万
• 依托单位: KANAZAWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571302/
• 关键词: MMPs; facial nerve injury; retrograde degeneration; Schwann cell; brainstem; facial nucleus; peripheral nerve transplantation

The aim of this study is to establish a model to investigate the unknown mechanism of retrograde neuronal cell death in the facial nucleus after axotomy at various lesions.In addition, the neuroprotective effects of autografted peripheral nerve tissues, and alteration of MMPs expression are investigated.The models include brainstem injury model ; the genu of the facial nerve tract in the brainstem is stereotactically transected, control injury model ; the brainstem near the facial nucleus is injured without transection of the facial nerve tract, distal injury model ; the facial nerve is cut at the stylomastoid foramen, proximal injury model ; the facial nerve is avuked at the stylomastoid foramen resulting in more proximal transection than the distal injury model, and transplanted model ; PNS autograft is transplanted to the injury site of the brainstem injury model.On day 7, compared with the contralateral side, the survival ratio of motoneurons of the facial nuclei is 105.8±3.8% in t … More he control injury group, 102.4±5.2% in the distal injury group; 94.6±7.4% in the proximal injury group, 30.9±8.3% in the brainstem injury group, 43.7±6.2% in the transplanted group. On day 28, the survival ratio is 96.4±5.0% in the control injury group, 90.2±3.0% in the distal injury group, 49.7±6.3% in the proximal injury group, 2.3±1.2% in the brainstem injury group, 20.4±5.1% in the transplanted group. In gelatin zymography and immunobistochemistry MIMiP-9 was expressed in injury site on day 1 with a peak on day 3.MMP-2 was expressed on day 1 and lasted for 7 days. In situ zymography showed gelatinase activity at the lesion site.These results suggest that the brainstem lesion model used in this study causes a massive neuronal cell death and this was partialiy rescued by the PNS autograft transplantation. MIMPs may play a role to regulate migration of inflammatory cells at the early stage of the injury.The transplantation of the PNS autograft has significant neuroprotective effects for retrograde degeneration. Less

本研究的目的是建立一种研究面神经损伤后神经元逆行性死亡机制的动物模型，并研究自体移植的周围神经组织的神经保护作用及MMPs表达的变化，包括脑干损伤模型、面神经束在脑干内的切断模型、对照损伤模型、自体移植的周围神经组织的神经保护作用及MMPs表达的变化。面神经损伤模型包括面神经损伤模型、将自体三七总皂苷移植于脑干损伤模型的损伤部位，第7天，与对侧相比，面神经核运动神经元的存活率为105.8±3.8%。 ...更多信息 对照损伤组为102.4±5.2%，近端损伤组为94.6±7.4%，脑干损伤组为30.9±8.3%，移植组为43.7±6.2%。第28天，对照组存活率为96.4±5.0%，远端损伤组为90.2±3.0%，近端损伤组为49.7±6.3%，脑干损伤组为2.3±1.2%，移植组为20.4±5.1%。明胶酶谱和免疫组化结果显示，损伤后第1天，MIMiP-9在损伤部位有表达，第3天达高峰; MMP-2在损伤后第1天开始表达，并持续7天。原位酶谱显示损伤部位有明胶酶活性，提示本研究所用的脑干损伤模型引起大量神经元细胞死亡，而自体PNS移植可部分挽救这种死亡。MIMPs可能在损伤早期参与调节炎性细胞的迁移，自体PNS移植对退行性变有明显的神经保护作用。少