The role of cell adhesion molecules in reconstruction of neural network in spinal cord and spinal nerves with special attention paid to E-cadherin and its associated protein catenin

细胞粘附分子在脊髓和脊神经神经网络重建中的作用，特别关注E-钙粘蛋白及其相关蛋白连环蛋白

• 批准号: 07671505
• 负责人: HASEGAWA Mitsuhiro
• 金额: $ 1.34万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671505/
• 关键词: E-cadherin; alpha N-catenin; Schwann cell contact; cell adhesion molecule; regeneration; remyelination; spinal cord dorsal horn; axonal sprouting; 接着分子; シュワン細胞; 神経損傷; 神経再生; サドヘリン; 脊髄後角; 座骨神経; 後根神経節

An increasing number of cell adhesive proteins is being identified as involved in cell-cell and cell-extracellular matrix interactions. Extensive studies have focused on several members of molecules including L1, N-CAM,MAG during the development and regeneration of the nervous system. We have recently found that Ca2+-dependent cell adhesion molecule E-cadherin plays an important role in the selective fasciculation of a particular subset of unmyelinated sensory fibers and that E-cadherin is exclusively expressed in lamina II of Rexed in the spinal cord dorsal horn. In this study, the temporal profile of the cellular and subcellular expression of E-cadherin and cadherin-associated protein alpha N-catenin in both spinal nerves and lamina II of the spinal cord was examined after crushing, transecting or ligaturing the sciatic nerve in mice. Peripheral nerve injury results in histological and histochemical changes in neurons and glia. Schwann cells proliferated and migrated to form Schwann … More cell columns (Bungner's bands) as initial responses to denervation, and expressed E-cadherin at their site of contact with each other and later with sprouting axons. At the initial stage of myelin formation, slender processes of a single Schwann cell interdigitated with and enveloped axons, and expressed E-cadherin at the contact site elaborated by a single Schwann cell. The expression of E-cadherin in lamina II disappeared by day 7 after axotomy and reappeared following nerve ligature (partial axonal regeneration model) on day 63. In contrast, it remained at the reduced level after nerve clipping (parmanent axonal degeneration model). The alteration of alpha N-catenin immunoreactivity showed the similar pattern. On the basis of present data, it is suggested that E-cadherin might be involved in the stabilization of peripheral glial network which provides the guidance of sprouting axons and myelination, and that E-cadherin-alpha N-catenin complex might be crucial for plasticity of the spinal cord dorsal horn after peripheral axotomy. Less

越来越多的细胞黏附蛋白被发现参与细胞-细胞和细胞-细胞外基质的相互作用。广泛的研究集中在神经系统发育和再生过程中的一些分子成员，包括L1，N-CAM，MAG。我们最近发现，钙依赖的细胞黏附分子E-钙粘附素在特定的无髓感觉纤维亚群的选择性束丛化中起重要作用，并且E-钙粘附素仅在脊髓背角的Rexed层II表达。本研究观察了挤压、切断或结扎小鼠坐骨神经后，E-钙粘蛋白及其相关蛋白α-N-catenin在脊髓II层和脊神经中的细胞和亚细胞表达的时间分布。周围神经损伤导致神经元和神经胶质细胞的组织学和组织化学改变。雪旺细胞增殖和迁移形成雪旺…更多的细胞柱(Bungner‘s带)作为对失神经支配的初始反应，在彼此接触的部位表达E-钙粘素，随后与萌发的轴突一起表达。在髓鞘形成初期，单个雪旺细胞的细长突起与轴突交错排列，包裹轴突，并在单个雪旺细胞所形成的接触部位表达E-钙粘蛋白。E-钙粘蛋白在神经切断后第7天消失，在第63天神经结扎(部分轴突再生模型)后重新出现。相反，神经夹闭(永久性轴索变性模型)后，它仍处于降低的水平。α-N-连环蛋白免疫反应的改变也表现出类似的模式。根据现有资料，提示E-钙粘蛋白可能参与了周围神经胶质网络的稳定，为轴突萌发和髓鞘形成提供了指导，而E-钙粘素-αN-连环蛋白复合体可能是脊髓背角周围神经切断后可塑性的关键因素。较少

项目成果

M. Hasegawa,: "Localization of E-cadherin in peripheral glia after nerve injury and repair" J Neuropathol Exp Neurol. 55 (4). 424-434 (1996)

M. Hasekawa，：“神经损伤和修复后外周神经胶质细胞中 E-钙粘蛋白的定位”J Neuropathol Exp Neurol。

M.Hasegawa: "Localization of E-cadherin in peripheral glia after nerve injury and repair" J Neuropathol Exp Neurol. 55(4). 424-434 (1996)

M.Hasekawa：“神经损伤和修复后外周胶质细胞中 E-钙粘蛋白的定位”J Neuropathol Exp Neurol。

HASEGAWA M: "Localization of E-cadherin in peripheral glia after herve injury and repair" J Noeiropathol Exp Neurol. 55(in press). (1996)

HASEGAWA M：“神经损伤和修复后外周胶质细胞中 E-钙粘蛋白的定位”J Noeiropathol Exp Neurol。

M.Hasegawa, A.Seto, N.Uchiyama, S.Kida, T.Yamashima, J.Yamashita: "Localization of E-cadherin in peripheral glia after nerve injury and repair" J Neuropathol Exp Neurol. 55(4). 424-434 (1996)

M.Hasekawa、A.Seto、N.Uchiyama、S.Kida、T.Yamashima、J.Yamashita：“神经损伤和修复后外周胶质细胞中 E-钙粘蛋白的定位”J Neuropathol Exp Neurol。

Seto A: "Spatiotemporal appearance of E-cadherin and αN-catehin in ceutral projection of Primary seusory nanens inspinal cond tollowing periphercal arotary." Advances in Neurotrauma Res. 7(in press). (1996)

Seto A：“E-钙粘蛋白和 αN-儿茶素在原发性脊髓神经病的中位投影中的时空表现，神经创伤研究进展 7（1996 年）。