The role of cell adhesion molecules and neurotrophic factros in reconstruction of nociceptive pathways in spinal cord and spinal nerve

细胞粘附分子和神经营养因子在脊髓和脊神经伤害感受通路重建中的作用

• 批准号: 09671413
• 负责人: HASEGAWA Mitsuhiro
• 金额: $ 1.92万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671413/
• 关键词: alpha E-catenin; alpha N-catenin; E-cadherin; immunoprecipitation; lamina II; nerve injury; NGF; Schwann cell; Rexed II; αE catenin; αN catenin; β catenin; nociception; substance-P; sciatic nerve; catenin; narve repair

Calcium dependent cell adhesion molecule, E-cadherin, plays important roles in fasciculation of a particular subset of sensory fibers, axon-Schwann cell contact and myelination after axotomy. The present study was performed to examine the expressions and localizations of E-cadherin, alpha and BETA -catenins, and actin in mouse neural tissues by immunoblot and immunohistochemical analyses, to elucidate roles of cadherin-catenin complex in establishment and maintenance of nervous system. In immunoblot analysis, E-cadherin was expressed in trigeminal nerves, spinal cords, dorsal roots, DRG, and sciatic nerves. alpha N-catenin was expressed in cerebral cortex, cerebellum, brain stem, spinal cord, and DRG.alpha E-catenin, beta -catenin and actin were expressed in all tissues that were examined. Immunoprecipitation using anti-E-cadherin antibody resulted that alpha E-catenin formed complex with E-cadherin in trigeminal nerve, DRG, and sciatic nerve, but not in spinal cord. E-cadherin- alpha … More N-catenin complex was not detected. In immunohistochemistry, E-cadherin was expressed in the dorsal and ventral root and the lamina II of the spinal cord, satellite cells and small sized neuronal cell bodies of DRG, subpopulation of unmyelinated fibers, and Schwann cells. alpha E-catenin was expressed in some of neurons, ependymal cells of central canal in spinal cord, satellite cells, some neuronal cell bodies, and Schwann cells. a N-catenin was diffusely expressed in gray matter in the spinal cord with enhanced immunoreactivity in lamina II and central canal. Satellite cells and some neuronal cell bodies were also positive. Furthermore, administration of NGF rescued the immunoreactivity of substance-P in lamina II, which is known to disappear after peripheral axotomy, but not influence that of both E-cadherin or alpha N-catenin. These results suggested that cadherin-catenin complexes regulate cell contact in various manner. Furthermore, alpha E-catenin form complex with E-cadherin in DRG, trigeminal nerves, and sciatic nerves, which plays a role in glial contact in peripheral nerves, including Schwann cells and satellite cells. Less

钙依赖性细胞粘附分子E-cadherin在轴突损伤后感觉纤维的成束、轴突-雪旺细胞接触和髓鞘形成中起重要作用。本研究采用免疫印迹和免疫组织化学方法检测了E-cadherin、α-catenins和β-catenins以及actin在小鼠神经组织中的表达和定位，以阐明cadherin-catenin复合物在神经系统建立和维持中的作用。在免疫印迹分析中，E-cadherin在三叉神经、脊髓、背根、DRG和坐骨神经中表达。α-N-连环蛋白在大脑皮层、小脑、脑干、脊髓和DRG中表达。α-E-连环蛋白、β-连环蛋白和肌动蛋白在所有检查的组织中表达。用抗E-cadherin抗体进行免疫沉淀，结果表明，在三叉神经、DRG和坐骨神经中，α E-catenin与E-cadherin形成复合物，但在脊髓中不形成复合物。E-钙粘蛋白-α ...更多信息 未检测到N-连环蛋白复合物。免疫组织化学显示，E-钙粘蛋白在脊髓背根、腹根和II层、背根节卫星细胞和小型神经元细胞体、无髓纤维亚群和施万细胞中表达。α-E-catenin表达于部分神经元、脊髓中央管室管膜细胞、卫星细胞、部分神经元胞体和雪旺细胞。a N-连环蛋白在脊髓灰质中弥漫性表达，在板层II和中央管中免疫反应性增强。卫星细胞和部分神经元胞体也呈阳性。此外，神经生长因子的管理挽救了P物质的免疫反应性在板II，这是已知的外周神经切断术后消失，但不影响这两个E-钙粘蛋白或α N-连环蛋白。这些结果表明，钙粘蛋白-连环蛋白复合物调节细胞接触的各种方式。此外，α E-连环蛋白与DRG、三叉神经和坐骨神经中的E-钙粘蛋白形成复合物，其在周围神经（包括雪旺细胞和卫星细胞）中的胶质接触中起作用。少

项目成果

Hasegawa M,Muramatsu N,Seto A,Uchiyama N,Yamashita J.: "Roles of Ca2+ dependent cell adhesion molecule E-cadherin in the nervous system" Advances in Neurotrauma Reserach. 8(1). 57-60 (1997)

Hasekawa M、Muramatsu N、Seto A、Uchiyama N、Yamashita J.：“Ca2 依赖性细胞粘附分子 E-钙粘蛋白在神经系统中的作用”神经创伤研究进展。

Hasegawa M,Muramatsu N,et al.: "Roles of Ca2+ dependent cell adhesion molecule E-cadherin in the nervous system" Advances in Neurotrauma Research. 8(1). 57-60 (1997)

长谷川 M、村松 N 等人：“Ca2 依赖性细胞粘附分子 E-钙粘蛋白在神经系统中的作用”神经创伤研究进展。

Seto A,Hasegawa M、Uchiyama N,Yamashima T,Yamashita J.: "Alteration of E-cadherin and alpha N-catenin immunoreactivity in the mouse spinal cord following peripheral axotomy" J Neuropathol Exp Neurol. 56(11). 1182-1190 (1997)

Seto A、Hasekawa M、Uchiyama N、Yamashima T、Yamashita J.：“外周轴突切除术后小鼠脊髓中 E-钙粘蛋白和 α N-连环蛋白免疫反应性的改变”J Neuropathol Exp Neurol 56(11)。 (1997)

Seto A,Hasegawa M,et al.: "Alteration of E-cadherin and alpha N-catenin immunoreactivity in the mouse spinal cord following peripheral axotomy" J Neuropathol Exp Neurol. 1182-90 (1997)

Seto A、Hasekawa M 等人：“外周轴突切除术后小鼠脊髓中 E-钙粘蛋白和 α N-连环蛋白免疫反应性的改变”J Neuropathol Exp Neurol。